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Cd45r clone ra3 6b2

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The CD45R clone RA3-6B2 is a monoclonal antibody that specifically binds to the CD45R antigen expressed on the surface of B cells, T cells, and other leukocytes. It is a widely used tool in flow cytometry and other immunological applications for the identification and characterization of these cell populations.

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Lab products found in correlation

Cd8 clone 53 6, by BioLegend (2 mentions) Streptavidin apc, by Thermo Fisher Scientific (1 mentions) Igg2a isotype control, by BioXCell (1 mentions) Flt3l, by R&D Systems (1 mentions) Cd103 clone 2e7, by BioLegend (1 mentions) Cd3 clone 17a2, by BioLegend (1 mentions) Cd69 clone h1.2f3, by Thermo Fisher Scientific (1 mentions) Nk1.1 clone pk136, by BioXCell (1 mentions) Cd4 clone rm4 5, by BioLegend (1 mentions) Cd24 clone m1 69, by BioLegend (1 mentions) Cd90.2 clone 30 h12, by BioLegend (1 mentions) Nk1.1 clone pk136, by BioLegend (1 mentions) Mhcii clone m5 114, by BioLegend (1 mentions) Cd19 clone 6d5, by BioLegend (1 mentions) Cd11c clone n418, by BioLegend (1 mentions) Cd11b clone m1 70, by BioLegend (1 mentions) Ly6g clone 1a8, by BioLegend (1 mentions) Ly6c clone hk1, by BioLegend (1 mentions) F4 80 clone bm8, by BioLegend (1 mentions) Cd45 clone 30 f11, by BioLegend (1 mentions)

Spelling variants of this product

RA3-6B2 (31 citations) Clone RA3-6B2 (10 citations) Anti-CD45R/B220 (clone RA3-6B2, (4 citations) CD45R/B220 clone RA3-6B2 (3 citations) Alexa-488 anti-CD45R (B220) (clone RA3–6B2), (2 citations) FITC-rat anti-mouse/human CD45R/B220 (clone RA3–6B2, (2 citations)

2 protocols using cd45r clone ra3 6b2

1

Multiparameter Flow Cytometry Panel

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CD3 clone 17A2 (BioLegend 100237 and 100244), CD4 clone RM4–5 (BioLegend 100545 and 100510), CD4 clone GK13 (BioLegend 100403), CD8 clone 53–6.7 (BioLegend 100734), CD11b clone M1/70 (BioLegend 101257), CD11c clone N418 (BioLegend 117339 and 117338), CD19 clone 6D5 (BioLegend 115522), CD24 clone M1/69 (BioLegend 101822), CD45 clone 30-F11 (BioLegend 103139, 103132, and 103114; eBioscience 56–0451-82), CD45R clone RA3–6B2 (BioLegend 103247, 103246, and 103226), CD69 clone H1.2F3 (eBioscience 25–0691-81), CD90.2 clone 30-H12 (BioLegend 105331), CD103 clone 2E7 (BioLegend 121406 and 121414), F4/80 clone BM8 (BioLegend 123108), Flt3L (R&D Systems AF427), Ly6C clone HK1.4 (BioLegend 128037), Ly6G clone 1A8 (BioLegend 127645), MHC-II clone M5/114.15.2 (BioLegend 707631), NK1.1 clone PK136 (BioLegend 108707, 108720, and 108749), Streptavidin-Brilliant Violet 650 (BioLegend 405231), Streptavidin-APC (eBioscience 17–4317-82). Depleting antibodies: NK1.1 clone PK136 (BioXCell BE0036), and IgG2a isotype control (BioXCell BE0085).
Barry K.C., Hsu J., Broz M.L., Cueto F.J., Binnewies M., Combes A.J., Nelson A.E., Loo K., Kumar R., Rosenblum M.D., Alvarado M.D., Wolf D.M., Bogunovic D., Bhardwaj N., Daud A.I., Ha P.K., Ryan W.R., Pollack J.L., Samad B., Asthana S., Chan V, & Krummel M.F. (2018). A Natural Killer/Dendritic Cell Axis Defines Responsive Tumor Microenvironments in Melanoma. Nature medicine, 24(8), 1178-1191.
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2

BM Hematopoiesis Cell Phenotyping

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BM hematopoiesis was analyzed using the following antibodies (all from BioLegend): CD3e (clone 17A3), CD4 (clone GK1.5), CD8 (clone 53-6.7), CD45R (clone RA3-6B2), Mac-1 (clone M1/70), Gr-1 (clone RB6-8C5), Ter-119 (clone TER-119), Sca-1 (clone D7), CD117 (clone 2B8), CD34 (clone RAM34), CD150 (clone TC15-12F12.2), CD48 (clone HM48-1), CD41 (clone MWReg30), CD16/32 (clone 93), Flk2 (clone A2F10), IL-7R (clone A7R34), CD45.1 (clone A20), and CD45.2 (clone 104). BM niche cells were analyzed using the following antibodies (all from BioLegend, unless otherwise indicated): biotin-conjugated leptin receptor (R&D, catalog BAF497), CD31 (clone 390), CD51 (clone RMV-7), CD45 (clone 30-F11), and Ter119. Streptavidin-APC was used as an indirect labeling reagent for biotinylated antibodies. Dead cells were excluded by DAPI labeling (MilliporeSigma).
Lin Y., Gu Q., Lu S., Pan Z., Yang Z., Li Y., Yang S., Lv Y., Zheng Z., Sun G., Gou F., Xu C., Zhao X., Wang F., Wang C., Yuan S., Xie X., Cao Y., Liu Y., Gu W., Cheng T., Cheng H, & Hu X. (2023). Ruxolitinib improves hematopoietic regeneration by restoring mesenchymal stromal cell function in acute graft-versus-host disease. The Journal of Clinical Investigation, 133(15), e162201.
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