Spc a 1

Human NSCLC cell lines (SPC-A-1, 95D, A549, H460 and H1299) were purchased from the Shanghai Cell Bank of Chinese Academy of Science (Shanghai, China) and maintained in complete medium with high-glucose RPMI-DMEM (Hyclone, GE Healthcare Life Sciences, Piscataway, NJ, USA) and 10% fetal bovine serum (FBS, Gibco, Grand Island, NY, USA). An immortalised bronchial epithelial cell HBE and 293T cell were cultured in RPMI-1640 (Hyclone) with 10% FBS. All cells were incubated at 37 °C in a humidified atmosphere containing 5% CO₂.

The human bronchial epithelial (HBE) cell line was purchased from the American Type Culture Collection (Manassas, VA, USA). The A549, H1299, H460, 973, 83A, LK2, and SPC-A1 cell lines were obtained from the Shanghai Cell Bank (Shanghai, China). All cell lines were validated by short tandem repeat DNA genotyping. All cells were cultured in Roswell Park Memorial Institute 1640 (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific), 100 IU/mL penicillin (Sigma, St Louis, MO, USA), and 100 μg/mL streptomycin (Sigma). Details of cell culture were described previously.13 (link)

Three NSCLC cell lines (i.e.A549, H1299, H460 and SPC-A-1) and normal bronchial epithelial cell line (i.e. HBE) were purchased from American Type Culture Collection (Manassas, USA), and another NSCLC cell line (i.e. SPC-A-1) was taken from Shanghai Cell Bank of Chinese Academy of Sciences (China). The cells were cultured within high-glucose DMEM complete medium (Hyclone, USA) that included 10% fetal bovine serum (FBS), 100U/ml penicillin and 100 Lg/ml streptomycin. Then we maintained the cells in 5% CO₂ and saturated humidity at 37°C. The culture solution was changed daily, and cells were managed with secondary culture at the ratio of 1:2 every 3 days.