Log In Sign up
The largest database of trusted experimental protocols

Biotin nhs ester

Manufactured by Thermo Fisher Scientific
Visit Supplier

Biotin-NHS ester is a chemical compound used in various biomedical and biotechnology applications. It is a bifunctional reagent that can be used to covalently attach biotin to amine-containing molecules. The NHS (N-hydroxysuccinimide) ester group of Biotin-NHS ester reacts with primary amines, forming a stable amide bond. This allows for the efficient labeling of proteins, peptides, or other biomolecules with biotin, which can then be utilized in downstream applications that involve biotin-streptavidin interactions.

Automatically generated - may contain errors

Lab products found in correlation

Atto565 n hydroxysuccinimide ester nhs, by Merck Group (2 mentions) Atto647n nhs ester, by Merck Group (2 mentions) Alexa fluor 488 nhs ester, by Thermo Fisher Scientific (1 mentions) Mf 319 developer, by MicroChem (1 mentions) Neutravidin protein, by Thermo Fisher Scientific (1 mentions) Alexa fluor 647 nhs ester, by Thermo Fisher Scientific (1 mentions) Sodium oleate, by Tokyo Chemical Industry (1 mentions)

4 protocols using biotin nhs ester

1

Purification and Labeling of Tubulin

Check if the same lab product or an alternative is used in the 5 most similar protocols
Porcine brain tubulin was purified as described66 (link) and for fluorescence microscopy assays labelled with Atto565-N-hydroxysuccinimide ester (NHS; Sigma-Aldrich), or Atto647N-NHS ester (Sigma-Aldrich), or biotin-NHS ester (Thermo Scientific) according to published methods67 (link).
The sequences of all newly cloned constructs were verified by sequencing. All protein purifications were carried out at 4°C. Protein concentrations were determined either using a Bradford protein assay, or for tubulin, by measuring the absorbance at 280 nm. Protein concentrations refer to monomers, except for tubulin concentrations, which refer to dimers.
Roostalu J., Cade N.I, & Surrey T. (2015). Complementary activities of TPX2 and chTOG constitute an efficient importin-regulated microtubule nucleation module. Nature cell biology, 17(11), 1422-1434.
+ Open protocol
+ Expand
2

Antigen Production and Conjugation Protocol

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
RSV prefusion F antigen trimer, EBV gH/gL complex, and a modified HIV Envelope GP140 trimer (426c TM4ΔV1–3) were produced as described (29 (link), 55 (link), 56 (link)). Stabilized influenza HA stem was produced from VRC clone 3925, derived from strain H1 1999 NC as described (57 (link)). All antigens were conjugated to Biotin NHS ester (Thermo Fisher Scientific) followed by tetramerization with streptavidin-PE, streptavidin-APC or streptavidin (all from Prozyme) as described previously (58 (link)). RSV F antigen was conjugated to Alexa Fluor 488 NHS ester (Thermo Fisher Scientific) according to the manufacturer’s recommendations and used for flow cytometry.
Moffett H.F., Harms C.K., Fitzpatrick K.S., Tooley M.R., Boonyaratanakornkit J, & Taylor J.J. (2019). B cells engineered to express pathogen-specific antibodies protect against infection. Science immunology, 4(35), eaax0644.
+ Open protocol
+ Expand
3

Purification and Labeling of Tubulin

Check if the same lab product or an alternative is used in the 5 most similar protocols
Porcine brain tubulin was purified as described66 (link) and for fluorescence microscopy assays labelled with Atto565-N-hydroxysuccinimide ester (NHS; Sigma-Aldrich), or Atto647N-NHS ester (Sigma-Aldrich), or biotin-NHS ester (Thermo Scientific) according to published methods67 (link).
The sequences of all newly cloned constructs were verified by sequencing. All protein purifications were carried out at 4°C. Protein concentrations were determined either using a Bradford protein assay, or for tubulin, by measuring the absorbance at 280 nm. Protein concentrations refer to monomers, except for tubulin concentrations, which refer to dimers.
Roostalu J., Cade N.I, & Surrey T. (2015). Complementary activities of TPX2 and chTOG constitute an efficient importin-regulated microtubule nucleation module. Nature cell biology, 17(11), 1422-1434.
+ Open protocol
+ Expand
4

Synthesis and Functionalization of Iron Oxide Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Iron (III) chloride hexahydrate was purchased from Across (99%) and sodium oleate from TCI America (97%). Neutravidin protein, Alexa Fluor 647-NHS ester and Biotin-NHS ester were purchased from Thermo Fisher Scientific. LOR photoresist and MF-319 developer were obtained from Microchem and S1805 photoresist was obtained from Microposit. Other reagents and chemicals were purchased from Sigma-Aldrich and used as received unless otherwise stated.
Castellanos-Rubio I., Munshi R., Qin Y., Eason D.B., Orue I., Insausti M, & Pralle A. (2018). Multilayered Inorganic-Organic Microdisks as Ideal Carriers for High Magnetothermal Actuation: Assembling Ferromagnetic Nanoparticles Devoid of Dipolar Interactions.. Nanoscale, 10(46), 21879-21892.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!