Plasma glucose was measured using a OneTouch® Ultra® blood glucose meter (LifeScan, Inc., Johnson & Johnson Co, Milpitas, CA). Whole blood HbA1c was measured using the Olympus AU680 clinical analyzer (Olympus America, Irving, TX). Plasma concentrations of peptides were measured using a validated two-site “sandwich” ELISA as described previously [37] (link) using two monoclonal antibodies that target the N- and C- terminal regions of exenatide. The linear range of the standard curve was 40–5,120 pg/ml, with the lower limit of quantitation at 60 pg/ml and the upper limit of quantitation at 5,000 pg/ml.
Onetouch ultra blood glucose meter
Manufactured by LifeScan
Sourced in United States
The OneTouch Ultra blood glucose meter is a handheld device used to measure the level of glucose in a person's blood. It provides a simple and accurate way for individuals to monitor their blood sugar levels.
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Lab products found in correlation
Human insulin, by Eli Lilly (2 mentions)
Glucose solution, by Merck Group (2 mentions)
Hdl cholesterol precipitating reagent, by Horiba (1 mentions)
Mouse leptin elisa kit, by Crystal Chem (1 mentions)
Ultrasensitive rat insulin elisa, by Crystal Chem (1 mentions)
Luminex 200 analyzer, by Thermo Fisher Scientific (1 mentions)
Female nod ltj mice, by Jackson ImmunoResearch (1 mentions)
Mouse adiponectin elisa kit, by Merck Group (1 mentions)
Dri chem 3500, by Fujifilm (1 mentions)
Triglyceride colorimetric assay kit, by Cayman Chemical (1 mentions)
Mouse insulin elisa, by Mercodia (1 mentions)
Stock 000662, by Charles River Laboratories (1 mentions)
Mouse ultrasensitive insulin elisa, by ALPCO (1 mentions)
Mouse leptin elisa, by Crystal Chem (1 mentions)
Mouse elisa kit, by Abcam (1 mentions)
D glucose, by Merck Group (1 mentions)
Mouse insulin elisa kit, by Mercodia (1 mentions)
Humulin, by Eli Lilly (1 mentions)
Bx51 fluorescence microscope, by Olympus (1 mentions)
29 protocols using onetouch ultra blood glucose meter
1
Plasma Glucose, HbA1c, and Peptide Measurement
2
Streptozotocin-Induced Diabetes in Rats
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Male Sprague-Dawley rats (7–8 weeks old, weighing 200 ± 20 g) were purchased from Jinan Pengyue Experimental Animal Breeding Co. Ltd (Jinan, China). They were housed in a room with a 12/12 h light/dark cycle and an ambient temperature of 23°C ± 3°C. All animal experiments were carried out according to the National Institute of Health Guideline for the Care and Use of Laboratory Animals, and performed by the Animal Ethics Committee of School of Pharmacy and Pharmaceutical Sciences & Institute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences (2017036, Jinan, China).
The rats were given a single intraperitoneal injection with a freshly prepared solution of STZ (60 mg/kg) in 0.1 mol/L citrate buffer (pH = 4.4) to induce diabetes (Seke Etet et al., 2017 (link); Zhang et al., 2017 (link)). After 8 weeks, the rats with fasting blood glucose (FBG) for 5–6 h exceeding 16.7 mmol/L were considered successful diabetes models. The FBG was measured from the tail vein using a One-Touch Ultra® Blood Glucose Meter (LifeScan Inc., Milpitas, United States).
The rats were given a single intraperitoneal injection with a freshly prepared solution of STZ (60 mg/kg) in 0.1 mol/L citrate buffer (pH = 4.4) to induce diabetes (Seke Etet et al., 2017 (link); Zhang et al., 2017 (link)). After 8 weeks, the rats with fasting blood glucose (FBG) for 5–6 h exceeding 16.7 mmol/L were considered successful diabetes models. The FBG was measured from the tail vein using a One-Touch Ultra® Blood Glucose Meter (LifeScan Inc., Milpitas, United States).
3
Insulin and Glucose Tolerance Tests
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The insulin tolerance tests (ITT) were carried out with WT (n = 6) and Adipoq−/− (n = 7) male mice. Blood glucose concentration was measured using OneTouch Ultra blood glucose meter and test strips (LifeScan). For ITT, mice were i.p. injected with human insulin (Eli Lilly) at a dose of 1.0 U/kg of body weight following a 6 h of fasting period in the morning. Blood glucose was assessed at 0, 30, 60, 90 and 120 min after injections. For glucose tolerance tests (GTT), mice were i.p. injected with glucose at a dose of 2.0 g/kg body weight following a 18 h overnight fasting. The blood glucose was measured at 0, 15, 30, 60 and 120 min after injection.
4
Comprehensive Eye Examination Protocol
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All participants were invited to a temporary clinic for examination. Relevant personal and demographic details and examination findings were recorded.
The examination flow chart (Fig.1 ; adapted [17 (link)]) indicates the data collected by the team members. All participants had presenting and best-corrected visual acuity (VA) measured with a reduced logMAR tumbling E-chart, automated refraction and keratometry (Takagi ARKM-100, Takagi Seiko, Japan), frequency doubling technology (FDT) visual function testing (Carl Zeiss Meditec AG Jena Germany) and ultrasound A-scan biometry (Bioline Biometer OPTIKON 2000 S.p.A Roma, Italy). All participants had basic eye examination performed by the first ophthalmologist, and detailed ocular examination was performed by the second ophthalmologist: in those with VA of worse than 20/40 in one or both eyes; vertical cup:disc ratio (VCDR) ≥0.6 in one or both eyes or VCDR asymmetry of ≥0.2, or any retinal abnormality seen on undilated fundoscopy [17 (link)]. In addition, a subsample of 1-in-7 participants who also had the detailed examination regardless of their VA had a random blood glucose (RBG) test (OneTouch Ultra blood glucose meter, LifeScan UK).![]()
The examination flow chart (Fig.
The Nigeria Blindness Survey examination flow chart
5
Comprehensive Metabolic Profiling in Rodents
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Blood glucose was determined at baseline and weekly thereafter using the OneTouch Ultra blood glucose meter (LifeScan). Plasma insulin levels were analyzed using an ELISA for rat insulin (Ultra Sensitive Rat Insulin ELISA; Crystal Chem). Plasma leptin levels were determined using a mouse leptin ELISA kit (Crystal Chem). Quantitative determination of mouse MCP-1 levels in plasma was performed by using an ELISA kit (R&D Systems). Plasma cholesterol was determined using a cholesterol reagent colorimetric assay kit (Roche Diagnostics). For determination of plasma HDL cholesterol levels, plasma was incubated with an HDL cholesterol precipitating reagent (Pointe Scientific, Canton, MI) followed by separation of HDL by centrifugation (2,000g, 10 min). HDL was then quantified using an enzymatic cholesterol detection kit (Roche Diagnostics).
6
Gluconeogenesis Assay in Mice
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Gluconeogenesis tests were performed as described previously (43 (link), 44 ). Briefly, mice were fasted overnight (16 h) prior to injected i.p. with pyruvate (2 g/kg BW), glycerol (2 g/kg BW), lactate (1.5 g/kg BW), fructose (2 g/kg BW), or glutamine (1.5 g/kg BW), respectively. 5′-AMP (0.5 μmol/g i.p.) or adenosine (0.2 μmol/g i.p.) was mixed with gluconeogenic substrates and coinjected into mice. Blood glucose levels were determined from the tail vein at 0, 15, 30, 60, and 120 min after injection, with a One Touch Ultra Blood Glucose Meter (Lifescan).
7
Oral Glucose Tolerance Test in Mice
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At week 5, oral glucose tolerance test (GTT) was conducted with that the blood glucose level of overnight-fasted mice was measured at 0, 30, 60, and 120 min after glucose (1 g kg−1 BW) oral administration. The tail blood glucose was detected by an OneTouch ultra blood glucose meter (LifeScan, Milpitas, USA).
8
Measuring Glucose and Insulin Levels
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During the process of establishing the model, blood glucose was measured once per week. After fasting for 12 h, blood was collected from the tail vein and the concentration of blood glucose in individual mice was measured using a OneTouch Ultra blood glucose meter (LifeScan, Milpitas, Santa Clara, CA, USA). For the measurement of serum insulin, blood was collected from the internal iliac vein at the end of the experiment, and the plasma was centrifuged (3500 rpm for 10 min) at 4 °C and the supernatant was collected. Then, the insulin level in serum was measured by radioimmunoassay.
9
Diabetic Murine Wound Healing Model
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The animal studies were conducted in accordance with the procedure guidelines approved by the University of British Columbia Animal Care Committee. All mice used in the studies were purchased from the Jackson Laboratories (stock number 000642; Bar Harbor, ME, USA) at 7 weeks. All mice used were from a C57BLKS/J background strain, male and either homozygous or heterozygous for the spontaneous mutation in the leptin receptor. The mice homozygous for the leptin receptor mutation were considered to be diabetic. The mice heterozygous for the leptin receptor mutation were considered to be the non-diabetic littermates. All mice were fed standard laboratory chow (equal parts of PicoLab Mouse Diet 20 #5058 and PicoLab Rodent Diet 20 #5053 from LabDiet, Richmond, IN, USA) and water ad libitum.
Two days before the wound-healing surgery, all mice were fasted for 4 h and fasting blood glucose levels of the mice were determined with OneTouch Ultra Blood Glucose Meter (LifeScan, New Brunswick, NJ, USA). Only mice with fasting blood glucose levels >200 mg/dl were considered diabetic. The hair on the backs of the animals were removed by shaving with hair clippers followed by treatment with Nair (Church and Dwight, Ewing, NJ, USA) for 90 s. Finally, only mice with no visible active hair follicles were subjected to surgery.
Two days before the wound-healing surgery, all mice were fasted for 4 h and fasting blood glucose levels of the mice were determined with OneTouch Ultra Blood Glucose Meter (LifeScan, New Brunswick, NJ, USA). Only mice with fasting blood glucose levels >200 mg/dl were considered diabetic. The hair on the backs of the animals were removed by shaving with hair clippers followed by treatment with Nair (Church and Dwight, Ewing, NJ, USA) for 90 s. Finally, only mice with no visible active hair follicles were subjected to surgery.
10
Multiparametric Metabolic Profiling
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