In this study, 0.8 g macromolecule compound P34HB (Bluepha, China) was dissolved in 10 mL HFIP (Merck, Germany) to obtain 8% (w/v) mixed polymer solution for electrospinning. After stirred overnight with a magnetic stirrer, the polymer solution was loaded into a standard syringe attached with a 21 G blunted stainless-steel needle. A high voltage of 15 kV was used and the mixed polymer solution was pumped to form a Taylor pendant drop which was then elongated as a fiber under electric field. A rotating collector (SS-2535, Ucalery) with a speed of 120 rpm was applied and the distance between the roller and needle tip was adjusted at 15 cm to collect the aligned fibers. Soy lecithin (Yuanye, China) solution at a concentration of 10 μg mL−1 (ref. 40 (link)) was prepared by dissolved in absolute alcohol (Sigma-Aldrich, China). The electrospun scaffolds collected on the rotating collector were soaked in Soy lecithin solution for 10 min. Finally, electrospun P34HB fiber scaffolds as well as the scaffolds with lecithin were dried overnight in vacuum to evaporate the residual organic solvent. Hence, in this paper, the pure P34HB scaffolds and electrospun P34HB fiber scaffolds with the lecithin are denoted as P34HB and P34HB/lec.
Soy lecithin
Manufactured by Yuanye Bio-Technology
Sourced in China
Soy lecithin is a natural emulsifier and surfactant derived from soybeans. It is used as a functional ingredient in various applications, including food, cosmetics, and pharmaceuticals.
Automatically generated - may contain errors
Lab products found in correlation
Absolute alcohol, by Merck Group (2 mentions)
3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide, by Merck Group (1 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindotricarbocyanine iodide, by Meilun (1 mentions)
Poloxamer 188, by BASF (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Hoechst 33258, by Solarbio (1 mentions)
Labrafac lipophile wl 1349, by Gattefosse (1 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate dii, by Beyotime (1 mentions)
Cholesterol, by Yuanye Bio-Technology (1 mentions)
Pepsin, by Yuanye Bio-Technology (1 mentions)
Pancreatin, by Yuanye Bio-Technology (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
1 1 1 2 2 2 hexafluoro 2 propanol hfip, by Merck Group (1 mentions)
Fitc phalloidin, by Solarbio (1 mentions)
Sprague dawley rat, by Charles River Laboratories (1 mentions)
Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions)
Alizarin red staining kit, by Solarbio (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
Cck 8 kit, by Solarbio (1 mentions)
Alp activity kit, by Nanjing Jiancheng (1 mentions)
5 protocols using soy lecithin
1
Electrospinning of P34HB Scaffolds with Lecithin
2
Formulation and Characterization of Cepharanthine Nanoparticles
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Cepharanthine was purchased from Saen Chemical Technology Co., Ltd. (Shanghai, China). Labrafac lipophile WL 1349 was supplied by Gattefosse (Lyon, France). Soy Lecithin and coumarin-6(Cou6) were purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Poloxamer188 was supplied by BASF (Ludwigshafen, Germany). 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide (DiR) was purchased from Dalian Meilun Biotechnology Co., Ltd. (Dalian, China). Hoechst 33258 and all kinds of enzyme-linked immunosorbent assay (ELISA) kits were purchased from Solarbio (Beijing, China). 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Beyotime Institute of Biotechnology Co., Ltd. (Jiangsu, China). 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) was purchased from Sigma-Aldrich (Louis, USA).
3
Extraction and Characterization of Red Pitaya
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Red pitaya fruits were purchased from a local market in Guangzhou. Soy lecithin and cholesterol were of analytical grade and purchased from Yuanye Biotech Co., Ltd. (Shanghai, China). Sephadex G50 was obtained from Yika Biotech Co., Ltd. (Shanghai, China). DPPH was obtained from Sigma Chemical Company (St. Louis, MO, USA). Pepsin (10,000 u/mg), pancreatin (250 USPu/mg), and bile salts from pig were purchased from Yuanye Biotech Co., Ltd. (Shanghai, China). All other reagents used were analytical grade.
4
Synthesis and Characterization of P34HB Biopolymer
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P34HB (Mw: 2.95 × 105, 89% 3HB and 11% 4HB) was ordered from Blue PHA (Beijing, China). 1,1,1,2,2,2-hexafluoro-2-propanol (HFIP) and absolute alcohol were purchased from Sigma-Aldrich (Darmstadt, Germany). Soy lecithin was purchased from Yuanye company (Shanghai, China). Fetal bovine serum (FBS), α-minimum essential medium (α-MEM), trypsin, and penicillin/streptomycin (PS) were purchased from Gibco (New York, USA). CCK-8 kit, FITC-phalloidin, DAPI and alizarin red staining kit were purchased from Solarbio (Beijing, China). ALP activity kit was purchased from Jiancheng company (Nanjing, China). The RT-PCR kits were purchased from TRANS company (Beijing, China). Sprague-Dawley (SD) rats were supplied by Charles River company (Beijing, China). All the chemical reagents mentioned above were of analytical grade and were used as received without further purification.
5
Ginkgo biloba Leaves Antioxidant Extraction
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Ginkgo biloba leaves picked from the campus of Chongqing Normal University were washed and dried in an oven at 60 ℃ and then ground into powder for use. Petroleum ether, n-butanol, chloroform, chloroacetic acid, isopropyl alcohol, acetic anhydride, ethanol, salicylic acid, H2O2, FeSO4, thiobarbituric acid, trichloroacetic acid were obtained from Chengdu Cologne Chemical Co., Ltd. (Chengdou, China). Concentrated sulfuric acid, concentrated hydrochloric acid and NaOH were obtained from Chuandong Chemical Co., Ltd. (Chengdou, China). Soy lecithin was obtained from Shanghai yuanye Bio-Technology Co., Ltd .(Shanghai, China). DPPH was obtained from RHAWN Co., Ltd. (Shanghai, China). All other relevant reagents were of analytical purity grade.
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