Each vascular graft tissue sample for SEM was prepared by the Centre for Microscopy at the University of the Free State. All samples were fixed in 2.5% glutaraldehyde (Merck, Johannesburg, South Africa). Tissue specimens were dried using the critical point method (Tousimis critical point dryer, Rockville, MD, USA, ethanol dehydration, and carbon dioxide drying gas) and were metallized using gold (BIO-RAD, Microscience Division Coating System, London, UK; Au/Ar sputter coating @ 50–60 nm). Evaluations were performed with a Shimadzu SSX 550 scanning electron microscope (Kyoto, Japan, with integral imaging). The surface area of each specimen was examined and photographed in different positions. SEM micrographs were used to assess endothelial integrity and to evaluate the quality of the extracellular basal membrane.
Ssx 550 scanning electron microscope
Manufactured by Shimadzu
Sourced in Japan
The SSX-550 is a scanning electron microscope (SEM) manufactured by Shimadzu. It is designed to produce high-resolution images of the surface of solid samples by scanning them with a focused beam of electrons. The SSX-550 provides detailed information about the topography, composition, and other properties of the sample.
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3 protocols using ssx 550 scanning electron microscope
1
Vascular Graft Tissue Preparation for SEM
2
Scanning Electron Microscopy of Yeast Cells
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Yeast S. cerevisiae cell suspensions in (i) water-glycerol at 1.4 MPa (iso-osmotic), (ii) osmotically dehydrated at 30.0 MPa, or (iii) rehydrated (with 1.4 MPa solution) cells were vacuum filtered through a 0.22-μm cellulose acetate membrane. Samples were washed three times with 0.01 M PBS solution (pH 7.4) and fixed with 2 % (v/v) glutaraldehyde in 0.01 M PBS for 60 min at 4 °C. The dehydration was performed by applying ethanol gradients of 30, 50, 70, and 100 % (three cycles) v/v at 4 °C for 5 min each one. After 30 min, cells were freeze-dried. Cell fragments were fixed and coated with gold using a SC-701AT sputter-coater (Sanyu Electron, Tokyo, Japan). Images of cell surface were acquired at 12 kV using a SSX-550 scanning electron microscope (Shimadzu, Kyoto, Japan).
3
Scanning Electron Microscopy of Polymer Blends
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A Shimadzu SSX-550 scanning electron microscope (SEM) operating in a high vacuum chamber at acceleration voltage of 15 kV was used to analyze the surface morphology of the blend and nanocomposite films. A secondary electron detector was used to capture the images. A nanolayer of gold particles was deposited on the film surfaces for 2 min at 330 V and 20 mA in a physical vapor depositor (PVD).
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