Metoprolol tartrate was obtained from AstraZeneca Pharmaceutical Co., Ltd. (Jiangsu, China). Barium chloride was obtained from Maoye Chemical Reagent Co., Ltd. (Chongqing, China). LC grade acetonitrile was purchased from Merck (Merck, Darmstadt, Germany). Isopropanol, formic acid and metabolite standards were purchased from Sigma-Aldrich (Sigma-Aldrich, St Louis, MO, USA). Purified water (18.2 MΩ) was prepared from a Mill-Q purification system (Millipore Corp., MA, USA).
Mill q purification system
Manufactured by Merck Group
Sourced in United States
The Mill-Q purification system is a water purification device designed to produce high-quality, ultrapure water for laboratory applications. It utilizes a multi-stage filtration process to remove impurities and contaminants, ensuring the delivery of consistently pure water for various experimental and analytical needs.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Avantor (2 mentions)
Hplc grade formic acid, by Avantor (2 mentions)
Metabolite standards, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Isopropanol, by Merck Group (1 mentions)
Metoprolol tartrate, by AstraZeneca (1 mentions)
Lc grade acetonitrile, by Merck Group (1 mentions)
Lysozyme, by Solarbio (1 mentions)
Chloroform, by Avantor (1 mentions)
Ethanol, by Avantor (1 mentions)
Acetic acid, by Avantor (1 mentions)
Penicillin streptomycin solution, by Pfizer (1 mentions)
L glutamine, by Pfizer (1 mentions)
Hepes buffer, by Pfizer (1 mentions)
4 3 4 iodophenyl 2 4nitrophenyl 2h 5 tetrazolium 1 3 benzenedisulfonate wst 1 assay, by Roche (1 mentions)
5 and 6 chloromethyl 2 7 dichlorodihydrofluorescein diacetate, by Thermo Fisher Scientific (1 mentions)
Acetyl ester cm h2dcfda, by Thermo Fisher Scientific (1 mentions)
Acetone, by Merck Group (1 mentions)
Gelatin, by Merck Group (1 mentions)
Hexane, by Merck Group (1 mentions)
4 protocols using mill q purification system
1
Metoprolol Tartrate Quantification Protocol
2
Copper-catalyzed SPOP ELISA Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A human SPOP ELISA kit was purchased from Shanghai Jianglai Bio-Technology Co., Ltd (Shanghai, China). Copper chloride (CuCl2), L-Aspartic acid (L-Asp), sodium hydroxide (NaOH), hydrochloric acid (HCl), hydrogen peroxide (H2O2), sodium tetrachloropalladate (II) (Na2PdCl4), chloroplatinic acid (H2PtCl6·6H2O) was obtained from Aladdin (Shanghai, China). Lysozyme was obtained from Solarbio Life Science (Beijing, China). Glutathione (GSH), Horseradish Peroxidase (HRP), Catalase (CAT) were purchased from Sigma-Aldrich (St Louis, USA). IOSE80 cell line was obtained from Shengzhen Huatuo Bio-Technology Co., Ltd (Shengzhen, China). The other reagents used in this experiment were of analytical grade and all of the water used in the tests was obtained from a Millipore Mill-Q purification system(> 18.2 MΩ cm, USA).
3
Extraction and HPLC Analysis of Lawsone
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ethanol, chloroform, acetic acid, HPLC-grade formic acid, and mEthanol were purchased from VWR (Milan, Italy). HPLC-grade water (18 mΩ) was obtained by a Mill-Q purification system (Millipore Corp., Burlington, MA, USA). Standard lawsone (purity 97%) was purchased from Sigma-Aldrich (Milan, Italy).
4
Oxidative Stress Evaluation in Cell Cultures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hexane, acetone, and Folin-Ciocalteau reagent, and gelatin were purchased from Sigma-Aldrich (Milan, Italy). HPLC grade formic acid and methanol were purchased from VWR (Milan, Italy). HPLC grade water (18 mΩ) was obtained by a Mill-Q purification system (Millipore Corp., Bedford, MA, USA).
H2O2 was purchased from Farmac-Zabban S.p.a. (Calderara di Reno, BO, Italy). Medium 199 (M199), fetal bovine serum (FBS), penicillin-streptomycin solution, L-glutamine, and HEPES buffer were supplied by Hospira S.r.l. (Naples, Italy).
4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolium]-1,3-benzenedisulfonate (WST-1 assay), was purchased from Roche Applied Science (Mannheim, Germany), 5-(and-6)-chloromethyl-2′, 7′-dichloro-di-hydro-fluorescein diacetate, and acetyl ester (CM − -H2DCFDA) were supplied by Thermo Fisher Scientific Inc. (Waltham, MA, USA).
H2O2 was purchased from Farmac-Zabban S.p.a. (Calderara di Reno, BO, Italy). Medium 199 (M199), fetal bovine serum (FBS), penicillin-streptomycin solution, L-glutamine, and HEPES buffer were supplied by Hospira S.r.l. (Naples, Italy).
4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolium]-1,3-benzenedisulfonate (WST-1 assay), was purchased from Roche Applied Science (Mannheim, Germany), 5-(and-6)-chloromethyl-2′, 7′-dichloro-di-hydro-fluorescein diacetate, and acetyl ester (CM − -H2DCFDA) were supplied by Thermo Fisher Scientific Inc. (Waltham, MA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!