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Antimouse pd l1 clone 10f 9g2

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Antimouse PD-L1 (clone 10F.9G2) is a monoclonal antibody that binds to the mouse programmed cell death-ligand 1 (PD-L1) protein. It is commonly used in research applications to modulate the PD-1/PD-L1 immune checkpoint pathway.

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Lab products found in correlation

Isotype rat igg2b clone ltf 2, by BioXCell (1 mentions) Pd 10 column, by GE Healthcare (1 mentions) P scn bn nota, by Macrocyclics (1 mentions) P scn bn dota, by Macrocyclics (1 mentions) Be0101, by BioXCell (1 mentions) Mk 2206, by Selleck Chemicals (1 mentions) Renca, by American Type Culture Collection (1 mentions) Atezolizumab, by InvivoGen (1 mentions) Human pd1 fc, by R&D Systems (1 mentions) Hrp conjugated anti human igg, by Promega (1 mentions) Ifn γ, by Thermo Fisher Scientific (1 mentions) Phorbol 12 myristate 13 acetate pma, by Merck Group (1 mentions) Igg2b isotype control antibodies, by BioXCell (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Mitomycin c, by Merck Group (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) Mcf 7, by American Type Culture Collection (1 mentions) Mouse cd80 fc, by R&D Systems (1 mentions)

6 protocols using antimouse pd l1 clone 10f 9g2

1

Radiolabeling of PD-1 and PD-L1 Antibodies

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Antimouse PD-L1 (clone 10F.9G2)
and its isotype rat IgG2b (clone LTF-2) and antimouse PD-1 (CD279,
clone J43) and its isotype polyclonal Armenian hamster IgG were obtained
from Bio X Cell (West Lebanon, NH). Bifunctional chelators 2-(4-isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane
tetraacetic acid (p-SCN-Bn-DOTA) and 2-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic
acid (p-SCN-Bn-NOTA) were obtained from Macrocyclics
(Plano, TX). PD-10 columns were purchased from GE Healthcare (Pittsburgh,
PA). [64Cu]CuCl2 (specific activity, 21.42 Ci/μmol)
was obtained from the Cyclotron Radiochemistry Facility at The University
of Texas MD Anderson Cancer Center (Houston, TX).
Zhao J., Wen X., Li T., Shi S., Xiong C., Wang Y.A, & Li C. (2020). Concurrent Injection of Unlabeled Antibodies Allows Positron Emission Tomography Imaging of Programmed Cell Death Ligand 1 Expression in an Orthotopic Pancreatic Tumor Model. ACS Omega, 5(15), 8474-8482.
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2

Melanoma and Colorectal Tumor Immunotherapy

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Mice were injected s.c. with 5 × 105 B16-OVA melanoma cells or 5 × 105 MC38 tumor cells and administered either 0.5 mg (B16-OVA) or 0.25 mg (MC38) of anti-mouse PD-L1 (Clone10F.9G2; BE0101), anti-mouse CTLA4 (Clone 9H10, BE0131), corresponding IgG2b (LTF-2; BE0090) or polyclonal Syrian hamster IgG (BE0087) control (all from BioXCell, USA) every 3 days starting from day 3 of tumor challenge according to ref. [30 (link), 32 (link)].
Klepsch V., Pommermayr M., Humer D., Brigo N., Hermann-Kleiter N, & Baier G. (2020). Targeting the orphan nuclear receptor NR2F6 in T cells primes tumors for immune checkpoint therapy. Cell Communication and Signaling : CCS, 18, 8.
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3

Comparison of Human and Mouse Renal Cell Carcinoma

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The human RCC cell line 786‐O and mouse RCC cell line RENCA were obtained from American Type Culture Collection (Rockville, MD, USA). Both cell lines were cultured in RPMI 1640 supplemented with 10% fetal bovine serum, 100 IU/mL penicillin, and 100 μg/mL streptomycin. They were grown in a humidified incubator with 5% CO2 at 37°C. EVE and MK2206 were purchased from Selleck (San Diego, CA, USA). Rapamycin and LY294002 were purchased from Calbiochem (LaJolla, CA, USA). Anti‐mouse PD‐L1 (clone 10F.9G2) and IgG isotype control (clone LTF‐2) were purchased from Bio‐XCell (West Lebanon, NH, USA).
Hirayama Y., Gi M., Yamano S., Tachibana H., Okuno T., Tamada S., Nakatani T, & Wanibuchi H. (2016). Anti‐PD‐L1 treatment enhances antitumor effect of everolimus in a mouse model of renal cell carcinoma. Cancer Science, 107(12), 1736-1744.
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4

Antibody-based Detection and Signaling Protocols

Cited 1 time
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The following antibodies were used: anti-human PD-L1 human mAb [9]; anti-human PD-L1 humanized mAb Atezolizumab (InvivoGen, San Diego, USA); HRP-conjugated anti-human IgG (Promega, Madison, USA); HRP-conjugated anti-human IgG (Fab’)2 goat monoclonal antibody (Abcam, Cambridge, UK); HRP-conjugated anti-rabbit immunoglobulin from goat antiserum (Thermo Fisher Scientific, Meridian Road, USA). Anti-human p-Erk rabbit polyclonal antibody; anti-human p-P38 rabbit polyclonal antibody; anti-human p-JNK rabbit polyclonal antibody; anti-human Cleaved Caspase-3 rabbit polyclonal antibody (all from Cell Signaling, Danvers, USA); Erb-hcAb, human anti-ErbB2 compact Antibody21 (link). Anti-mouse PD-L1 (clone 10F.9G2, BioXcell, West Lebanon, USA). The following recombinant proteins were used: human PD-1/Fc (R&D Systems, USA); IFN γ (Peprotech, USA).
Passariello M., D’Alise A.M., Esposito A., Vetrei C., Froechlich G., Scarselli E., Nicosia A, & De Lorenzo C. (2019). Novel Human Anti-PD-L1 mAbs Inhibit Immune-Independent Tumor Cell Growth and PD-L1 Associated Intracellular Signalling. Scientific Reports, 9, 13125.
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5

Breast Cancer Cell Culture Protocol

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MDA-MB-231 (highly metastatic) and MCF7 (nonmetastatic) human breast cancer cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). Mouse breast cancer cells (4T1-WT, mock and NDRG2) were kindly provided by Professor KD Kim at Gyeongsang National University in Jinju, Korea. All cell lines were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM; Gibco/Invitrogen, Carlsbad, CA, USA) containing 10% heat-inactivated fetal bovine serum (FBS) (Gibco/Invitrogen) and 1% penicillin/streptomycin (Gibco/Invitrogen). The cells were maintained in an atmosphere of 5% CO2 in a 37 °C humidified incubator. PMA (phorbol 12-myristate 13-acetate) and mitomycin C were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-mouse PD-L1 (clone 10F.9G2) and IgG2b isotype control antibodies were purchased from BioXcell (West Lebanon, NH, USA).
Lee A., Lim S., Oh J., Lim J., Yang Y., Lee M.S, & Lim J.S. (2021). NDRG2 Expression in Breast Cancer Cells Downregulates PD-L1 Expression and Restores T Cell Proliferation in Tumor-Coculture. Cancers, 13(23), 6112.
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6

Tumor-directed immune modulation therapy

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Female 6 week old BALB/c or C57BL/6 mice were inoculated s.c. in the flank on day ~−7 with 5×105 CT26 colon carcinoma or 1×106 B16F10 melanoma cells, respectively. Intra-tumoral (i.t.) injections of mouse CD80-Fc (20μg/50μl/mouse, R&D Systems), rat IgG2a clone 2A3 (20μg/50μl/mouse, BioXCell), or CpG ODN 1555 (100μg/50μl/mouse) were started on day 0 when tumors were approximately 5mm in diameter and administered twice a week for three weeks (7 (link)). For some experiments mice were inoculated on ~day −7 with B16F10 cells on both flanks and tumors on only one flank were treated. Intraperitoneal (i.p.) injections of mouse CD80-Fc (200ug/mouse, R&D Systems), rat IgG2a clone 2A3 (200ug/mouse BioXCell), or anti-mouse PDL1 clone 10F.9G2 (200ug/mouse BioXCell) were administered on days 3, 6, 9, and 22 following tumor inoculation. Mice were sacrificed when they became moribund and their tumors were cryopreserved and analyzed by immunohistochemistry. Tumors were measured every 2–3 days in two perpendicular diameters. Tumor volume = 4/3πr3 where r = (diameter 1 + diameter 2)/4.
Horn L.A., Long T.M., Atkinson R., Clements V, & Ostrand-Rosenberg S. (2017). Soluble CD80 protein delays tumor growth and promotes tumor infiltrating lymphocytes. Cancer immunology research, 6(1), 59-68.
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