For Western blot analysis, total protein extract was isolated from tissue samples by RNA/DNA/Protein Purification Kit (Norgen, CA) as described by manufacturer's instructions. Equivalent amounts of protein extract were separated by electrophoresis on 12% SDS-PAGE gels and blotted onto nitrocellulose. The membranes were blocked with 5% nonfat dry milk and 0.1% Tween-20 in Phosphate-buffered saline and then incubated with antibodies followed by appropriate horseradish peroxidase-conjugated secondary antibody (Promega, USA). Rabbit polyclonal anti-LMTK3 (Abcam, UK) was used diluted 1:500, rabbit polyclonal anti-TGFBI (Thermo Scientific, USA) was diluted 1:1000, rabbit polyclonal anti-SOCS2 (Thermo Scientific, USA) was diluted 1:200. Rabbit polyclonal anti-β-actin (Sigma, USA) diluted 1:400 was used to reveal β-actin as a loading control.
Anti socs2
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-SOCS2 is a laboratory equipment product that functions as an antibody targeting the SOCS2 protein. SOCS2 is a member of the suppressor of cytokine signaling (SOCS) family and plays a role in the regulation of cytokine signaling pathways. The Anti-SOCS2 product can be used in various research applications involving the analysis and study of SOCS2 and its associated cellular processes.
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Lab products found in correlation
Horseradish peroxidase conjugated secondary antibody, by Promega (1 mentions)
Anti tgfbi, by Thermo Fisher Scientific (1 mentions)
Rna dna protein purification kit, by Norgen Biotek (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Anti stat3, by Thermo Fisher Scientific (1 mentions)
Bca kit, by Bio-Rad (1 mentions)
Anti abcb1, by Thermo Fisher Scientific (1 mentions)
Anti p stat3, by Thermo Fisher Scientific (1 mentions)
Anti jak2, by Thermo Fisher Scientific (1 mentions)
Anti gapdh, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti socs2
1
Western Blot Analysis of Protein Biomarkers
2
Western Blot Analysis of Protein Expression
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The cells transfected for 48 hours were cleaved using RIRP Lysis Buffer (Thermo Fisher Scientific). Then, the supernatant was collected after centrifugation 4 times at 14 000 rpm for 15 minutes. The protein concentration was detected by bicinchoninic acid (BCA) kit (Bio-Rad, USA). The target protein was separated by SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and transferred into PVDF (polyvinylidene difluoride) membrane overnight. After the blots were sealed using 10% skim milk, the primary antibody was used to hybridize with the target protein overnight at 4°C (anti-MRP1, 1: 1000; anti-GST-π, 1: 2000; anti-ABCB1, 1: 1000; anti-GAPDH, 1: 5000; anti-SOCS2, 1: 1000; anti-SOCS4, 1: 1000; anti-SOCS5, 1: 1000; anti-p-JAK2, 1: 2000; anti-JAK2, 1: 2000; anti-p-STAT3, 1: 2000; anti-STAT3, 1: 2000; Thermo Fisher Scientific). Subsequently, the blots were incubated with horseradish peroxidase conjugated secondary antibody (1: 10 000) after washing in TBST. We used an ECL (enhanced chemiluminescence) kit (Abcam, USA) developed the blots.
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