Full field laser perfusion imager

Manufactured by Moor Instruments

Sourced in United Kingdom

The Full-Field Laser Perfusion Imager is a non-invasive device that uses laser speckle imaging technology to measure and visualize blood flow in real-time. It captures high-resolution images of tissue perfusion, providing quantitative data on microvascular function.

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Lab products found in correlation

R statistical analysis software, by R Project for Statistical Computing (1 mentions) Matlab r2013b software, by MathWorks (1 mentions)

3 protocols using full field laser perfusion imager

Laser Speckle Imaging of Flap Perfusion

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Following inhalation anesthesia, laser speckle contrast imaging was performed prior to flap creation, and at 0 hours, 2, 5, and 10 days after surgery, with the Full-Field Laser Perfusion Imager (Moor Instruments, Axminster, UK) in low-resolution/high-speed setting at a display rate of 25 Hz, time constant of 0.3 s, and camera exposure time of 20 ms. Processing of the contrast images produced a scaled color-coded Live Flux image (red: high perfusion; blue: low perfusion) which correlated with the blood flow velocity in the tissue.15 (link)
Data were analyzed using Moor FLPI V3.0 PC Software (Moor Instruments). The flap was divided into cranial, central, and caudal regions of interest (ROIs) of equal-imaged surface area. Data from each ROI were exported to R Statistical Analysis software (R Foundation, Vienna, Austria). To characterize the baseline microvascular anatomy of lean and obese mice, perfusion data from the surgically created flap were selected and exported to Matlab R2013b software (Mathworks Inc., Natick, MA, USA). A three-dimensional surface plot was constructed from the matrix data with floor set to average preoperative perfusion to illustrate the flap changes relative to the unoperated state.13

Clark R.M., Coffman B., McGuire P.G, & Howdieshell T.R. (2016). Myocutaneous revascularization following graded ischemia in lean and obese mice. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 9, 325-336.

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Investigating CGRP Antagonist Effects on Blood Flow

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Blood flow was assessed in the ear, leg, or paw using the Full-field Laser Perfusion Imager (Moor Instruments) on anesthetized mice.^{12 (link)} To investigate the local effects of αAnalogue, mice were pretreated with the CGRP antagonist BIBN4096 (0.3 mg/kg, IV) or control (neutralized saline) followed by αAnalogue injection (100 pmol daily, ipsilateral ear) or vehicle (contralateral ear). In separate experiments, blood flow in the periphery was measured following systemic treatment of the αAnalogue.

Aubdool A.A., Thakore P., Argunhan F., Smillie S.J., Schnelle M., Srivastava S., Alawi K.M., Wilde E., Mitchell J., Farrell-Dillon K., Richards D.A., Maltese G., Siow R.C., Nandi M., Clark J.E., Shah A.M., Sams A, & Brain S.D. (2017). A Novel α-Calcitonin Gene-Related Peptide Analogue Protects Against End-Organ Damage in Experimental Hypertension, Cardiac Hypertrophy, and Heart Failure. Circulation, 136(4), 367-383.

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Tissue Perfusion and Sulfide Assay

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Tissue perfusion rates were performed daily via Full‐Field Laser Perfusion Imager (Moor Instruments). The mice were then euthanized; soleus and gastrocnemius muscle tissues were harvested from CBS^+/− and WT mice treated with or without H₂S. Free tissue sulfide levels were assayed in two different muscles (tibialis anterior and gastrocnemius) at the end of the treatment using methods reported before(Rivers et al., 2012 (link)). Plasma levels of Hcy were also determined by the HPLC‐UV method (Amarnath et al., 2003 (link)). We also assessed the changes in the angiogenic program by protein arrays from all the groups to identify the vasculogenic changes. The red blood cell (RBCs) density was also measured to know the effects of H₂S treatment on the blood flow in the limb of CBS^+/− mice. As RBCs contain a high concentration of hemoglobin (Hb), which binds reversibly with O₂ thus the amount of O₂ released in the peripheral tissue, including the muscle depends on the quantity of the circulating RBCs (Cabrales et al., 2008 (link)).

Singh M., Pushpakumar S., Zheng Y., Homme R.P., Smolenkova I., Mokshagundam S.P, & Tyagi S.C. (2022). Hydrogen sulfide mitigates skeletal muscle mitophagy‐led tissue remodeling via epigenetic regulation of the gene writer and eraser function. Physiological Reports, 10(16), e15422.

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