The TLR7 antibody was purchased from Imgenex Corp., (San Diego, CA, USA), the TLR8 antibody was provided by ProSci Inc. (Poway, CA, USA). COX-2 antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and CD34 antibody from Serotec (Duesseldorf, Germany). Isotype control antibodies were purchased by eBioscience (San Diego, CA, USA). Secondary antibodies were Cy3-conjugated AffiniPure Donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories Inc., Suffolk, UK) and Cy5-conjugated AffiniPure Donkey anti-mouse IgG. The staining was performed on serial cryostat sections of the snap-frozen specimens of pancreatic cancers (UICC II and III) with neighbouring normal pancreas (tumor border) and compared with sections from chronic pancreatitis and normal pancreas. For nuclear counterstaining slides were treated with DAPI (4′,6-Diamidino-2-phenylindoledihydrochlorid) (Sigma-Aldrich, Steinheim, Germany) or haemalaun (Sigma-Aldrich).
Cy5 conjugated affinipure donkey anti mouse igg
Manufactured by Jackson ImmunoResearch
Sourced in United States
Cy5-conjugated AffiniPure donkey anti-mouse IgG is a secondary antibody used to detect and visualize mouse immunoglobulin G (IgG) in various immunological techniques. The antibody is conjugated to the fluorescent dye Cy5, which allows for the detection and quantification of mouse IgG targets.
Automatically generated - may contain errors
Lab products found in correlation
Cy3 conjugated affinipure donkey anti rabbit igg, by Jackson ImmunoResearch (2 mentions)
Isotype control antibody, by Thermo Fisher Scientific (1 mentions)
Tlr7 antibody, by Novus Biologicals (1 mentions)
Cox 2 antibody, by Santa Cruz Biotechnology (1 mentions)
Tcs sp5 confocal laser microscope, by Leica (1 mentions)
Fitc conjugated affinipure donkey anti goat igg, by Jackson ImmunoResearch (1 mentions)
Caseviewer, by 3DHISTECH (1 mentions)
Polyvinylidene fluoride membrane, by GE Healthcare (1 mentions)
Sc 53564, by Santa Cruz Biotechnology (1 mentions)
Ab22656, by Abcam (1 mentions)
4 protocols using cy5 conjugated affinipure donkey anti mouse igg
1
Immunohistochemical analysis of pancreatic cancer
2
Immunofluorescent Imaging of Ileum Tissue
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7 μM paraffin-embedded sections of mouse or human ileum were dehydrated in xylene and ethyl alcohol gradient, incubated in 10 mM sodium citrate for antigen epitope retrieval, blocked in 5% normal donkey serum, and exposed to the following primary antibodies at 4 °C overnight: PHB1 (anti-mouse; 1:500; MS-261-P1, Neomarkers), NIX (anti-rabbit; 1:1000; 12396, Cell Signaling), and CoxIV (anti-goat; 1ug/ul; LSB3256, LSBio). After washing in 1 × PBS, sections were incubated with secondary antibodies (Cy5-conjugated AffiniPure donkey anti-mouse IgG (715-175-150, Jackson ImmunoResearch), FITC-conjugated AffiniPure F(ab’)2 Fragment donkey anti-rabbit IgG (711-096-152, Jackson ImmunoResearch), and Cy3-conjugated AffiniPure donkey anti-goat IgG (705-165-003), Jackson ImmunoResarch). Sections were then stained with 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI, D9542, Sigma) at 1:1000 in 1× PBS for 1 min. Sections were visualized using Zeiss Marianas 3i confocal microscope.
3
Midbrain Immunohistochemistry for Kv7.4 and Kir3.2
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Processes of immunohistochemistry were performed as previously described (Li et al., 2017 (link)), with some modifications. The DAPI-stained coronal midbrain sections (200 μm-thick) from mice that were injected with retrobeads were reconstructed with CaseViewer (Pannoramic MIDI, 3DHISTECH, Hungary). Primary antibodies: mouse anti-TH (tyrosine hydrolyze; 1:400, Merck Millipore, Darmstadt, Germany), rabbit anti-Kv7.4 (1:100, AlomoneLabs, Jerusalem, Israel), goat anti-Kir3.2 (1:400, Santa Cruz Biotechnology, Santa Cruz, CA, USA). Following secondary antibodies were used: FITC-conjugated AffiniPure donkey anti-goat IgG (Jackson ImmunoResearch, West Grove, PA, USA; 1:400), Cy3-conjugated AffiniPure donkey anti-rabbit IgG (Jackson ImmunoResearch, West Grove, PA, USA; 1:400) and Cy5-conjugated AffiniPure donkey anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA; 1:400). Images were obtained on a Leica TCS SP5 confocal laser microscope (Leica, Germany).
4
Western Blot Analysis of Signaling Proteins
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Cells were lysed with RIPA buffer. Protein samples were prepared in 5X loading dye and separated by SDS gel electrophoresis by running on 10% acrylamide‐bisacrylamide gel. Proteins were transferred to polyvinylidene fluoride membrane (GE Healthcare Life science, IL, USA) and membrane was blocked in 5% milk powder for 45 min at RT. Next, membrane blot was incubated overnight at 4 °C with the following primary antibodies: rabbit anti‐phospho‐β catenin (Ser675, 1 : 1000, D2F1, Cell Signaling Technology, MA, USA), mouse anti‐β‐catenin (1 : 1000, 12F7, ab22656, Abcam, MA, USA) and mouse anti‐caveolin (1 : 500, 7C8): sc‐53564, Santa Cruz Biotechnology Inc., TX, USA), rabbit anti‐β‐Actin (1 : 4000; 8457S, Cell Signaling Technology, MA, USA). The next day, blot was washed with TBST three times and incubated with the secondary antibodies goat anti‐rabbit IgG (H + L), DyLight™ 800 4X PEG (1 : 2500, SA5‐35571, Thermo Fischer, MA, USA) or Cy5‐conjugated AffiniPure donkey anti‐mouse IgG (H + L) (1 : 2500, 715‐175‐150, Jackson ImmunoResearch, PA, USA).
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