A Superdex 200 prep grade 10/200 GL size exclusion column (GE Healthcare, Piscaway, USA) was connected to an Agilent 1100 Series (Agilent Technologies, Santa Clara, USA) and equilibrated with SEC low salt running buffer (50 mM HEPES, pH 7.0) at 1.0 mL/min. 100 μL of sample was injected und absorbance was monitored at 280 nm. The concentration of protein was determined by comparison to a calibration curve prepared from a standard solution of the same protein. The amount of nanoparticle was only determined relatively to the feed.
Superdex 200 prep grade 10 200 gl size exclusion column
Manufactured by GE Healthcare
Sourced in United States
The Superdex 200 prep grade 10/200 GL size exclusion column is a laboratory equipment product designed for biomolecule separation. It is used for the purification and analysis of proteins, peptides, and other macromolecules based on their size and molecular weight.
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Lab products found in correlation
2 protocols using superdex 200 prep grade 10 200 gl size exclusion column
1
Size Exclusion Chromatography for Protein Analysis
2
Size Exclusion Chromatography Purification
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A Superdex 200 prep grade 10/200 GL size exclusion column (GE Healthcare) or a self-packed Sephacryl300 10/200 column (GE Healthcare) was connected to an ÄKTA-explorer system (GE Healthcare) and equilibrated with SEC low salt running buffer (50 mM HEPES, pH 7.0) at 1.0 mL/min. 200 μL or 1000 μL of sample was injected, the absorbance was monitored at 280 nm and the peaks were collected.
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