Dev9911

Manufactured by Demeditec Diagnostics

Sourced in Germany

The DEV9911 is a laboratory equipment product manufactured by Demeditec Diagnostics. It is a device designed for laboratory use, but no further details about its core function can be provided without the risk of making unwarranted interpretations or extrapolations.

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Lab products found in correlation

Es180s 100, by Calbiotech (1 mentions) M2200, by R&D Systems (1 mentions)

7 protocols using dev9911

Measurement of Murine LH and Testosterone Levels

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LH levels were determined by a sandwich ELISA, using a well-established ELISA method (Steyn et al., 2013 (link); Tata et al., 2018 (link)). A mouse LH-RP reference was provided by Albert F. Parlow (National Hormone and Pituitary Program, Torrance, California, USA). The assay sensitivity of the LH ELISA was 0.04 ng/ml and intra-assay coefficient of variation was 4.3%. Plasma T levels were analyzed using a commercial ELISA (Demeditec Diagnostics, GmnH, DEV9911) according to the manufacturers’ instructions as previously validated in other studies (Moore et al., 2015 (link); Tata et al., 2018 (link)). The assay sensitivity is 0.066 ng/ml at the 2 standard deviation confidence limit. Intra-assay coefficient of variation for testosterone was 7 % and inter-assay coefficient of variation was 11.0%. 20 μl of plasma was used for the testosterone assay, run in duplicate samples.
As T and LH levels can vary significantly due to handling, sampling techniques and depending on the time of the day, female adult mice were habituated with daily handling for 3 weeks. Blood samples were taken from trunk blood, for T measurements, and from the tail, for LH measurements, between 10h00 and 12h00 during diestrus.

Mimouni N.E., Paiva I., Barbotin A.L., Timzoura F.E., Plassard D., Le Gras S., Ternier G., Pigny P., Catteau-Jonard S., Simon V., Prevot V., Boutillier A.L, & Giacobini P. (2021). Polycystic ovary syndrome is transmitted via a transgenerational epigenetic process. Cell Metabolism, 33(3), 513-530.e8.

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Hormonal Assays for Mouse Studies

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LH levels were determined by a sandwich ELISA, as described previously74 (link), using the mouse LH–RP reference provided by A.F. Parlow (National Hormone and Pituitary Program, Torrance, CA). Plasma T levels were analyzed using a commercial ELISA (Demeditec Diagnostics, GmnH, DEV9911) 76 (link) according to the manufacturers’ instructions.

Tata B., El Houda Mimouni N., Barbotin A.L., Malone S.A., Loyens A., Pigny P., Dewailly D., Catteau-Jonard S., Sundström-Poromaa I., Piltonen T.T., Dal Bello F., Medana C., Prevot V., Clasadonte J, & Giacobini P. (2018). Elevated prenatal anti-Müllerian hormone reprograms the fetus and induces polycystic ovary syndrome in adulthood. Nature medicine, 24(6), 834-846.

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Mouse Plasma Hormone Measurement

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Immediately after culling, blood was collected from mice by cardiac puncture with a syringe and needle pre-treated with heparin. Plasma was separated by centrifugation and stored at −80 °C. Testosterone was measured using an ELISA kit (DEV9911; Demeditec Diagnostics, Kiel, Germany). LH was measured using an in-house designed ELISA as previously described^{54 (link)}. Samples for each treatment group or age group were analysed together.

Patel S.H., O’Hara L., Atanassova N., Smith S.E., Curley M.K., Rebourcet D., Darbey A.L., Gannon A.L., Sharpe R.M, & Smith L.B. (2017). Low-dose tamoxifen treatment in juvenile males has long-term adverse effects on the reproductive system: implications for inducible transgenics. Scientific Reports, 7, 8991.

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Hormone Measurement Assays in Mice

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T was measured using an ELISA kit (DEV9911; Demeditec Diagnostics, Kiel, Germany) according to the manufacturer’s instructions. Estradiol was measured using a mouse/rat estradiol ELISA kit (ES180S-100; Calbiotech, Spring Valley, CA, USA), which has been shown to provide good correlation to GC-MSMS-derived values (43 (link)) according to the manufacturer’s instructions. Progesterone was measured using a mouse progesterone ELISA kit (CSB-E05104m; Cusabio Biotech Company, Hubei Province, People’s Republic of China) according to the manufacturer’s instructions. LH and FSH were measured using in-house-designed ELISAs as previously described (44 (link)). All samples were run in a single assay for each hormone.

O’Hara L., McInnes K., Simitsidellis I., Morgan S., Atanassova N., Slowikowska-Hilczer J., Kula K., Szarras-Czapnik M., Milne L., Mitchell R.T, & Smith L.B. (2014). Autocrine androgen action is essential for Leydig cell maturation and function, and protects against late-onset Leydig cell apoptosis in both mice and men. The FASEB Journal, 29(3), 894-910.

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Hormonal Assays for Mouse Studies

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Circadian Analysis of Hormones

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Blood sampling for both LH and testosterone measurements was collected over the dark phase 3 h after the lights were turned off. Blood LH levels were determined using a well-established enzyme-linked immunosorbent assay (ELISA) method (74 (link)) (SI Appendix). Plasma testosterone levels were measured in duplicates using a commercial ELISA kit (Demeditec Diagnostics, GmbH, DEV9911) according to the manufacturer’s instructions. The assay sensitivity of this mouse testosterone ELISA was 0.066 ng/mL, and the intra-assay coefficient of variation was 10.4%.

Silva M.S., Decoster L., Trova S., Mimouni N.E., Delli V., Chachlaki K., Yu Q., Boehm U., Prevot V, & Giacobini P. (2022). Female sexual behavior is disrupted in a preclinical mouse model of PCOS via an attenuated hypothalamic nitric oxide pathway. Proceedings of the National Academy of Sciences of the United States of America, 119(30), e2203503119.

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Hormonal and Cytokine Analysis in Mice

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The blood samples were centrifuged at 3,000r.p.m. for 15min at 4°C and stored at −80°C for subsequent serum determinations. The levels of testosterone (DEV9911; Demeditec Diagnostics), estradiol (108667; Abcam), luteinizing hormone (H206–96T; NanJing Jian Cheng Bioengineering Institute), insulin (90080; Crystal Chem) and IL-22 (M2200; R&D Systems) were determined by enzyme-linked immunosorbent assay kits for mice.

Qi X., Yun C., Sun L., Xia J., Wu Q., Wang Y., Wang L., Zhang Y., Liang X., Wang L., Gonzalez F.J., Patterson A.D., Liu H., Mu L., Zhou Z., Zhao Y., Li R., Liu P., Zhong C., Pang Y., Jiang C, & Qiao J. (2019). Gut microbiota–bile acid–interleukin-22 axis orchestrates polycystic ovary syndrome. Nature medicine, 25(8), 1225-1233.

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