Lipoproteins were separated from 0.5 ml human plasma by FPLC according to the method described by Innis-Whitehouse and colleagues[14 (link)]. Cholesterol was determined in each fraction by using a cholesterol assay kit (Sigma) following the manufacturer’s protocol. Human plasma (5 μl) or equal volume (25μl) from each FPLC fraction was subjected to SDS PAGE and Western blotting as described above to detect β-COP with two different anti-β-COP monoclonal antibodies (Sigma, the clone MAD at 1:500 or the clone M3A5 at 1:125 dilutions).
Cholesterol assay kit
Manufactured by Merck Group
The Cholesterol assay kit is a laboratory tool designed to measure the concentration of cholesterol in biological samples. It provides a standardized procedure for the quantitative analysis of cholesterol levels.
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Lab products found in correlation
2 protocols using cholesterol assay kit
1
FPLC Separation and Western Blot Analysis of Lipoproteins
2
Cellular Cholesterol Quantification
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The quantitative result of cellular cholesterol content was analysed by Cholesterol Assay Kit (Sigma‐Aldrich) and then measured using a microplate reader.
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