Seongsanamide A (>98%, Figure 1 ) was isolated from the fermentation broth extracts of Bacillus sp. KCTC 12796BP by as previously reported [7 (link)]. Acetaminophen, amodiaquine, bupropion, chlorzoxazone, dextromethorphan, trimipramine, uridine diphosphoglucuronic acid (UDPGA), nicotinamide adenine dinucleotide phosphate (NADP+), glucose-6-phosphate (G6P), and G6P dehydrogenase (G6PDH) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Coumarin, midazolam, omeprazole, and tolbutamide were purchased from Toronto Research Chemicals (Toronto, ON, Canada). Pooled HLMs (XTreme 200) were supplied by XenoTech (Lenexa, KS, USA). We purchased rP450 isoforms (rCYP1A2, rCYP2A6, rCYP2B6, rCYP2C8, rCYP2C9, rCYP2C19, rCYP2D6, rCYP2E1, rCYP2J2, rCYP3A4, and rCYP3A5) from Corning life sciences (Woburn, MA, USA). All solvents used in the analyses were LC-MS grade (Fisher Scientific Co., Pittsburgh, PA, USA).
Trimipramine
Manufactured by Merck Group
Sourced in United States
Trimipramine is a tricyclic antidepressant medication used in the treatment of depression. It functions as a norepinephrine-dopamine reuptake inhibitor (NDRI).
Automatically generated - may contain errors
Lab products found in correlation
Bupropion, by Merck Group (3 mentions)
Chlorzoxazone, by Merck Group (2 mentions)
Dextromethorphan, by Merck Group (2 mentions)
Tolbutamide, by LGC (2 mentions)
Midazolam, by LGC (2 mentions)
Amodiaquine, by Merck Group (2 mentions)
Coumarin, by LGC (2 mentions)
Clomipramine, by Merck Group (2 mentions)
Omeprazole, by LGC (1 mentions)
Lc ms grade, by Thermo Fisher Scientific (1 mentions)
Acetaminophen, by Merck Group (1 mentions)
G6p dehydrogenase g6pdh, by Merck Group (1 mentions)
Pooled hlms xtreme 200, by Xenotech (1 mentions)
Glucose 6 phosphate (g6p), by Merck Group (1 mentions)
Imipramine, by Merck Group (1 mentions)
Verapamil, by Merck Group (1 mentions)
Phenacetin, by Merck Group (1 mentions)
Omeprazole, by Merck Group (1 mentions)
7 ethoxyresorufin, by Merck Group (1 mentions)
β nicotinamide adenine dinucleotide phosphate nadp, by Merck Group (1 mentions)
4 protocols using trimipramine
1
Seongsanamide A Biosynthesis and Assay
2
Quantitative Analysis of Tricyclic Antidepressants
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Imipramine, trImipramine, clomipramine, verapamil, and nortriptiline were purchased from Sigma Chemical Co. (St. Louis, MO). Methanol, hexane, isoamyl alcohol and acetonitrile were purchased from Caledon (Ontario, Canada). All reagents and solutions were either HPLC or analytical grades. Imipramine 25 mg tablets (Batch No:555) manufactured by Lorestan pharmaceutical company (Iran), and Tofranil® 25 mg tablets (Batch No:8006) was from Novartis (Switzerland).
3
Enzyme Inhibition Assay Protocols
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The following chemicals were obtained from Sigma-Aldrich (St. Louis, MO, USA): amodiaquine, bupropion, chlorzoxazone, dextromethorphan, 7-ethoxyresorufin, glucose-6-phosphate (G6P), G6P dehydrogenase (G6PDH), β-nicotinamide adenine dinucleotide phosphate (NADP+), omeprazole, phenacetin, and trimipramine. Coumarin, midazolam, and tolbutamide were obtained from Toronto Research Chemicals (Toronto, ON, Canada). We purchased resorufin from Tokyo Chemical Industry (Tokyo, Japan). Pooled human liver microsomes (pHLMs, XTreme 200) were obtained from XenoTech (Kansas City, KS, USA), and human recombinant CYP1A1 (rCYP1A1) and CYP1A2 (rCYP1A2) isoforms were obtained from SPMED (Busan, Republic of Korea). Chrysophanol (95.0% purity), emodin (94.3% purity), obtusifolin (95.4% purity), physcion (95.0% purity), and rubrofusarin (90.0% purity) (Figure 1 ) were kindly provided by the Korea Institute of Science and Technology (Gangneung, Republic of Korea).
4
Analytical Standards for Neuropharmacological Compounds
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Sample preparation was performed with analytical grade solvents obtained from Merck KGaA (Darmstadt, Germany). LC-MS grade acetonitrile for the mobile phase was obtained from Fisher Scientific GmbH (Schwerte, Germany), and water and formic acid (99% purity) were purchased from Acros Organics (Geel, Belgium). The following deuterated standards (99% purity) were purchased from LGC Standards GmbH (Wesel, Germany): 6 Furthermore, the following standards (99% purity) were purchased from LGC Standards GmbH (Wesel, Germany) or Sigma Aldrich (St. Louis, MO): 7-aminoflunitrazepam, alprazolam, amisulpride, amitriptyline, aripiprazole, benperidol, bromazepam, bupropion, chlorpromazine, chlorprothixene, citalopram, clomipramine, clonazepam, clozapine, diazepam, doxepin, flunitrazepam, fluoxetine, fluphenazine, flupentixol, f l u v o x a m i n e , h a l o p e r i d o l , h y d r o x y r i s p e r i d o n e , levomepromazine, maprotiline, melperone, methylphenidate, mianserin, midazolam, mirtazapine, norcitalopram, norclomipramine, norclozapine, nordiazepam, nordoxepin, nortriptyline, norvenlafaxine, olanzapine, opipramol, oxazepam, paroxetine, pipamperone, promethazine, quetiapine, sulpiride, temazepam, trazodone, trimipramine, venlafaxine, and zuclopenthixol. A standard mixture containing 10 ng/μL of each analyte in methanol was prepared and diluted as required.
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