Western blot analyses were performed as previously described (X. P. Chen et al., 2016 ) with the following primary antibodies: anti‐endocan (0.1 μg/ml; R&D Systems); anti‐ERK1/2 (1:1,000; Cell Signaling Technology); anti‐p‐ERK1/2 (1:1,000; Cell Signaling Technology); anti‐VEGF (1:1,000; Abcam); anti‐VEGFR1 (1:1,000; Abcam); anti‐VEGFR2 (1:1,000; Cell Signaling Technology); and GAPDH (1:1,000; Cell Signaling Technology).
Anti endocan
Manufactured by R&D Systems
Sourced in United States
Anti‐endocan is a recombinant protein that binds to and neutralizes endocan, a proteoglycan involved in various biological processes. This product can be utilized in research applications to study the role of endocan in cellular and molecular mechanisms.
Automatically generated - may contain errors
Lab products found in correlation
Anti erk1 2, by Cell Signaling Technology (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Anti vegfr1, by Abcam (1 mentions)
Anti vegfr2, by Cell Signaling Technology (1 mentions)
Anti vegf, by Abcam (1 mentions)
Anti p erk1 2, by Cell Signaling Technology (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Anti actin, by Merck Group (1 mentions)
Vascular endothelial growth factor (vegf), by Santa Cruz Biotechnology (1 mentions)
Vcam 1, by Cell Signaling Technology (1 mentions)
E selectin, by Santa Cruz Biotechnology (1 mentions)
Sdf 1, by Cell Signaling Technology (1 mentions)
2 protocols using anti endocan
1
Western Blot Analysis of Angiogenic Markers
2
Western Blot Analysis of Angiogenic Factors
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Total cell or tissue lysates were extracted using lysis buffer, and proteins were separated in 8-12% (v/v) SDS-PAGE gels. After electrophoresis (Bio-Rad Laboratories, Hercules, CA, USA), the proteins were transferred onto PVDF membranes (Millipore, Darmstadt, Germany), and the membranes were incubated with anti-endocan (R&D Systems, AF1810, Minneapolis, MN, USA), VEGF (Santa Cruz Biotechnologies, sc-7269, Dallas, TX, USA), stromal cell-derived factor (SDF)-1 (Cell Signaling, Danvers, #3740, MA, USA), vascular cell adhesion molecule-1 (VCAM-1; Cell Signaling, #13,662, Danvers, MA, USA), intercellular adhesion molecule-1 (ICAM-1; Cell Signaling, #67,836, Danvers, MA, USA), E-selectin (Santa Cruz Biotechnologies, sc-137054, Dallas, TX, USA), anti-actin (Merck, 3,423,208, Darmstadt, Germany) at 4 ℃ overnight. After washing three times, the membranes were incubated with HRP-conjugated secondary antibodies for 1 h at room temperature. Finally, the membranes were visualized using the ECL kit.
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