To generate the OC, cell cultures of adherent PBMCs were supplemented with OC differentiation cytokines (10 ng/mL recombinant human macrophage-colony stimulating factor (hM-CSF) and 10 ng/mL recombinant human receptor activator of NF-κB ligand (hRANKL) (ReproTech, Rocky Hill, NJ, USA)).
Ficoll gradient centrifugation
Ficoll gradient centrifugation is a laboratory technique used for the separation and purification of specific cell types from complex biological samples, such as blood or tissue. It utilizes a density gradient medium, Ficoll, to facilitate the separation of cells based on their density differences during centrifugation. The core function of this technique is to isolate and concentrate specific cell populations for further analysis or downstream applications.
Lab products found in correlation
7 protocols using ficoll gradient centrifugation
Monocyte-Derived Osteoclast Generation
To generate the OC, cell cultures of adherent PBMCs were supplemented with OC differentiation cytokines (10 ng/mL recombinant human macrophage-colony stimulating factor (hM-CSF) and 10 ng/mL recombinant human receptor activator of NF-κB ligand (hRANKL) (ReproTech, Rocky Hill, NJ, USA)).
Isolation and Characterization of Mononuclear Phagocytes
Isolation of Peripheral Blood Mononuclear Cells
FACS Analysis of PBMC Stem Cells
PBMC Proliferation Assay with CSMSCs
Monocyte Isolation and Differentiation
Measuring MSC Immunomodulatory Capacity
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