Log In Sign up
The largest database of trusted experimental protocols

Pdhk3

Manufactured by Novus Biologicals
Visit Supplier

PDHK3 is a laboratory reagent used for research purposes. It is an enzyme involved in the regulation of pyruvate dehydrogenase complex activity. The core function of PDHK3 is to phosphorylate and inactivate the pyruvate dehydrogenase complex, which is a key enzyme in cellular metabolism.

Automatically generated - may contain errors

Lab products found in correlation

Hif 1α, by BD (3 mentions) Pdhk4, by Novus Biologicals (3 mentions) Pdhk2, by Novus Biologicals (3 mentions) Gapdh, by Abcam (2 mentions) Pdha1, by Abcam (2 mentions) Chemidoc, by Bio-Rad (1 mentions) Enhanced chemi luminescence (ecl), by Cytiva (1 mentions) Complete mini protease inhibitor cocktail, by Roche (1 mentions) Hif 2α, by Novus Biologicals (1 mentions)

3 protocols using pdhk3

1

PDH Regulatory Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed with RIPA buffer containing protease and phosphatase inhibitors (5 mM sodium fluoride, 2 mM β -glycerophosphate, 1 mM sodium orthovanadate, 1 mM phenylmethylsulfonyl fluoride (PMSF), and complete mini protease inhibitor cocktail (Roche)), 20–50 μg of total proteins electrophoresed and blotted to PVDF, and probed with anti: PDHA1 (1:6000, MitoSciences), pSer293-E1α (1:1000, EMD Chemicals), pSer300-E1α (1:1000, EMD Chemicals), pSer232-E1α (1:3000, EMD Chemicals), PDHK1 (1:4000, Assay Designs), PDHK2 (1:500, Novus), PDHK3 (1:1000, Novus), PDHK4 (1:1000, Novus), PDP1 (1:1000, Sigma Aldrich), HIF1α (1:1500, BD), and GAPDH (abcam). Primary antibodies were detected with fluorochrome-labeled secondary antibodies (Li-Cor) visualized on a Li-Cor Odyssey.
Echeverri Ruiz N.P., Mohan V., Wu J., Scott S., Kreamer M., Benej M., Golias T., Papandreou I, & Denko N.C. (2021). Dynamic regulation of mitochondrial pyruvate metabolism is necessary for orthotopic pancreatic tumor growth. Cancer & Metabolism, 9, 39.
+ Open protocol
+ Expand
2

Protein Phosphorylation and Enzyme Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lysates were generated in RIPA buffer containing protease and phosphatase inhibitors (5 mM sodium fluoride, 2 mM β-glycerophosphate, 1 mM sodium orthovanadate, 1 mM phenylmethylsulfonyl fluoride, and Complete Mini protease inhibitor cocktail (Roche)), 20–50 μg of total proteins electrophoresed and blotted to PVDF. Antibodies used were for total PDH E1α (1:6000, MitoSciences), pSer293-E1α (1:1000, EMD Chemicals), pSer300-E1α (1:1000, EMD Chemicals), pSer232-E1α (1:3000, EMD Chemicals), PDHK1 (1:4000, Assay Designs), PDHK2 (1:500, Novus), PDHK3 (1:1000, Novus), PDHK4 (1:1000, Novus), PDP1 (1:1000, Sigma Aldrich), HIF1α (1:1500, BD), HIF2α (1:1000, Novus), tubulin (1:1000, Santa Cruz). Primary antibodies were detected with HRP secondaries (1:4000, Santa Cruz) and visualized with ECL (Amersham) on film or ChemiDoc (Bio-Rad), or with fluorochrome labelled secondary antibodies (Li-Cor) visualized on a Li-Cor Odyssey.
Golias T., Papandreou I., Sun R., Kumar B., Brown N.V., Swanson B.J., Pai R., Jaitin D., Le Q.T., Teknos T.N, & Denko N.C. (2016). Hypoxic repression of pyruvate dehydrogenase activity is necessary for metabolic reprogramming and growth of model tumours. Scientific Reports, 6, 31146.
+ Open protocol
+ Expand
3

Characterizing PDH Complex Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed with RIPA buffer containing protease and phosphatase inhibitors (5 mM sodium fluoride, 2 mM β -glycerophosphate, 1 mM sodium orthovanadate, 1 mM phenylmethylsulfonyl fluoride (PMSF), and complete mini protease inhibitor cocktail (Roche)), 20-50 μ g of total proteins electrophoresed and blotted to PVDF, and probed with anti: PDHA1 (1:6000, MitoSciences), pSer293-E1α (1:1000, EMD Chemicals), pSer300-E1α (1:1000, EMD Chemicals), pSer232-E1α (1:3000, EMD Chemicals), PDHK1 (1:4000, Assay Designs), PDHK2 (1:500, Novus), PDHK3 (1:1000, Novus), PDHK4 (1:1000, Novus), PDP1 (1:1000, Sigma Aldrich), HIF1α (1:1500, BD), GAPDH (abcam). Primary antibodies were detected with fluorochrome labelled secondary antibodies (Li-Cor) visualized on a Li-Cor Odyssey.
Ruiz N.P.E., Mohan V., Wu J., Scott S., Kreamer M., Benej M., Papandreou I., & Denko N. (2021). Dynamic regulation of mitochondrial pyruvate metabolism Is necessary for orthotopic pancreatic tumor growth.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!