Hk205 02

Manufactured by Hycult Biotech

Sourced in Netherlands

The HK205-02 is a lab equipment product offered by Hycult Biotech. It is a tool designed for specific laboratory applications. The core function of this product is to facilitate certain tasks or procedures within a research or testing environment. However, a detailed and unbiased description of its exact capabilities or intended use cannot be provided without the risk of extrapolation. Therefore, the full details of this product are not available in this response.

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Lab products found in correlation

Mcp 1 ccl2, by R&D Systems (1 mentions) Freestyle freedom light, by Abbott (1 mentions) Kma0021, by Thermo Fisher Scientific (1 mentions) Cholesterol quantitation kit, by Merck Group (1 mentions)

Close spelling variants of this product

HK205

2 protocols using hk205 02

Plasma Biomarkers in Fasting Mice

Cited 2 times

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Fasting (5 h) blood samples were taken from the tail vein to prepare EDTA plasma. Blood glucose was determined during blood sampling using a hand-held glucometer (Freestyle Freedom Light, Abbott Laboratories, Lake Bluff, IL, United States). Plasma insulin, leptin, adiponectin, cholesterol, triacylglycerols, ALT, CK-18M30 were determined as described (16 (link)). ELISA assays were used to measure plasma serum amyloid A (SAA; KMA0021 Thermo Fisher Scientific, Waltman, United States), MCP-1/CCL2 (DY479 R&D, Minneapolis, United States), MIF (DY1978 R&D, Fetuin A (DY1563 R&D) and LPS binding protein (LBP; HK205-02 Hycult Biotech, Uden, Netherlands) according to manufacturer’s protocol.
Lipoprotein profiles were analyzed by first separating lipoproteins into fractions using fast protein liquid chromatography (FPLC) with an AKTA apparatus (Pharmacia, Roosendaal, Netherlands), as previously described (17 (link), 18 (link)). Subsequently, total cholesterol and triacylglycerols were measured in the fractions collected for profiling with enzymatic assays (Roche diagnostics, Basel, CHF) (19 (link)–21 (link)).

Gart E., Salic K., Morrison M.C., Giera M., Attema J., de Ruiter C., Caspers M., Schuren F., Bobeldijk-Pastorova I., Heer M., Qin Y, & Kleemann R. (2022). The Human Milk Oligosaccharide 2′-Fucosyllactose Alleviates Liver Steatosis, ER Stress and Insulin Resistance by Reducing Hepatic Diacylglycerols and Improved Gut Permeability in Obese Ldlr-/-.Leiden Mice. Frontiers in Nutrition, 9, 904740.

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Cholesterol and LBP Quantitation

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Levels of circulating cholesterol were determined using the Cholesterol Quantitation Kit (SIGMA, MAK043) as per the manufacturer’s instructions. Serum LBP levels were determined using a commercial ELISA kit (Hycult Biotech, HK205-02).

Caparrós-Martín J.A., Lareu R.R., Ramsay J.P., Peplies J., Jerry Reen F., Headlam H.A., Ward N.C., Croft K.D., Newsholme P., Hughes J.D, & O’Gara F. (2017). Statin therapy causes gut dysbiosis in mice through a PXR-dependent mechanism. Microbiome, 5, 95.

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