Glucose tolerance testing was conducted as previously described 34 (link). Three days prior to GTT, body composition was obtained to determine lean body mass. Mice were then habituated to consecutive, daily handling sessions. On the study day, mice were fasted for 6 h from 9am- 3pm. Mice were scruffed to obtain a basal glucose read by tail nick, then injected with 1(DIO) to 2 (chow) mg/kg lean mass dose of glucose in sterile PBS. tail vein bleed at 15, 30, 45, 60, 90, and 120 min following injections Glucose readings were obtained by. Lean mass was determined by NMR body composition scan (mq10 Minispec; Bruker; Billericia, MA). Repeated sampling by tail vein bleeding was done at least 1 week apart to allow for complete recovery from blood loss. Area under the curve (AUC) was calculated by the trapezoidal rule.
Minispec mq10
Manufactured by Bruker
Sourced in United States
The Minispec mq10 is a compact and versatile low-field NMR spectrometer designed for routine analysis and quality control applications. It provides fast, non-destructive measurements of various sample properties, including moisture content, oil/fat content, and other parameters. The Minispec mq10 offers a user-friendly interface and is suitable for a wide range of industries, including food, polymers, and petrochemicals.
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Lab products found in correlation
Axiovision 4, by Zeiss (1 mentions)
Axio observer z1 microscope, by Zeiss (1 mentions)
Cns 2000, by Leco (1 mentions)
Vital hn, by Abbott (1 mentions)
Phenomaster labmaster, by TSE Systems (1 mentions)
Teklad global 16 protein rodent diet, by Inotiv (1 mentions)
Labmaster, by TSE Systems (1 mentions)
19 protocols using minispec mq10
1
Glucose Tolerance Test in Mice
2
Glucose Tolerance Test with Body Composition
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Glucose tolerance testing was performed as previously described. 1 week prior to GTT, body composition was obtained. Mice were then habituated to handling for three consecutive sessions. Following habituation, mice were fasted for 4 hr from 2pm-6pm. A basal glucose reading was obtained and mice were then injected with a 2 mg/kg lean mass dose of glucose in PBS. Glucose readings were then obtained at 15, 30,60, and 120 min following injection. Mouse body composition including lean and fat mass was obtained by NMR (mq10 Minispec; Bruker; Billericia, Massachusetts)
3
Longitudinal Growth and Body Composition
4
Norad and Pum2 Regulation of Adiposity
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Whole-body fat, as a percentage of body weight, was measured by nuclear magnetic resonance (NMR) in 3-month and 12-month-old Norad+/+ and Norad–/– mice, or in 4–6 month-old Pum2; rtTA3 double transgenic mice and control littermates after 1.5–2 months of dox treatment, using a Bruker Minispec mq10. Subcutaneous (s.c.) adipose thickness of 12-month-old Norad+/+ and Norad–/– mice was determined using standard H and E-stained skin histology. For every skin sample, images were acquired at 5X magnification across the entire length of the section on an AxioObserver Z1 microscope (Zeiss) using the AxioVision 4.8 software (Zeiss). In these images, the thickness of the s.c. adipose tissue was measured at 15 different points using the AxioVision 4.8 software. The average of the 15 measurements was then calculated to obtain the adipose thickness of each mouse.
5
Olive Tree Spacing Impact on Yield
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Olives were harvested from three individual trees per intra-row spacing on 19/10/2010, 28/10/2011, 17/10/2012, 7/11/2013, 19/10/2015, and 2/11/2016. Due to climatic conditions there was no production in 2014. Subsamples of 25 g were weighed fresh and maturity index (MI) was determined from color of skin and pulp (Uceda and Frías, 1975 ). In 2010 and 2011, three subsamples per tree of 25 g were dried in a forced-air oven at 105°C for 42 h for determination of fruit dry weight. In 2012 and 2013 and in 2015 and 2016, 6 and 15 subsamples, respectively, of 25 g were measured. Fruit oil content was measured in dry subsamples by nuclear magnetic resonance (MiniSpec MQ-10; Bruker, Madison, WI, USA) using the method described by del Río and Romero (1999 ). The number of fruits per tree was estimated from total fresh fruit yield and average fruit fresh weight. Oil production was calculated as the product of fruit number and fruit oil content. Orchard productivity (per ha) was calculated according to tree density.
The effects of intra-row spacing on measured parameters were compared using a randomized block complete design with three replicates. The means were separated using the LSD-test for a level of significance P ≤ 0.05. Regression analysis was used to determine association among parameters.
The effects of intra-row spacing on measured parameters were compared using a randomized block complete design with three replicates. The means were separated using the LSD-test for a level of significance P ≤ 0.05. Regression analysis was used to determine association among parameters.
6
Rectal Temperature and Energy Homeostasis
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We recorded rectal temperatures in fasting and refed mice as described above. For measurements of energy homeostasis, mice were acclimated to the new environment for 3 days before data collection. Measurements of body composition, including fat mass, lean tissue mass, free water, and total body water, were performed in nonanesthetized mice by NMR (Bruker Minispec MQ10).
7
Evaluating Crop Biomass and Nutrient Content
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At harvest, plants were sampled in two areas per subplot. In each sampling area, plants were harvested manually from a given area as a function of the density of the species grown (2.4 m 2 for durum wheat and cereal-legume mixtures and 3 m 2 for sunflower, faba bean, and the sunflower-soybean mixture). Aboveground biomass samples were divided into grain and vegetative parts and, in species mixtures, separated by species. The samples were dried at 80 °C for 48 h. N content in the grain and vegetative parts were determined using the Dumas combustion method with an elemental analyzer (LECO CNS-2000, LECO Corp., St. Joseph, Michigan, USA) from (i) a 10 × 20 cm area for cereals, winter pea, and faba bean and (ii) 20 plants for sunflower, soybean, and sorghum. The content of sunflower seed oil was measured from 125 achenes by nuclear magnetic resonance using a spectrometric analyzer (Minispec mq10, Bruker).
8
High-Fat Diet-Induced Obesity and RYGB in Mice
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Male C57BL/6 mice were started on HFD between 4 and 5 weeks of age. After 12–15 weeks of HFD feeding, mice (approximately 45 g in body weight) were randomized to either RYGB- or sham-operated groups. Mice were allowed to recover under previously described post-operative care [79] (link) during which a liquid diet (Vital HN; Abbott Laboratories, Abbott Park, IL) was provided on post-surgery days 2 through 7. On post-surgery days 6 and 7, 0.25 g HFD was reintroduced and on post-surgery day 8, HFD was provided ad libitum. Seven days post-surgery, a subset of sham-operated mice was calorically restricted and weight matched to RYGB counterparts (weight-matched sham; WMS). All RYGB-operated and the remaining sham-operated mice (ad libitum sham; ALS) were provided HFD ad libitum starting post-surgery day 8. All mice were weighed at 7, 11, 14, 21, 28 and 35 days post-surgery (Figure 3 A). At 5 weeks post-surgery, food intake was measured on 5 consecutive days and body composition was evaluated using a Minispec mq10 nuclear magnetic resonance analyzer (NMR; Bruker Optics, Billerica, MA). At 6 weeks post-surgery, the mice were either perfused and formalin fixed for immunohistochemistry or euthanized for isolation of gastric mucosal cells to establish primary cultures. All mice in these studies were euthanized after an overnight fast (6 PM–10 AM).
9
Comprehensive Metabolic Profiling in Mice
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Mice were monitored for energy expenditure, oxygen consumption and carbon dioxide production, respiratory exchanged rate (RER; V⋅CO2/V⋅O2), food intake and spontaneous locomotor activity using metabolic cages (PhenoMaster/LabMaster, TSE Systems). Mice were individually housed and acclimated to the chambers for 48 h before experimental measurements. In the chambers, food and water consumption was measured automatically. All food intake is reported in kilocalories per day or kilocalories per 12-h dark or light period. Locomotor activity was recorded using infrared light beam-based locomotion monitoring system (beam breaks/h). Data analysis was carried out with Excel XP (Microsoft France, Issy-Les-Moulineaux, France) using extracted raw values of V⋅O2, V⋅CO2 (in ml/h) and energy expenditure (kJ/h). Subsequently, each value was expressed either per total body weight or whole lean tissue mass extracted from the Bruker Minispec mq10 NMR analysis.
10
Comprehensive Mouse Metabolic Phenotyping
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Body weight and composition was determined weekly using quantitative magnetic resonance (QMR) (Bruker's Minispec MQ10, Housten Texas, USA) as described [3] (link). Energy expenditure of single mice was measured by indirect calorimetry over 24 h using an open respirometric system at 11 week of age as described before [2] (link). Mice were kept in normal housing cages and had free access to food and water during the measurement. Locomotor activity was assessed by parallel measurement of spontaneous physical activity using an infrared method and running wheel activity providing mice continuous voluntary access to a running wheel counting wheel rotations (both TSE Systems GmbH, Germany) in the same cages.
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