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Biotin conjugated anti rabbit igg

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Biotin-conjugated anti-rabbit IgG is a laboratory reagent designed for use in various immunoassay techniques. It is a detection antibody that binds to rabbit immunoglobulin G (IgG) and is labeled with biotin, a small molecule that can be used to signal the presence of the bound target.

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Lab products found in correlation

3 3 diaminobenzidine substrate, by Vector Laboratories (2 mentions) Vectastain elite abc kit, by Vector Laboratories (1 mentions) Alexa fluor 488 goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Alexa fluor 568 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Eclipse te2000 e, by Nikon (1 mentions) Nestin, by Thermo Fisher Scientific (1 mentions) Glut3, by Santa Cruz Biotechnology (1 mentions) βiii tubulin, by Merck Group (1 mentions) Glial fibrillary acidic protein (gfap), by Cell Signaling Technology (1 mentions) Eclipse c1 confocal microscope, by Nikon (1 mentions) Cd133, by Miltenyi Biotec (1 mentions) Avidin biotin complex abc, by Vector Laboratories (1 mentions) Super block, by ScyTek Laboratories (1 mentions) Vectastain abc kit, by Vector Laboratories (1 mentions) Proteinase k, by Merck Group (1 mentions) Anti mmp9, by Abcam (1 mentions) Anti mmp13, by Abcam (1 mentions) Anti cathepsin k, by Abcam (1 mentions) Avidin biotin peroxidase detection system, by Vector Laboratories (1 mentions)

Close spelling variants of this product

Biotin-conjugated goat anti-rabbit IgG (16 citations) Biotin-conjugated goat anti-rabbit IgG and avidin–biotin peroxidase complex (8 citations) Biotin-conjugated anti-rabbit IgG antibody (3 citations) Biotin-anti-rabbit (2 citations) Biotin conjugated goat anti-rabbit IgG (BA-1000, (2 citations) Biotin-conjugated goat anti rabbit IgG secondary antibody (2 citations)

6 protocols using biotin conjugated anti rabbit igg

1

Developmental Expression of APLP2 in Retina

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Retinal expression of APLP2 during embryonic and postnatal development was investigated by immunohistochemistry as previously described [18 ]. Serial eye sections were stained with primary rabbit anti-APLP2 antibody and detected with a secondary antibody, a goat polyclonal biotin-conjugated anti-rabbit IgG (1/1000, Vector Laboratories) and visualized using streptavidin and biotinylated horseradish peroxidase complex (sABC) and diaminobenzidine tetrahydrochloride (DAB). The peroxidase-stained sections were viewed with an Aristoplan-microscope (Zeiss) connected to a digital camera.
Dinet V., Ciccotosto G.D., Delaunay K., Borras C., Ranchon-Cole I., Kostic C., Savoldelli M., El Sanharawi M., Jonet L., Pirou C., An N., Abitbol M., Arsenijevic Y., Behar-Cohen F., Cappai R, & Mascarelli F. (2016). Amyloid Precursor-Like Protein 2 deletion-induced retinal synaptopathy related to congenital stationary night blindness: structural, functional and molecular characteristics. Molecular Brain, 9, 64.
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2

Anti-medaka Sox5 Immunohistochemistry

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The anti-medaka Sox5 rabbit antiserum was prepared by using DYASDNENHITQ synthetic peptide as an epitope of antigen (corresponding to the residues 674–685 of 685 amino acid protein, accession number EF577484). Embryos were fixed in 4% PFA/PBS at 4°C overnight. If necessary, samples were treated with 3% H2O2 and 0.5% KOH in PBS to remove residual melanin before blocking. A primary antibody was diluted 1:300 in 5% goat serum/PBS solution. Biotin-conjugated anti-rabbit IgG (VECTOR) was used as secondary antibody at a 1:500 dilution. Signals were detected by using VECTASTAIN ABC Elite kit (VECTOR) with diaminobenzidine (DAB, MUTO PURE CHEMICALS). For double immunohistochemistry, in combination with anti-Sox5 rabbit antiserum, anti-Pax3/7 mouse monoclonal antibody DP311 ([67 (link)], kindly provided by Dr. Nipam H Patel) was used as a primary antibody to detect Pax7 protein (1:200). Secondary antibodies used were Alexa Fluor 488 goat anti-rabbit and Alexa Fluor 568 goat anti-mouse (Molecular Probes, 1:500).
Nagao Y., Takada H., Miyadai M., Adachi T., Seki R., Kamei Y., Hara I., Taniguchi Y., Naruse K., Hibi M., Kelsh R.N, & Hashimoto H. (2018). Distinct interactions of Sox5 and Sox10 in fate specification of pigment cells in medaka and zebrafish. PLoS Genetics, 14(4), e1007260.
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3

Immunohistochemical Analysis of Neural Markers

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For immunohistochemical staining of formalin-fixed paraffin-embedded tissues, antigen retrieval was performed in citrate buffer at pH 6.0 and 95°C for 20 minutes. Sections were blocked then incubated overnight at 4°C in primary antibody integrin αvβ3 (LM609) or β3 (Cell signaling), Glut3 (Santa Cruz Biotechnology), GFAP (Cell Signaling), βIII tubulin (Sigma-Aldrich), Nestin (Fisher Scientific), CD133 (Miltenyi Biotech) followed by biotin-conjugated anti-rabbit IgG and an avidin-biotin peroxidase detection system with 3,3′-diaminobenzidine substrate (Vector Labs) and counterstained with hematoxylin. Double-immunostaining for β3/Glut3 was carried out according to manufacturer recommendations (Vector Labs). A Nikon Eclipse C1 Confocal microscope as well as a Nikon Eclipse TE2000-E were used for imaging.
Cosset É., Ilmjärv S., Dutoit V., Elliott K., von Schalscha T., Camargo M.F., Reiss A., Moroishi T., Seguin L., Gomez G., Moo J.S., Preynat-Seauve O., Krause K.H., Chneiweiss H., Sarkaria J.N., Guan K.L., Dietrich P.Y., Weis S.M., Mischel P.S, & Cheresh D.A. (2017). Glut3 addiction is a druggable vulnerability for a molecularly defined subpopulation of glioblastoma. Cancer cell, 32(6), 856-868.e5.
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4

Immunohistochemical Analysis of Testis Proteins

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Immunohistochemical staining was performed on 5-μm-thick paraffin human testis sections with or without antigen retrieval in sodium citrate, pH 6.0 at 98°C for 10 min. After inhibition of endogenous peroxidase by 0.3% H2O2, sections were blocked with Superblock (ScyTek). Subsequently, sections were incubated with rabbit primary antibody overnight at 4°C. Primary antibodies used were as follows: anti-ELAVL2 (1:500), anti-TTC14 (1:20) and anti-ENOX1 (1:200) (HPA063001, HPA009295 and HPA038355, respectively, all from Atlas Antibodies), anti-RBM28 (1:20) and anti-TDRD10 (1:50) (ab150800 and ab121982, respectively, both from Abcam) and anti-TPH1 (1:50) (OAAB04046, Avivia Systems Biology). After washing, the sections were incubated with biotin-conjugated anti-rabbit IgG (Vector Laboratories) and subsequently with Avidin-Biotin complex (ABC, Vector Laboratories) and visualized using 3,3′-diaminobenzidine (DAB, Alfa Aesar) as a brown substrate and Hematoxylin as counterstain. As negative controls, we used isotype rabbit IgG instead of the primary antibody. Slides were examined using a brightfield microscope (Olympus BX41).
Jan S.Z., Vormer T.L., Jongejan A., Röling M.D., Silber S.J., de Rooij D.G., Hamer G., Repping S, & van Pelt A.M. (2017). Unraveling transcriptome dynamics in human spermatogenesis. Development (Cambridge, England), 144(20), 3659-3673.
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5

Immunohistochemical Analysis of Rat Knee

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The method of immunohistochemical staining for rat knee sample as follow the previous studies [22] (link), [29] (link), [31] (link). Briefly, the sections were deparaffinized and dehydrated by xylene and ethanol. Endogenous peroxidase was quenched by H2O2 (3%), and the antigen were retrieved by proteinase K (20 mM; Sigma, St Louis, MO, USA). The 4% normal horse serum in PBS was used for minimizing the non-specific adsorption. And the sections were incubated with primary anti-bodies, including anti-cathepsin K (1:100; Abcam; catalogue no. ab25916; polyclonal antibody), anti-MMP9 (1:100; Abcam; catalogue no. ab76003; monoclonal antibody) and anti-MMP13 (1:100; Abcam; catalogue no. ab39012; polyclonal antibody) at overnight at 4 °C, respectively. Biotin-conjugated anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA) and avidin-biotin peroxidase in combination with an ABC kit (Vectastain ABC kit; Vector Labs, Burlingame, CA, USA) were used for specific labeling. And we use 3,3′-diaminobenzidine tetrahydrochloride (DAB) to react with sample. Each section was analyzed by microscope and image output system. We acquired the immunoreactive positive cell on 200× magnification in six fields.
Lin Y.Y., Jean Y.H., Lin S.C., Feng C.W., Kuo H.M., Lai Y.C., Kuo T.J., Chen N.F., Lee H.P, & Wen Z.H. (2020). Etoricoxib prevents progression of osteolysis in repeated intra-articular monosodium urate-induced gouty arthritis in rats. Journal of Advanced Research, 24, 109-120.
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6

Immunohistochemical Staining of Tissue Samples

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For immunohistochemical staining of formalin-fixed paraffin-embedded tissues, antigen retrieval was performed in citrate buffer at pH 6.0 and 95°C for 20 min. Sections were blocked in normal goat serum diluted in PBS, incubated overnight at 4°C in primary antibody followed by biotin-conjugated anti-rabbit IgG and an avidin–biotin peroxidase detection system with 3,3′-diaminobenzidine substrate (Vector) then counterstained with hematoxylin. Whole-mount mouse mammary glands were fixed in Carnoy’s solution and stained with carmine. For quantitation of duct/alveoli density, 3–4 images were randomly sampled from H&E-stained paraffin sections from each mouse with a 4× objective and analyzed with Metamorph software. For immunofluorescence, frozen sections or fixed cells were blocked with normal goat serum in PBS and incubated in primary antibody overnight at 4°C followed by secondary at room temperature for 1 h. For further details, see Supplemental Experimental Procedures.
Desgrosellier J.S., Lesperance J., Seguin L., Gozo M., Kato S., Franovic A., Yebra M., Shattil S.J, & Cheresh D.A. (2014). Integrin αvβ3 Drives Slug Activation and Stemness in the Pregnant and Neoplastic Mammary Gland. Developmental cell, 30(3), 295-308.
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