Post-challenge time points for blood sampling and rectal temperature measurements varied by study and are listed in the supplementary table legends. The following hematology and clinical chemistry parameters are shown in the supplementary tables: granulocyte counts, levels of alanine aminotransferase, prothrombin time (PT), and activated partial thromboplastin time (aPTT). Granulocyte counts were determined in blood containing EDTA using either a VetScan HM2 Analyzer (Abaxis Inc,) or a COULTER Ac.T 5diff AL (Beckman Coulter Inc.). Clinical chemistry parameters were measured in serum using a VetScan analyzer or Piccolo Xpress (both Abaxis Inc). PT and aPTT were measured in a Coag DX analyzer (IDEXX Laboratories Inc.). Petechial rash was recorded on clinical observation sheets at least twice daily by staff blinded to study treatment.
Vetscan hm2 analyzer
Manufactured by Abaxis
Sourced in United States
The VetScan HM2 Analyzer is a compact, automated hematology analyzer designed for veterinary use. It provides a comprehensive analysis of red blood cells, white blood cells, and platelets in a small blood sample. The device is capable of performing complete blood count (CBC) tests, offering key hematological parameters to aid in the diagnosis and monitoring of various animal health conditions.
Automatically generated - may contain errors
Lab products found in correlation
Piccolo xpress, by Abaxis (1 mentions)
Coag dx analyzer, by IDEXX (1 mentions)
Vet scan analyzer, by Abaxis (1 mentions)
Automatic analyzer model 7180, by Hitachi (1 mentions)
40 μm cell strainer, by SPL Life Sciences (1 mentions)
Newborn calf serum ncs, by Thermo Fisher Scientific (1 mentions)
Rbc lysing buffer, by Merck Group (1 mentions)
Collagenase d, by Merck Group (1 mentions)
Dnase 1, by Merck Group (1 mentions)
Histopaque 1083, by Merck Group (1 mentions)
Collagenase type 1, by Thermo Fisher Scientific (1 mentions)
Dnase 1, by Worthington (1 mentions)
5 protocols using vetscan hm2 analyzer
1
Hematology and Clinical Chemistry Analysis
2
Hematological and Biochemical Analysis
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Blood samples were collected three times on day 0, 27, and 48 of the experiment period at 1400 h from jugular venipuncture into K2 EDTA-treated vacutainers (4 mL; Becton Dickinson, Franklin Lakes, NJ, USA) for measuring hematology (white blood cells, lymphocytes, monocytes, granulocytes, red blood cells, hemoglobin, hematocrit, mean corpuscular hemoglobin, and plateletcrit) using the VetScan HM2 analyzer (Abaxis, Union City, CA, USA). Subsequently, blood in serum tubes was centrifuged at 2500× g for 15 min at 4 °C. The serum was used for measurement of biochemical parameters (glucose, total protein, blood urea nitrogen, albumin, and triglyceride) using the automated biochemical analyzer (Hitachi Automatic Analyzer model 7180, Hitachi, Tokyo, Japan) according to manufacturer’s instructions.
3
Single-cell Isolation from Murine Tissues
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Single‐cell suspensions of BM cells were prepared by flushing the leg bones (one tibia and one femur per mouse) with RPMI‐1640 supplemented with 2% Newborn Calf Serum (NCS; Thermo Fisher Scientific) plus antibiotic‐antimycotic. Single‐cell suspensions of spleens were prepared by dissociating the tissues and filtered through a 40‐μm cell strainer (SPL Life Sciences Co., Ltd. Pocheon‐si, Korea). Peripheral blood was harvested, and complete blood count analysis was performed using VetScan® HM2 analyzer (Abaxis, Inc. Union City, CA, USA). Red blood cells (RBCs) were removed by using RBC lysing buffer (Sigma‐Aldrich, Saint Louis, MO, USA). Peripheral blood mononuclear cells were collected using Histopaque®‐1083 (Sigma‐Aldrich). Tumors were weighed and dissected mechanically and then digested with 400 units mL−1 collagenase D (Sigma‐Aldrich) and 200 μg mL−1 DNase I (Sigma‐Aldrich).
4
Fabrication and Activation of ECM Scaffolds
5
Isolation of Endothelial, MSC, and Osteoblast Cells
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Bone marrow cells were flushed out from femoral and tibial bones and suspended in media. To obtain endothelial cells and MSCs, bone marrow cells were incubated in D-MEM high glucose with 2% FCS, 5 mg/ml collagenase type I (Gibco), and 100 μg/ml DNase I (Worthington Biochemical Corporation) at 37°C for 20 min before cell suspension. To obtain endothelial cells, liver, spleen, lung, and kidney were dissected and incubated for 30 min before cell suspension in the media. To obtain osteoblasts, marrow depleted-bones were dissected and crushed and then incubated for 45 min before cell suspension in the media. The number of white blood cells and hematocrit in peripheral blood was measured using a Vetscan HM2 Analyzer (Abaxis).
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