Yeast extract

All organic solvents (methanol, ethanol, acetone, dimethyl formamide (DMF) and, N,N-diisopropylethylamine (DIPEA)) were HPLC grade (Fisher Scientific) and used without further purification. E. coli cells (BL21 and NEB 5-alpha) were purchased from New England Biolabs. Lysozyme and Sephadex LH-20 were purchased from Sigma-Aldrich. BLUEStain protein ladder and ampicillin were purchased from Gold Biotechnology. Yeast extract, L-proline, tryptone, glycerol, and other bacterial culture media components were purchased from Research Products International. All materials for SDS-PAGE were obtained from Bio-Rad. PE/Cyanine7 anti-human EGFR antibody was purchased from BioLegend. Cy5.5 NHS ester was obtained from Lumi Probe. DMEM/F12, DMEM, fetal bovine serum (FBS), PenStrep, and other cell culture reagents were obtained from Corning Life Sciences.

All yeast strains and plasmids (Supplementary file 1) were manipulated using standard techniques (Amberg et al., 2005 ). Yeast cells were cultivated in liquid or on solid agar plates of rich or synthetic media, as appropriate to maintain plasmid selection. Rich growth medium was YPD (1% yeast extract (#Y20020, Research Products International Corp., Mount Prospect, IL), 2% peptone (#P20241, RPI Corp.), 2% dextrose (#G32045, RPI Corp.)). Synthetic growth medium was based on YC (0.1 g/L Arg, Leu, Lys, Thr, Trp, and uracil; 0.05 g/L Asp, His, Ile, Met, Phe, Pro, Ser, Tyr, and Val; 0.01 g/L adenine; 1.7 g/L Yeast Nitrogen Base (YNB) without amino acids or ammonium sulfate; 5 g/L ammonium sulfate; 2% dextrose) with individual components (from Sigma Aldrich, St. Louis, MO, or RPI Corp.) eliminated as appropriate for plasmid selection. For solid media, agar (#A20030, RPI Corp.) was added to 2%. G418 sulfate (Geneticin, #G1000, US Biological) was added to YPD at 200 µg/mL for selection of kanMX. Mycophenolic acid (MPA) was added to YPD to a final concentration of 100 µg/mL of the active drug. Sporulation was induced in 1% potassium acetate, 0.05% glucose, 20 mg/L leucine, 40 mg/L uracil. Bacterial strain DH5alpha was used to propagate plasmids. Yeast transformation was performed using the Frozen-EZ Yeast Transformation Kit II (Zymo Research).