E el m1382c

Manufactured by Elabscience

Sourced in China

The E-EL-M1382c is a laboratory equipment product offered by Elabscience. It serves as a core function, but a detailed description while maintaining an unbiased and factual approach is not available.

Automatically generated - may contain errors

Lab products found in correlation

E el m3012, by Elabscience (2 mentions) E bc k261, by Elabscience (1 mentions) Rk00027, by ABclonal (1 mentions) Rk00008, by ABclonal (1 mentions) Rk00036, by ABclonal (1 mentions)

5 protocols using e el m1382c

Measurement of Insulin Resistance and Triglycerides

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Blood was collected from the heart, and the fasting blood glucose level was measured. The blood samples were left at room temperature for 2 h, and then centrifuged at 1,000 × g for 20 min. The supernatant was collected, and the serum insulin content was detected using an INS ELISA kit (E-EL-M1382c; Elabscience, Wuhan, China). Finally, the homeostatic model assessment for insulin resistance (HOMA-IR) index and the homeostatic model assessment for insulin sensitivity index (HOMA-ISI) of each group were calculated using the following formulae (Fang et al., 2010 (link)): insulin resistance index = [fasting blood glucose (mmol/L) × serum insulin (mIU/L)] / 22.5; insulin sensitivity index = 1 / [fasting blood glucose (mmol/L) × serum insulin (mIU/L)]. Triglycerides (TGs) in the liver tissue or serum were measured using a TG assay kit (E-BC-K261; Elabscience, Wuhan, China).

Zhang D., Ma Y., Liu J., Deng Y., Zhou B., Wen Y., Li M., Wen D., Ying Y., Luo S., Shi C., Pu G., Miao Y., Zou C., Chen Y, & Ma L. (2021). Metformin Alleviates Hepatic Steatosis and Insulin Resistance in a Mouse Model of High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease by Promoting Transcription Factor EB-Dependent Autophagy. Frontiers in Pharmacology, 12, 689111.

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Quantifying Metabolic and Inflammatory Markers

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Plasma insulin (E-EL-M1382c, Elabscience, Wuhan, China), GLP-1 (E-EL-M3012, Elabscience), TNF-α (RK00027, ABclonal, Wuhan, China), IL-6 (RK00008, ABclonal), and IL-4 (RK00036, ABclonal) levels were determined via ELISA according to the manufacture’s protocols. Total cholesterol (A111-1-1), triglyceride (A110-1-1), low-density lipoprotein cholesterol (A113-1-1), and high-density lipoprotein cholesterol (A112-1-1) were measured using relative commercial colorimetric enzymatic assay kits from Najing Jiancheng Bioengineering, as described previously [25 (link)].

Zhou Y., Yuan Z., Wang M., Zhang Z., Tan C., Yu J., Bi Y., Liao X., Zhou X., Ali Sheikh M.S, & Yang D. (2023). Liraglutide Attenuates Aortic Valve Calcification in a High-Cholesterol-Diet-Induced Experimental Calcific Aortic Valve Disease Model in Apolipoprotein E-Deficient Mice. Journal of Cardiovascular Development and Disease, 10(9), 386.

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ELISA Quantification of Mouse Hormones

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ELISA kits for mouse ghrelin (E-EL-M0551c; Elabscience), insulin (E-EL-M1382c, Elabscience), GLP-1 (E-EL-M3012; Elabscience), and PYY (E-EL-M2375c; Elabscience) were purchased from BGsciences Biotechnology Co., Ltd. (Guangzhou, China). ELISA was performed using serum samples. Blood samples were obtained from heart and allowed to clot for 30 min at 37 °C. After centrifugation at 2000g for 15 min, the serum was collected from the blood samples. Serum was used for ELISA. The ELISA was performed according to the manufacturer’s instructions. The standard curves were plotted for each experiment.

Wu Y., Feng X., Li M., Hu Z., Zheng Y., Chen S, & Luo H. (2023). Gut microbiota associated with appetite suppression in high-temperature and high-humidity environments. eBioMedicine, 99, 104918.

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Serum Insulin Measurement in Mice

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After fasted for 6 h, whole blood was collected from the posterior vena cava of mice soon after euthanasia. Serum was then obtained after centrifuging the whole blood at 2000 rpm for 10 min at room temperature. Serum insulin levels were measured using an enzyme-linked immunosorbent assay (ELISA) kit (E-EL-M1382c, Elabscience, China) according to the manufacturer's instructions.

Hua H., Wu M., Wu T., Ji Y., Jin L., Du Y., Zhang Y., Huang S., Zhang A., Ding G., Liu Q, & Jia Z. (2023). Reduction of NADPH oxidase 4 in adipocytes contributes to the anti-obesity effect of dihydroartemisinin. Heliyon, 9(3), e14028.

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Fasting Metabolic Markers in Mice

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All mice fasted for 12 h after prior acupuncture. On the second morning, fasting blood glucose (FBG) levels were tested from mouse tails. Mice were then anesthetized with tribromoethanol and blood samples were collected by eye enucleation. Samples were measured by ELISA to detect fasting insulin (FINS) (E-EL-M1382c; Elabscience, Wuhan, China) and Adipo (E-EL-M0002c; Elabscience, Wuhan, China). ELISA was performed according to the manufacturer’s instructions. Additionally, the following formula was used to calculate the homeostasis model assessment estimated insulin resistance (HOMA-IR) = FINS (mU/L) × FBG (mmol/L)/22.5.

Xu W., Li J., Ji C., Fang D., Yao L., Xu N, & Yi W. (2023). Activation of POMC neurons to adiponectin participating in EA-mediated improvement of high-fat diet IR mice. Frontiers in Neuroscience, 17, 1145079.

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