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Stat 3 tyr705

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STAT-3 (Tyr705) is a lab equipment product that detects the phosphorylation of STAT3 at tyrosine 705. STAT3 is a transcription factor that is involved in cellular processes such as cell growth and survival. The phosphorylation of STAT3 at tyrosine 705 is a key regulatory event in the activation of STAT3.

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Lab products found in correlation

Stat3, by Cell Signaling Technology (3 mentions) Anti β actin antibody, by Santa Cruz Biotechnology (2 mentions) Recombinant human gm csf, by Thermo Fisher Scientific (2 mentions) Anti nlrp 3 antibody, by Merck Group (2 mentions) Tofacitinib, by Merck Group (2 mentions) Ifn α, by Merck Group (1 mentions) Stat6, by Cell Signaling Technology (1 mentions) Survivin, by Cell Signaling Technology (1 mentions) Stat5, by Cell Signaling Technology (1 mentions) Stat6tyr641, by Cell Signaling Technology (1 mentions) Ptpn6, by Cell Signaling Technology (1 mentions) C myc, by Cell Signaling Technology (1 mentions) Recombinant human il 2, by Thermo Fisher Scientific (1 mentions) Stat1, by Cell Signaling Technology (1 mentions) Mcl 1, by Cell Signaling Technology (1 mentions) Bcl 2, by Cell Signaling Technology (1 mentions) Il 10, by Thermo Fisher Scientific (1 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions) Stat 1 tyr701, by Cell Signaling Technology (1 mentions) Skim milk, by Merck Group (1 mentions)

6 protocols using stat 3 tyr705

1

STAT Signaling Pathway Antibodies

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Phospho-specific antibodies to STAT1Tyr701, STAT3Tyr705, STAT5Tyr694 and STAT6Tyr641 were from Cell Signaling Technologies (Beverly, MA, USA). STAT1, STAT3, STAT5, STAT6, JAK1, JAK2, JAK3, PTPN6, Bcl-2, c-Myc, Mcl-1 and survivin antibodies were also from Cell Signaling Technologies. Actin antibody was purchased from Santa Cruz (Santa Cruz, CA, USA). Recombinant human IL-2, IL-6 and IL-10 were from Peprotech (Rocky Hill, NJ, USA). IFN-α was purchased from Sigma Aldrich (St. Louis, MO, USA).
Demosthenous C., Han J.J., Hu G., Stenson M, & Gupta M. (2015). Loss of function mutations in PTPN6 promote STAT3 deregulation via JAK3 kinase in diffuse large B-cell lymphoma. Oncotarget, 6(42), 44703-44713.
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2

Phospho-specific antibodies for JAK-STAT pathway

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Phospho-specific and pan antibodies against JAK-1 (Tyr1022/1023), JAK-2 (Tyr1007/1008), STAT-1 (Tyr701) and STAT-3 (Tyr705) were purchased from Cell Signaling Technology (Beverly, MA).
Migita K., Iwanaga N., Izumi Y., Kawahara C., Kumagai K., Nakamura T., Koga T, & Kawakami A. (2017). TNF-α-induced miR-155 regulates IL-6 signaling in rheumatoid synovial fibroblasts. BMC Research Notes, 10, 403.
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3

Western Blot Analysis of STAT3 Signaling

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Total protein extract was obtained by homogenising TA muscles with TissueRuptor (Qiagen) proteins Lysis Buffer (50 mM Tris-HCI pH 7,5, Sigma; 1M NaCI, Sigma; 625Mm Saccarosio, Sigma; 10% Glicerolo, Sigma; 1% Triton x-100, Sigma) with protease and phosphatase inhibitors: 1mM PMSF, Sigma; 2 g/ml Aprotinin, Sigma;10 g/ml Leupeptin, Sigma; 10 g/ml Pepstatin, Sigma; 1 mM NaF, Sigma; 0,1 mM Na3V04, Sigma). After separation with 8% SDS—PAGE gels, proteins were transferred to nitrocellulose membranes (Trans-Blot® Turbo™, Bio-Rad, 170-4158) and then blocked in a solution of 5% skim milk (Sigma) in TTBS, and incubated ON at 4 °C with primary antibodies. Western blot was performed using antibodies against the following proteins: STAT3Tyr705 (#9131S, Cell Signaling, 1:1000); STAT3 (#4904S, Cell Signaling, 1:1000); GAPDH (#97166S, Cell Signaling, 1:1000). The membranes were then incubated 1h at RT with the corresponding secondary antibodies: goat polyclonal anti-rabbit (1:2000 in T-TBS) or anti-mouse (1:1000 in T-TBS) IgG antibody conjugated to peroxidase (HRP) (Santa Cruz). The signal was detected with a ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA) and the protein expression levels were then quantified by ImageJ® software and normalized on GAPDH.
Madaro L., Passafaro M., Sala D., Etxaniz U., Lugarini F., Proietti D., Alfonsi M.V., Nicoletti C., Gatto S., De Bardi M., Rojas-García R., Giordani L., Marinelli S., Pagliarini V., Sette C., Sacco A, & Puri P.L. (2018). Denervation-activated STAT3-IL6 signaling in fibro-adipogenic progenitors promotes myofibers atrophy and fibrosis. Nature cell biology, 20(8), 917-927.
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4

Evaluating Anticancer Potential of Astaxanthin

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Acrylamide, bovine serum albumin (BSA), bromophenol blue, 7,12-dimethylbenz[a]anthracene (DMBA), hydroxyurea, 2-mercaptoethanol, sodium dodecyl sulphate (SDS) N,N,N′,N′ - tetramethylene diamine (TEMED) and Trizol were purchased from Sigma Chemical Company, St. Louis, MO, USA. Astaxanthin was procured from Bio-Real, Sweden. DMEM-F12 medium, antibiotic solution consisting of penicillin and streptomycin and Alamar blue were from HiMedia Labs, Mumbai, India. Fetal bovine serum of South American origin was from GIBCO, Invitrogen, NY, USA. Power SYBR Green PCR master mix was obtained from Applied Biosystems, California, USA. Antibodies for IL-6, GAPDH, Cyclin D1, PCNA, p21, MMP-2, MMP-9, TIMP-2, RECK, VEGF, VEGFR2, HIF1α, were purchased from Santa Cruz Biotechnology, USA. pJAK-2tyr1007/1008, JAK-2, pSTAT-3tyr705, STAT-3 and histone (H2B) antibodies and BrdU, STAT-3tyr705, total Cyclin D1 and pVEGFR2tyr1175 ELISA kits were from Cell Signaling Technology, USA. CD-34 antibody was purchased from Novocastra, Germany. Matrigel was from BD Biosciences, USA. All other reagents used were of analytical grade.
Kowshik J., Baba A.B., Giri H., Deepak Reddy G., Dixit M, & Nagini S. (2014). Astaxanthin Inhibits JAK/STAT-3 Signaling to Abrogate Cell Proliferation, Invasion and Angiogenesis in a Hamster Model of Oral Cancer. PLoS ONE, 9(10), e109114.
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5

Cytokine-Induced Inflammasome Activation

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Recombinant human GM-CSF and tumor necrosis factor-alpha (TNF-α) were purchased from PeproTech (Rocky Hill, NJ, USA). Anti-β-actin antibodies were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Anti-NLRP3 antibody was purchased from Merck Millipore (Billerica, MA, USA). Human IL-1β and caspase-1 (p20) enzyme-linked immunosorbent assay (ELISA) kits were purchased from R&D Systems (Minneapolis, MN, USA). Human IL-18 ELISA kits were purchased from MBL (Nagoya, Japan). Phospho-specific antibodies against JAK-1 (Tyr1022/1023), JAK-2 (Tyr1007/1008), STAT-5 (Tyr701), and STAT-3 (Tyr705) were purchased from Cell Signaling Technology (Beverly, MA, USA). Phospho-specific antibody against JAK3 (Tyr980) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-phospho-1κB-α (Ser32), anti-phospho-NF-κB p65 (Ser536), anti-IκBα, and anti-NF-κB antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-phospho-specific Asc (Tyr-144) antibody was purchased from ECM Biosciences (Versailles, KY, USA). Anti-caspase-1 antibody (14F468) was purchased from Novus Biologics (Littleton, CO, USA). Tofacitinib was purchased from Sigma-Aldrich (Tokyo, Japan).
Furuya M.Y., Asano T., Sumichika Y., Sato S., Kobayashi H., Watanabe H., Suzuki E., Kozuru H., Yatsuhashi H., Koga T., Ohira H., Sekine H., Kawakami A, & Migita K. (2018). Tofacitinib inhibits granulocyte–macrophage colony-stimulating factor-induced NLRP3 inflammasome activation in human neutrophils. Arthritis Research & Therapy, 20, 196.
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6

JAK-STAT Signaling in Inflammation

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Recombinant human GM-CSF was purchased from Peprotech (Rocky Hills, NJ). Anti-β-actin antibodies were purchased from Santa Cruz Biotechnology Inc. (Dallas, USA). Anti-NLRP-3 antibody was purchased from MERCK MILLIPORE (Billerica, MA USA). Human IL-1β and caspase-1 (p20) ELISA kits were purchased from R&D systems (Minneapolis, USA). Phospho-specific antibodies against JAK-2 (Tyr1007/1008), STAT-5 (Tyr701) and STAT-3 (Tyr705) were purchased from Cell Signaling Technology (Beverly, MA). Tofacitinib, baricinib and upadacitinib were purchased from Sigma-Aldrich (Tokyo, Japan).
Fujita Y., Matsuoka N., Temmoku J., Furuya-Yashiro M., Asano T., Sato S., Matsumoto H., Watanabe H., Kozuru H., Yatsuhashi H., Kawakami A, & Migita K. (2020). JAK inhibitors impair GM-CSF-mediated signaling in innate immune cells. BMC Immunology, 21, 35.
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