Rabbit anti p16 ink4a

Rabbit anti-ACAT2 (1:1000) (ab131215) and rabbit anti-P21 (1:1000) (ab109520) were purchased from Abcam. Rabbit anti-SETD7 antibody(1:1000) (24840-1-AP), rabbit anti-P16-INK4A(1:1000) (10883-1-AP), rabbit anti-P27 (1:1000)(25614-1-AP), rabbit anti-Cyclin B1 (1:1000)(55004-1-AP), rabbit anti-Cyclin E1(1:1000)(11554-1-AP), mouse anti-Cyclin D1(1:1000)(60186-1-Ig), rabbit anti-MCM2(1:1000)(10513-1-AP), rabbit anti-Cortactin (1:400)(11381-1-AP) and rabbit anti-SMA (1:1000)(14395-1-AP), mouse anti-Vimentin(1:4000)(60330-1-Ig) were purchased from Proteintech. Rabbit anti-Snail2 (1:1000) (121235) was purchased from Brickell Biotech, Inc. Antibodies against YAP1(1:1000) (A1002), TAZ (1:1000) (A23034), TEAD1(1:1000) (A5218) were purchased from AB clonal. Rabbit anti-vinculin (1:1000) (E1E9V), rabbit anti-flag antibody (1:1000) (2272S), rabbit anti-myc antibody (1:1000) (14793S), rabbit anti-p53 (7F5) (1:1000)(2527S), rabbit anti-E-Cadherin (24E10) (1:1000)(3195S), rabbit anti-N-Cadherin (D4R1H) XP®(1:1000)(13116S) and mouse anti-ubiquitin (1:1000) (#3936) were purchased from Cell Signalling Technology.
MG132 (HY-13259), Cycloheximide (CHX) (HY-12320) and Cell Counting Kit-8 (CCK-8, HY-K0301) were purchased from MedChemExpress (Shanghai, NJ, USA).

Astrocyte and neuronal cultures were fixed with 4% PFA in phosphate‐buffered saline (PBS; pH 7.4) for 15 min and nonspecific sites were blocked with 3% bovine serum albumin (BSA; Sigma‐Aldrich), 5% normal goat serum (Sigma‐Aldrich) and 0.2% Triton X‐100 diluted in PBS for 1 h, before incubation with the following antibodies: rabbit anti‐GFAP (1:1000; DAKO Cytomation), rabbit anti‐lamin‐B1 (1:1000; Abcam), rabbit anti‐p16INK4a (1:100; Proteintech), rabbit anti‐iNOS (1:100; Abcam), rabbit anti‐Spinophilin (1:500; Abcam), or mouse anti‐Synaptophysin (1:1000; Millipore) at 4°C overnight. Subsequently, the cells were thoroughly washed with PBS and incubated with secondary antibodies at room temperature (RT) for 2 h. Secondary antibodies were Alexa Fluor 546‐conjugated goat anti‐rabbit IgG or goat anti‐mouse IgG (1:1000; Invitrogen), or Alexa Fluor 488‐conjugated goat anti‐rabbit IgG or goat anti‐mouse IgG (1:300; Invitrogen). Nuclei were counterstained with DAPI (Sigma‐Aldrich), and cells were observed with a TE2000 Nikon microscope.