Anti flag affinity resin a2220

Rabbit polyclonal antibody (pAb) against Myc (R1208-1), GAPDH (glyceraldehyde-3-phosphate dehydrogenase; EM1101), and β-actin (EM21002) were purchased from Huaan Biological Technology (Hangzhou, China). Mouse anti-HA (H3663) and anti-Flag (F1804) MAbs were both obtained from Sigma-Aldrich (USA). Anti-Flag affinity resin (A2220) for immunoprecipitation was also purchased from Sigma-Aldrich. Rabbit pAb against ubiquitin (ab7780) were purchased from Abcam (USA). Rabbit anti-K63 (D7A11) and anti-K48 (D9D5) MAbs were obtained from Cell Signaling Technology (USA). Rabbit pAb to VP1 of IBDV, chicken pAb to VP3 of IBDV, and mouse MAb to VP1, VP2, VP3, VP4, and VP5 of IBDV were produced by our laboratory (17 (link), 51 (link), 52 (link)). MG132 (S2619) was purchased from Selleckchem (USA). Cycloheximide (CHX) was obtained from Medchemexpress (HY-12320). N-ethylmaleimide (NEM), a deubiquitination inhibitor, was obtained from Sigma-Aldrich (E3876). Cell NP-40 lysis buffer (50 mM Tris [pH 7.4], 150 mM NaCl, 1% NP-40) was purchased from Beyotime (P0013F; Shanghai, China). Horseradish peroxidase (HRP)- or fluorescein isothiocyanate (FITC)-labeled goat anti-mouse and anti-rabbit IgG were purchased from KPL (Milford, MA). FITC-labeled goat anti-chicken antibodies were purchased from Abcam (ab6873). Alexa Fluor 546-conjugated anti-mouse or anti-rabbit IgG were obtained from Invitrogen (USA).

Rabbit polyclonal antibody (pAb) against Myc (R1208-1), GFP (SR48-02), FLAG (0912-1), and mouse monoclonal antibody (mAb) against β-actin (M1210-2), GST (M0807-1) were purchased from Huaan Biological Technology (Hangzhou, China). Mouse anti-Myc (05-419) and anti-FLAG (F1804) mAbs were both obtained from Sigma-Aldrich (United States). Anti-FLAG affinity resin (A2220) for immunoprecipitation was also purchased from Sigma-Aldrich. Mouse monoclonal antibody (mAb) against GFP (B-2, sc-9996) was purchased from Santa Cruz Biotechnology. Glutathione agarose beads for GST pull-down were purchased from Thermo Scientific (United States). Mouse mAb to Cap of PCV2 was produced by our laboratory (Zhou et al., 2005 (link)). Cell NP-40 lysis buffer [50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40] was purchased from Beyotime (P0013F; Shanghai, China). Horseradish peroxidase (HRP)-labeled goat anti-mouse and anti-rabbit IgG were purchased from KPL (Milford, MA, United States).

Mouse monoclonal antibodies (mAbs) against GST (M0807-1), histone H3 (R1105-1), and β-actin (M1210-2), as well as rabbit polyclonal antibodies (pAbs) against Myc (R1208-1), Flag (0912-1), β-tubulin (0807-2), and GFP (SR48-02) were purchased from Huaan Biological Technology (Hangzhou, China). Mouse anti-Flag (F1804) and anti-Myc (05–419) mAbs were obtained from Sigma-Aldrich (St. Louis, MO, United States). Rabbit mAb against DDX21 (ab182156) was obtained from Abcam (Cambridge, MA). Anti-Flag affinity resin (A2220) for immunoprecipitation was purchased from Sigma-Aldrich. NP-40 cell lysis buffer (50 mM Tris [pH 7.4], 150 mM NaCl, 1% NP-40) was obtained from Beyotime (P0013F; Shanghai, China). Horseradish peroxidase (HRP)-labeled goat anti-mouse and anti-rabbit IgG or fluorescein isothiocyanate (FITC)-labeled goat anti-mouse IgG were purchased from KPL (Milford, MA, United States). Alexa Fluor 546-conjugated donkey anti-rabbit IgG was obtained from Invitrogen (United States).