Placental pericytes (Pl-PCs; PromoCell) and bone marrow derived mesenchymal stromal cells (BM-MSCs; PromoCell) were thawed and seeded into untreated/uncoated flasks. Pl-PCs were seeded at a density of 3–4 × 104 cells per cm2 into 1 T-150 flask. Pl-PCs were cultured and expanded in Pericyte Growth Medium (PromoCell). BM-MSCs were seeded at a density of 4 × 104 cells per cm2 into 1 T-150 flask. BM-MSCs were cultured and expanded in Mesenchymal Stem Cell Growth Medium (PromoCell). Both Pl-PC and BM-MSC cultures were fed once every 2–3 days and cells passaged at 80–90% confluency.
Pl pcs
Manufactured by PromoCell
The PL-PCs is a laboratory equipment that performs cell culture plating. It is designed to accurately and efficiently seed cells into multi-well plates for various cell-based experiments and assays.
Automatically generated - may contain errors
1 protocol using pl pcs
1
Expansion of Placental Pericytes and Bone Marrow Mesenchymal Stromal Cells
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