Anti egfr 1005

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-EGFR (1005) is a laboratory reagent that specifically recognizes the epidermal growth factor receptor (EGFR) protein. It can be used as a tool in various research applications involving EGFR.

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Close spelling variants of this product

Anti-EGFR (81 citations) EGFR (1005) (9 citations) Anti-EGFR ((1005) SC-03 – (4 citations) Anti-EGFR rabbit polyclonal antibody (clone 1005, (2 citations) Polyclonal rabbit anti-EGFR (1005; (2 citations)

7 protocols using anti egfr 1005

Inhibition of Immune Signaling Pathways

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Antibodies used in vitro were anti-EGFR (1005; Santa Cruz), anti-FasL (NOK1; BD Biosciences), anti-TLR2 (H 175; Santa Cruz), anti-TNFR1 (H-271; Santa Cruz) and anti-IL-16 (ab9563; Abcam). Respective IgG isotype controls were used for comparison; rabbit (2027; Santa Cruz) or mouse (2025; Santa Cruz). Inhibitors used were pancaspase inhibitor ZVAD-FMK (Santa Cruz), caspase-3 inhibitor (Calbiochem), caspase 1 inhibitor (Calbiochem), calpeptin (Calbiochem), BAPTA (Calbiochem) and EDTA (Sigma).

Ahn D., Parker D., Planet P.J., Nieto P.A., Bueno S.M, & Prince A. (2014). Secretion of IL-16 through TNFR1 and calpain-caspase signaling contributes to MRSA pneumonia. Mucosal immunology, 7(6), 1366-1374.

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EGFR Immunoprecipitation and Ubiquitination Analysis

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Immunoprecipitation assays were carried out as previously described [26 (link)]. In brief, treated cells were lysed with the RIPA buffer and protein concentrations were measured. One mg of protein per condition was incubated at 4 °C with anti-EGFR (R1, Santa Cruz) antibody and protein G-agarose (Roche) for 4 h. Beads were then washed 3 times with the YP-IP buffer (10 mM Tris-HCl pH 7.5, 0.1% Nonidet P-40, 150 mM NaCl), before immunoprecipitated proteins were eluted with 1.5 X SDS-PAGE loading buffer. Samples were further analyzed by immunoblotting with anti-EGFR (1005, Santa Cruz) and anti-Ubiquitin (P4G7, Covance) antibodies.

Wang T., Zhang J., Wang S., Sun X., Wang D., Gao Y., Zhang Y., Xu L., Wu Y., Wu Y., Liu F., Liu X., Liu S., Zhang Y., Wang Y., Zou L, & Liu H. (2018). The exon 19-deleted EGFR undergoes ubiquitylation-mediated endocytic degradation via dynamin activity-dependent and -independent mechanisms. Cell Communication and Signaling : CCS, 16, 40.

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Characterization of Vangl2 and Wnt Signaling

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HEK293T cells were from ATCC, and T98G, U87-MG, A1207, and U251 cells were gifts from Dr. Paul Knoepfler. T98G, U87-MG and U251 cells were authenticated by Arizona Research Labs (http://uagc.arl.arizona.edu/). Antibodies employed were anti-Vangl2 N-13, anti-Muc4 P-20, anti-Rac1, anti-EGFR (1005) from Santa Cruz, anti-FLAG M2, anti-tubulin, anti-actin AC-15 from Sigma, anti-HA 12CA5 for Ub-HA from Roche, anti-Myc 9E10 from Calbiochem, anti-V5 from Invitrogen, anti-RhoA from BD Biosciences, anti-FLRF/RNF41/Nrdp1 from Bethyl Laboratories, anti-HA C29F4 for Fzd7-HA western blots, anti-Dvl2, anti-phospho-JNK (T183/Y185), anti-phospho-Erk (T202/Y204), anti-phospho-Akt (S473), anti-phospho-β-catenin (Ser33/37/Thr41), anti-phospho-β-catenin (Ser552), and anti-β-catenin from Cell Signaling.

Wald J.H., Hatakeyama J., Printsev I., Cuevas A., Fry W.H., Saldana M.J., Vorst K.V., Rowson-Hodel A., Angelastro J.M., Sweeney C, & Carraway KL I.I.I. (2017). Suppression of planar cell polarity signaling and migration in glioblastoma by Nrdp1-mediated Dvl polyubiquitination. Oncogene, 36(36), 5158-5167.

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Inhibition of Immune Signaling Pathways

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Characterization of Vangl2 and Wnt Signaling

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Antibody Validation for Immunoblotting and IF

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Antibodies for immunoblotting were from commercially available sources. anti-δ-catenin (#611537, BD Bioscience); anti-GFP (#G1544, Sigma); anti-p-Erk1/2 (#16982, Santa Cruz); anti-Erk1 (SC-94, Santa Cruz); anti-EGFR 1005 (#03, Santa Cruz); anti-EGFR (D20) (#31156, Santa Cruz) anti-E-cadherin (SC-7870, Santa Cruz); anti-py20 (SC-508, Santa Cruz) and anti-Myc-Tag (9B11) (#2276,Cell signaling).
Antibodies for immunofluorescence were also obtained commercially: anti-E-cadherin (#3195, Cell signaling), anti-EGFR 225 (MA5-12880, ThermoFisher Scientific).
Control siRNA and siRNA anti-δ-catenin were purchased from Sigma-Aldrich.

He Y., Ryu T., Shrestha N., Yuan T., Kim H., Shrestha H., Cho Y.C., Seo Y.W., Song W.K, & Kim K. (2016). Interaction of EGFR to δ-catenin leads to δ-catenin phosphorylation and enhances EGFR signaling. Scientific Reports, 6, 21207.

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Assaying Prostate Cancer Cell Markers

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Dihydrotestosterone (DHT) and anti-androgen bicalutamide were purchased from Sigma-Aldrich Co., LLC. (St. Louis, MO, USA). Recombinant human EGF, FGF-2, FGF-7, FGF-10, hepatocyte growth factor (HGF), IGF-1, transforming growth factor (TGF) β1, vascular endothelial growth factor (VEGF), and IL-6 were purchased from PeproTech, Inc. (Rocky Hill, NJ, USA). Rabbit polyclonal anti-PSA and mouse monoclonal anti-neuron-specific enolase (NSE; BBS/NC/V1-H14) antibodies were purchased from Dako Cytomation (Copenhagen, Denmark). Rabbit polyclonal anti-AR (N-20) and anti-EGFR (1005) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit polyclonal anti-CD31 and anti-Ki-67 and rabbit monoclonal anti-AR splice variant 7 (AR-V7; EPR15656) antibodies were purchased from Abcam Inc. (Cambridge, MA, USA). Rabbit monoclonal anti-phospho-STAT3 (Tyr705) (D3A7), anti-STAT3 (D3Z2G), anti-phospho-Akt (Ser473) (D9E), and anti-Akt (pan) (C67E7) antibodies were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). Rabbit polyclonal anti-p44/42 MAPK and mouse monoclonal anti-phospho-p44/42 MAPK (Thr202/Tyr204) (E10) antibodies were purchased from Cell Signaling Technology, Inc. Mouse monoclonal anti-β-actin (AC-15) antibodies were purchased from Sigma-Aldrich Co., LLC.

Ishii K., Matsuoka I., Sasaki T., Nishikawa K., Kanda H., Imai H., Hirokawa Y., Iguchi K., Arima K, & Sugimura Y. (2019). Loss of Fibroblast-Dependent Androgen Receptor Activation in Prostate Cancer Cells is Involved in the Mechanism of Acquired Resistance to Castration. Journal of Clinical Medicine, 8(9), 1379.

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