Human recombinant TGF-β1 and AG1478 were from Calbiochem (La Jolla, CA, USA). EGF was kindly gifted by Serono Lab (Madrid, Spain). Human recombinant HB-EGF, human recombinant TGF-α, MβC, filipin III from Streptomyces filipinensis, nystatin and water-soluble cholesterol were from Sigma-Aldrich (St. Louis, MO, USA). The antibodies used were: mouse anti-β-actin (clone AC-15) from Sigma-Aldrich, rabbit anti-phospho-Akt (Ser473) (D9E) XP, rabbit anti-Akt, rabbit anti-phospho-EGFR (Tyr1068) (D7A5) XP, rabbit anti-EGFR, rabbit anti-phospho-p44/42 MAPK (Thr202/Tyr204), rabbit anti-p44/42 MAPK were from Cell Signaling Technology (Beverly, MA, USA), mouse anti-Caveolin-1 from BD Biosciences (Franklin Lakes, NJ, USA), rabbit anti-Ki67 from AbCam (Cambridge, UK), rabbit anti-TACE/ADAM17 (807-823) from Calbiochem, rabbit anti-NFκB p65, rabbit anti-TβRI (H-100, used in immunocytochemistry), rabbit anti-TβRI (R-20, used in western blot) and rabbit anti-TβRII (C-16) from Santa Cruz Biotechnologies (Dallas, TX, USA). Secondary antibodies: Alexa Fluor 488-conjugated anti-rabbit and anti-mouse from Molecular Probes (Eugene, OR, USA) and ECL Mouse IgG, and Rabbit IgG, HRP-Linked antibodies from GE Healthcare (Buckinghamshire, UK). GM1 was detected with horseradish peroxidase-tagged cholera toxin B subunit from Sigma (St. Louis, MO, USA).
Mouse anti caveolin 1
Manufactured by BD
Sourced in United States
Mouse anti-Caveolin-1 is a primary antibody that binds to the Caveolin-1 protein, which is a structural component of caveolae in the cell membrane. The antibody can be used in various laboratory techniques, such as Western blotting, immunohistochemistry, and immunofluorescence, to detect and quantify the Caveolin-1 protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti caveolin 1, by BD (2 mentions)
Rabbit anti tfr2, by Abcam (2 mentions)
Nystatin, by Merck Group (1 mentions)
Mouse anti β actin clone ac 15, by Merck Group (1 mentions)
Rabbit anti phospho egfr tyr1068 d7a5 xp, by Cell Signaling Technology (1 mentions)
Rabbit anti egfr, by Cell Signaling Technology (1 mentions)
Rabbit anti akt, by Cell Signaling Technology (1 mentions)
Rabbit anti phospho p44 42 mapk thr202 tyr204, by Cell Signaling Technology (1 mentions)
Rabbit anti p44 42 mapk, by Cell Signaling Technology (1 mentions)
Water soluble cholesterol, by Merck Group (1 mentions)
Filipin 3 from streptomyces filipinensis, by Merck Group (1 mentions)
Rabbit anti ki67, by Abcam (1 mentions)
Anti flotillin 1, by BD (1 mentions)
Anti transferrin receptor, by BD (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Mouse anti β tubulin, by Cell Signaling Technology (1 mentions)
Mouse anti caspase 8, by Cell Signaling Technology (1 mentions)
Rabbit anti gapdh, by Merck Group (1 mentions)
Polyvinylidene fluoride membrane, by GE Healthcare (1 mentions)
Rabbit anti lrp6 c5c7, by Cell Signaling Technology (1 mentions)
6 protocols using mouse anti caveolin 1
1
Molecular Mechanisms of TGF-β and EGFR Signaling
2
Immunochemistry analysis of caveolins
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Mouse anti-caveolin-1 (#610406), anti-transferrin receptor (# 612124), anti-flotillin-1 (#610820), anti-Fas-ligand (#556372 clone NOK-1), anti-Fas-ligand (#556387 clone G247-4), anti-fyn (#610163), and rabbit anti-caveolin-1 (#610059) antibodies were purchased from BD Biosciences (San Diego, CA, USA). Cholera toxin, peroxidase conjugate (#227041), and mouse anti-GST antibodies (#OB03 clone 8-326) were from Calbiochem. Mouse anti-Fas antibodies (#610198) were from BD Transduction Laboratories, USA. Rabbit anti-beta-tubulin antibodies (#2146) were from Cell Signaling Technology, mouse anti-caspase 8 antibodies (#551242) were from BD Pharmingen. Purified goat anti-mouse (#170-6516) and anti-rabbit (#170-6515) IgG (H+L) horseradish peroxidase conjugates were obtained from Bio-Rad (Hercules, CA, USA). Alexa Fluor® 610-R-phycoerythrin goat anti-mouse (#A20980) and fluorescein goat anti-rabbit (#F2765) IgG (H+L) were from Invitrogen (Oregon, USA).
The following cell cultures were used: human cervical adenocarcinoma HeLa cells and african green monkey kidney cells COS-1. Сell lines were from Specialist collection of continuous cell lines of vertebrates (Institute of Cytology, Russian Academy of Sciences, St-Petersburg, Russia). Cells were maintained at 37 °C and 5% CO2 in DMEM (Gibco) with 10% FCS (Hyclone).
The following cell cultures were used: human cervical adenocarcinoma HeLa cells and african green monkey kidney cells COS-1. Сell lines were from Specialist collection of continuous cell lines of vertebrates (Institute of Cytology, Russian Academy of Sciences, St-Petersburg, Russia). Cells were maintained at 37 °C and 5% CO2 in DMEM (Gibco) with 10% FCS (Hyclone).
3
Antibodies for Western Blot Analysis
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The following antibodies were used: mouse anti-β-actin (1:2000, cod. A5441, Sigma Aldrich), rabbit anti-GAPDH (1:1000, cod. G9545, Sigma Aldrich). mouse anti-caspase 8 (1:500, cod. 9746, Cell Signaling), mouse anti-β-tubulin (1:1000, cod. T5201, Sigma Aldrich), mouse anti-glutathione (1:1000, cod. MA17620, Life Technologies), mouse anti-caveolin-1 (1:1000, cod. 610406, BD).
4
Western Blot Analysis of Cellular Targets
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Protein samples dissolved in sample loading dye were separated by SDS on 8% acrylamide‐bisacrylamide gel and transferred to polyvinylidene fluoride membrane (GE Healthcare Life science, Little Chalfont, UK). Blots were blocked in 5% milk powder for 1 h at room temperature (RT) and incubated with the following antibodies: rabbit anti‐GFP ((D5.1) XP®, 1 : 1000; Cell Signaling Technology) and rabbit anti‐Lrp6 (C5C7, 1 : 1000; Cell Signaling Technology, Danvers, MA, USA), ECL Rabbit IgG (HRP‐linked F(ab)2 fragment from donkey, 1 : 2500; GE Healthcare Bio‐Sciences), rabbit anti‐TfR2 (1 : 2000; Abcam) and mouse anti‐Caveolin1 (1 : 2000; BD Transduction Laboratories, Franklin Lakes, NJ, USA), mouse anti‐β‐ catenin (1 : 2500; BD Biosciences, Franklin Lakes, NJ, USA). Cellular localization kit (Cell Signaling) was used for Histon 3B and β‐tubulin (1 : 1000). Blots are quantified with imagej (ImageJ, U. S. National Institutes of Health, Bethesda, MD, USA) plugin (Analyze→Gels).
5
Quantification of Caveolin-1 and eNOS Signaling
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Primary antibodies were mouse anti- caveolin-1 (#610406), rabbit anti-caveolin-1 (#610060) and mouse anti-NOSIII (#610296), all from BD Transduction Laboratories. Rabbit anti-phospho-eNOSSer1179 (#07–428) was from Upstate. Secondary antibodies used for immuno-blotting were anti-mouse horseradish peroxidase (anti-ms-HRP) and anti-rabbit horseradish peroxidase (anti-rb-HRP) (all from Jackson ImmunoResearch). Equal protein amounts (3–5 μg) per sample were loaded and separated using the NuPAGE (Invitrogen) systems. Separated proteins were transferred to nitrocellulose membranes (Amersham) using the NuPAGE transfer system or the iBlot Gel transfer system (Invitrogen). Proteins were detected with anti-ms-HRP or anti-rb-HRP secondary antibodies. Immunoblotted proteins were developed with ECL chemiluminescent western blotting system (Amersham) and imaged on a LAS-4000 mini system (Fujifilm). Protein chemiluminescent signals were quantified using the NIH image software (ImageJ) and expressed as arbitrary units (AU).
6
Western Blot Analysis of Protein Expression
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Samples were dissolved in 5X loading dye and separated by SDS gel electrophoresis by running on 10% acrylamide-bisacrylamide gel. Proteins were transferred to polyvinylidene fluoride (PVDF) membrane (GE Healthcare Life science, Chicago, IL, USA). Membrane blots was blocked in 5% milk powder for 45 min at room temperature (RT). Following antibodies were used at the indicated dilutions for membrane incubation. Primary antibodies: rabbit anti-GFP [(D5.1) XP, 1: 1000; Cell Signaling Technology, Danvers, MA, USA] rabbit anti-TfR2 (1:2000; Abcam, Cambridge, UK), mouse anti-Caveolin1 (1:2000; BD Transduction Laboratories, Franklin Lakes, NJ, USA) and anti-DDDDK tag antibody (ab1162, Abcam). Secondary antibodies: rabbit IgG HRP-linked F(ab′)2 fragment from donkey (1: 2500; GE Healthcare Bio-Sciences) and mouse IgG HRP Linked F(ab′)2 fragment from sheep (1:2500; GE Healthcare Bio-Sciences).
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