Anti enos and anti phospho enos s1177

Anti-VEGFR2, anti-PLCγ, anti-HSP90, anti-Raf-1, anti-cyclin D1, anti-CDK4 and anti-p21 antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-VEGFR2, anti-phospho-VEGFR2 (Y1175), anti-phospho-PLCγ (Y783), anti-phospho-Erk (T202/Y204), anti-Erk, antiphospho-Akt (S473) and anti-Akt antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-eNOS and anti-phospho-eNOS (S1177) antibodies were purchased from BD Biosciences (San Diego, CA, USA). Anti-α-tubulin antibody was purchased from Sigma-Aldrich. Recombinant human VEGF165 and 4,5-diaminofluorescein diacetate (DAF2-DA) and DAPI (4′,6-diamidino-2-phenylindole dihydrochloride hydrate) were purchased from Sigma-Aldrich. DMAG (17-dimethylaminoethylamino-17-demethoxygeldanamycin) and NOC-18 was purchased from Santa Cruz Biotechnology.

Anti-VEGFR2, anti-PLCγ, and anti-EEA1 antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-VEGFR2, anti-phospho-VEGFR2 (Y1175), anti-PECAM, anti-phospho-PLCγ (Y783), anti-phospho-Erk (T202/Y204), anti-Erk, anti-phospho-Akt (S473), and anti-Akt antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-eNOS and anti-phospho-eNOS (S1177) antibodies were purchased from BD Biosciences (San Diego, CA, USA). Anti-α-tubulin, anti-ephrin-B2, and anti-Flag antibodies were purchased from Sigma-Aldrich. Anti-syntenin antibody was purchased from Abnova (Taipei City, Taiwan). Anti-mouse secondary Alexa 488 and anti-rabbit secondary Alexa 546 antibodies were purchased from Molecular Probes (Invitrogen). Recombinant human VEGF165 and 4,5-diaminofluorescein diacetate (DAF2-DA) were purchased from Sigma-Aldrich.