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Antihumantnf α

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AntihumanTNF-α is a monoclonal antibody that binds to human Tumor Necrosis Factor-alpha (TNF-α). TNF-α is a pro-inflammatory cytokine involved in various immune and inflammatory responses.

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Lab products found in correlation

Hla dr fitc, by BioLegend (1 mentions) Cd4 percp cy5, by BD (1 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Ccr7 pe cf594, by BD (1 mentions) Cd69 bv650, by BioLegend (1 mentions) Cd38 apc cy7, by BioLegend (1 mentions) Il 2 bv711, by BD (1 mentions) Cxcr5alexa647, by BD (1 mentions) Cd45ro apc h7, by BD (1 mentions) Cd20alexa700, by BD (1 mentions) Il 21 pe, by Thermo Fisher Scientific (1 mentions) Cd27 pecy5, by Thermo Fisher Scientific (1 mentions) Cd45rope texasred, by Beckman Coulter (1 mentions) Recombinant human il 21, by R&D Systems (1 mentions) Cd10 pe cy7, by BD (1 mentions) Ifn γ pe cy7, by BD (1 mentions) Cd40l bv605, by BioLegend (1 mentions) Tnf α apc cy7, by BioLegend (1 mentions) Igd fitc, by BD (1 mentions) Cd21 pe cy5, by BD (1 mentions)

3 protocols using antihumantnf α

1

Flow Cytometry of Immune Cell Subsets

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The following fluorochrome conjugated antihuman monoclonal antibodies (MoAbs) were used for flow cytometry studies: ICOSBV421, ICOSAlexa488, CD40LBV605, CD69BV650, HLA-DRFITC, CD38APCCy7, and TNF-αAPCCy7 from BioLegend (San Diego, CA); CD3BUV395, CD4PerCPCy5.5, CD8Alexa-Fluor700, CCR7PECF594, IL-2BV711, CXCR5Alexa647, IFNγPE-Cy7, PD1BV650, CD45ROAPCH7, CD21PECy5, CD27PerCPCy5.5, IgDFITC, CD10PECy7, and CD20Alexa700 from BD Bioscience (San Jose, CA); IL-21PE, CD27PECy5, and IL-17Alexa488 from e-Biosciences (San Diego, CA); and CD45ROPE-TexasRed from Beckman Coulter (Fullerton, CA). Live/Dead Fixable Aqua Dead Cell Stain Kit and CellTrace Violet Cell Proliferation Kit were from ThermoFisher (Boston, MA). Recombinant human IL-21 (Cat #8879-IL-010) from R&D systems, purified antihuman IL-2 (Cat #3440-ON-500) and antihuman-IL-21 (Cat #MT216G/21.3m) from Mabtech, and antihumanTNF-α (Cat #502922) from BioLegend were used. Samples were acquired on a BD LSRFortessa (BD Biosciences, CA) flow cytometer and analyzed by FlowJo V10 (TreeStar, Inc).
Pallikkuth S., de Armas L.R., Rinaldi S., George V.K., Pan L., Arheart K.L., Pahwa R, & Pahwa S. (2019). Dysfunctional peripheral T follicular helper cells dominate in people with impaired influenza vaccine responses: Results from the FLORAH study. PLoS Biology, 17(5), e3000257.
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2

ELISA Quantification of TNF-α

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Analysis of TNF-α protein released from hMDMs was performed with a sandwich ELISA, as previously described.82 (link) Purified and biotin-labeled antihuman TNF-α and protein standards were purchased from BioLegend (San Diego, CA).
Genito C.J., Eckshtain-Levi M., Piedra-Quintero Z.L., Krovi S.A., Kroboth A., Stiepel R.T., Guerau-de-Arellano M., Bachelder E.M, & Ainslie K.M. (2021). Dexamethasone and Fumaric Acid Ester Conjugate Synergistically Inhibits Inflammation and NF-κB in Macrophages. Bioconjugate chemistry, 32(8), 1629-1640.
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3

Profiling Urine Cell Immune Signatures

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Cryopreserved patient post-BCG urine cells were thawed and resuspended in cR-10 media at 10×106 cells/mL then passed through 100 µm strainer. Urine cell suspensions were either directly stained with flow cytometry antibody (for T cells and activation markers) or incubated with PMA/Ionomycin/Golgi Blocker (BioLegend, Cell Activation Cocktail) for 4 hours, followed by cell surface staining for T cells and then intracellular staining for cytokines. Flow staining antibodies/dye used were as follows: Fc blocker/Human TruStain FcX (BioLegend), fixable viability dye eFluor455UV (Thermo Fisher Scientific/eBioscience), anti-human CD45 (BioLegend, clone HI30), anti-human CD3, anti-human CD4, anti-human CD8, anti-human γδ TCR, anti-human TCR Vγ9 (BioLegend, clone B3), anti-human TCR Vδ2 (BioLegend, clone B6), anti-human CCR2 (BioLegend, clone KO36C2), anti-human CD44 (BioLegend, clone IM7), anti-human CD107a, anti-human CD56 (BioLegend, clone 5.1H11), anti-human IFNγ, anti-human TNFα, anti-human Granzyme B (BioLegend, clone GB11), anti-human Perforin (BioLegend, clone dG9). Fixed and stained urine cell samples were then passed through a 35 µm filter cap on a flow tube to remove any remaining debris before they were analyzed on an LSR II cytometer (BD).
Ji N., Mukherjee N., Reyes R.M., Gelfond J., Javors M., Meeks J.J., McConkey D.J., Shu Z.J., Ramamurthy C., Dennett R., Curiel T.J, & Svatek R.S. (2021). Rapamycin enhances BCG-specific γδ T cells during intravesical BCG therapy for non-muscle invasive bladder cancer: a randomized, double-blind study. Journal for Immunotherapy of Cancer, 9(3), e001941.
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