PTEN subcellular distribution in COS-7 cells was determined by immunofluorescence using mouse monoclonal anti-PTEN 425.A79 (link) and fluorescein-conjugated anti-mouse antibody (Thermo Fisher Scientific). For quantitation of PTEN subcellular distribution, at least 50 positive cells were scored for each experiment. Cells were rated as nuclear staining (N), cytoplasmic staining (C), or staining within both the nucleus and the cytoplasm (N/C). Nuclei were identified by DAPI staining.
Fluorescein conjugated anti mouse antibody
Manufactured by Thermo Fisher Scientific
Sourced in United States
Fluorescein-conjugated anti-mouse antibody is a laboratory reagent used for the detection and identification of mouse-derived proteins or antigens in biological samples. The antibody is conjugated with the fluorescent dye fluorescein, which allows for the visualization and quantification of target molecules using fluorescence-based techniques, such as flow cytometry or fluorescence microscopy.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)
Hrp conjugated anti rabbit antibody, by Thermo Fisher Scientific (1 mentions)
Rapamycin rapa, by Merck Group (1 mentions)
Rabbit anti p62 antibody, by Merck Group (1 mentions)
Mouse β actin antibody, by Merck Group (1 mentions)
Mouse anti p53, by Santa Cruz Biotechnology (1 mentions)
Mouse anti parp1 antibody, by BD (1 mentions)
Horseradish peroxidase hrp conjugated anti mouse antibody, by Thermo Fisher Scientific (1 mentions)
Olaparib, by Selleck Chemicals (1 mentions)
Bafilomycin a1, by Merck Group (1 mentions)
3 methyladenine, by Merck Group (1 mentions)
Hydrogen peroxide, by Merck Group (1 mentions)
2 protocols using fluorescein conjugated anti mouse antibody
1
PTEN Subcellular Localization in COS-7 Cells
2
Autophagy Regulation via mTOR and p53
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Numerous reagents were purchased commercially. Bafilomycin A1, hydrogen peroxide, 3-methyl adenine, β-nicotinamide mononucleotide (β-NMN), chloroquine (CQ), wortmannin, sodium iodate (SI), 50% glutaraldehyde, rapamycin (RAPA), a rabbit anti-p62 antibody, and a mouse β-actin antibody were purchased from Sigma-Aldrich (USA); a horseradish peroxidase (HRP)-conjugated anti-mouse antibody, a HRP-conjugated anti-rabbit antibody, a fluorescein-conjugated anti-mouse antibody and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Thermo Fisher Scientific (USA); a mouse anti-PARP1 antibody was purchased from BD Biosciences (USA); rabbit anti-LC3, anti-lysosomal-associated membrane protein (LAMP1), anti-phospho mammalian target of rapamycin (mTOR) (serine 2248), anti-mTOR, and anti-acetyl p53 (lysine 382) antibodies were purchased from Cell Signaling Technology (USA); rabbit anti-AIF, mouse anti-p53, and mouse anti-RPE65 antibodies were purchased from Santa Cruz Biotechnology (USA); a mouse anti-PAR antibody was purchased from Enzo Life Sciences (USA) and Abcam (England); and olaparib was purchased from Selleck Chemicals (USA).
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