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Mouse monoclonal antibody against human cd63 h5c6

Mouse monoclonal antibody against human CD63 (H5C6) is a laboratory reagent used for the identification and detection of the CD63 protein, a member of the tetraspanin family, expressed on the surface of various cell types. This antibody can be used in techniques such as flow cytometry, immunohistochemistry, and immunoprecipitation to study the expression and distribution of CD63 in biological samples.

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2 protocols using mouse monoclonal antibody against human cd63 h5c6

1

Antibody Reagents for Extracellular Vesicle Analysis

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Antibodies to β-actin and HA tag were purchased from Cell Signaling Technologies. Antibodies against double stranded DNA and Histone-H3 (tri-methyl K9) were purchased from Abcam. β1-integrin and α-tubulin antibodies were purchased from MilliporeSigma. CD63 antibody used for immunoblotting was obtained from System Biosciences International. VAMP3, ARF6, Emerin, APEX1, Annexin-A1, Flotillin, Alix, CD81, TSG-101, SRY, and Histone H-3 antibodies, along with rabbit polyclonal antibody against cGAS, were purchased from Proteintech. Mouse monoclonal antibody against human CD63 (H5C6) used for immunofluorescence was purchased from the Developmental Studies Hybridoma Bank. Antibody against GFP was purchased from Life Technologies. Fluorophore conjugated secondary antibodies (donkey anti-mouse Alexa Fluor Plus 488, donkey anti-rabbit Alexa Fluor Plus 555, donkey anti-mouse Alexa Fluor Plus 680, and donkey anti-rabbit Alexa Fluor Plus 800), rhodamine phalloidin, Alexa Fluor 647-phalloidin, To-Pro-3 iodide, Nuc Green stain, and DRAQ-5 were purchased from Life Technologies. High capacity magnetic sepharose beads were purchased from MilliporeSigma. Unless noted, all other chemicals were purchased from V.W.R.
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2

Antibody Reagents for Extracellular Vesicle Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies to β-actin and HA tag were purchased from Cell Signaling Technologies. Antibodies against double stranded DNA and Histone-H3 (tri-methyl K9) were purchased from Abcam. β1-integrin and α-tubulin antibodies were purchased from MilliporeSigma. CD63 antibody used for immunoblotting was obtained from System Biosciences International. VAMP3, ARF6, Emerin, APEX1, Annexin-A1, Flotillin, Alix, CD81, TSG-101, SRY, and Histone H-3 antibodies, along with rabbit polyclonal antibody against cGAS, were purchased from Proteintech. Mouse monoclonal antibody against human CD63 (H5C6) used for immunofluorescence was purchased from the Developmental Studies Hybridoma Bank. Antibody against GFP was purchased from Life Technologies. Fluorophore conjugated secondary antibodies (donkey anti-mouse Alexa Fluor Plus 488, donkey anti-rabbit Alexa Fluor Plus 555, donkey anti-mouse Alexa Fluor Plus 680, and donkey anti-rabbit Alexa Fluor Plus 800), rhodamine phalloidin, Alexa Fluor 647-phalloidin, To-Pro-3 iodide, Nuc Green stain, and DRAQ-5 were purchased from Life Technologies. High capacity magnetic sepharose beads were purchased from MilliporeSigma. Unless noted, all other chemicals were purchased from V.W.R.
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