Ab243074

The spinal cords were fixed with 4% paraformaldehyde (PFA), embedded in paraffin, and cut into slices of 4 μm thickness. After deparaffinization and rehydration, hematoxylin-eosin (HE) and Luxol Fast Blue (LFB) staining were performed as previously described [19 (link)]. For fluorescence immunohistochemistry, the sections were incubated overnight with mouse monoclonal primary antibody against IBA1 (Servicebio, GB12105, 1:150) at 4 °C, followed by staining with a donkey anti-mouse IgG secondary antibody (Abcam, ab150108, 1:200). For fluorescence immunocytochemistry, the cells were harvested after gently repeated blowing, centrifuged at 1000 rpm, fixed with 4% PFA, permeabilized with 0.1% Triton ×100, and incubated with primary mouse monoclonal anti-MGO antibody (Abcam, ab243074, 1:200) at 4 °C overnight, followed by staining with donkey anti-mouse IgG secondary antibody (Abcam, ab150108, 1:200). All images were captured using Olympus BX51 or LEICA DMi8 microscope.