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Recombinant amphiregulin

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Recombinant Amphiregulin is a protein produced using recombinant DNA technology. It is a member of the epidermal growth factor (EGF) family and plays a role in cell proliferation and differentiation.

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Lab products found in correlation

Anti pt308 akt, by Cell Signaling Technology (1 mentions) Recombinant human egf, by Thermo Fisher Scientific (1 mentions) Anti py1068 egfr, by Cell Signaling Technology (1 mentions) Anti egfr, by Enzo Life Sciences (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti py705 stat3, by Cell Signaling Technology (1 mentions) Anti ps473 akt, by Cell Signaling Technology (1 mentions) Anti erk1 2, by Merck Group (1 mentions) Anti stat3, by Cell Signaling Technology (1 mentions) Anti histone h3, by Cell Signaling Technology (1 mentions) Anti cyclin d1, by Santa Cruz Biotechnology (1 mentions) Anti p erk1 2, by Cell Signaling Technology (1 mentions) Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (1 mentions) Protein g sepharose beads, by Zymo Research (1 mentions) Matrigel, by BD (1 mentions)

4 protocols using recombinant amphiregulin

1

Macrophage Polarization by Amphiregulin

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Macrophages cells were isolated from the lungs of Poly I:C treated Foxp3YFPCre mice. These cells were incubated with recombinant Amphiregulin (R&D) (10 µg/ml). 72 hours later, the expression of different M1 and M2 markers were measured by flow cytometry.
Harb H., Benamar M., Lai P.S., Contini P., Griffith J.W., Crestani E., Schmitz-Abe K., Chen Q., Fong J., Marri L., Filaci G., Del Zotto G., Pishesha N., Kolifrath S., Broggi A., Ghosh S., Gelmez M.Y., Oktelik F.B., Cetin E.A., Kiykim A., Kose M., Wang Z., Cui Y., Yu X.G., Li J.Z., Berra L., Stephen-Victor E., Charbonnier L.M., Zanoni I., Ploegh H., Deniz G., De Palma R, & Chatila T.A. (2021). Notch4 signaling limits regulatory T-cell-mediated tissue repair and promotes severe lung inflammation in viral infections. Immunity, 54(6), 1186-1199.e7.
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2

Amphiregulin Modulates Macrophage Polarization

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Macrophages cells were isolated from the lungs of Poly I:C treated Foxp3YFPCre mice. These cells were incubated with recombinant Amphiregulin (R&D) (10 μg/ml). 72 hours later, the expression of different M1 and M2 markers were measured by flow cytometry.
Harb H., Benamar M., Lai P.S., Contini P., Griffith J.W., Crestani E., Schmitz-Abe K., Chen Q., Fong J., Marri L., Filaci G., Del Zotto G., Pishesha N., Kolifrath S., Broggi A., Ghosh S., Gelmez M.Y., Oktelik F.B., Cetin E.A., Kiykim A., Kose M., Wang Z., Cui Y., Yu X.G., Li J.Z., Berra L., Stephen-Victor E., Charbonnier L.M., Zanoni I., Ploegh H., Deniz G., De Palma R, & Chatila T.A. (2021). Notch4 signaling limits regulatory T-cell-mediated tissue repair and promotes severe lung inflammation in viral infections. Immunity, 54(6), 1186-1199.e7.
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3

Ovariectomy and Amphiregulin Treatment

Cited 1 time
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At 8–12 weeks of age, mice were anesthetized with an intramuscular injection of ketamine (80 mg/kg) and xylazine (8 mg/kg) cocktail and ovaries were removed bilaterally as previously described [25 (link)]. All animals were given two weeks to recover prior to infection. Recombinant amphiregulin (10μg; R&D) was administered intraperitoneally every other day using saline as the vehicle.
Hall O.J., Limjunyawong N., Vermillion M.S., Robinson D.P., Wohlgemuth N., Pekosz A., Mitzner W, & Klein S.L. (2016). Progesterone-Based Therapy Protects Against Influenza by Promoting Lung Repair and Recovery in Females. PLoS Pathogens, 12(9), e1005840.
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4

Fas receptor signaling in EGFR-mediated cell growth

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The sources of antibodies and reagents are as followed: anti-pY291 Fas (prepared as previously described13 and validated for immunoblotting13 and flow cytometry Fig. S8); anti-Src, anti-Yes (R&D Systems); anti-human Fas (C20, for immunoblotting) and anti-Cyclin D1 (Santa Cruz); anti-Fas (Apo1–3, for co-immunoprecipitation) (Enzo); anti-EGFR, anti-pY1068 EGFR, anti-pY705 STAT3, anti-STAT3, anti-pS473 Akt, anti-pT308 Akt, anti-Akt, anti-pErk1/2, and anti-Histone H3 (Cell Signaling Technology); anti-Erk1/2 (Sigma); anti-GAPDH (Calbiochem); horseradish peroxidase conjugated anti-rabbit and anti-mouse (Jackson ImmunoResearch); anti-mouse IgG-Alexa Flour 488 (Life Technology); Recombinant human EGF (Gibco), recombinant amphiregulin (R&D Systems); protein G sepharose beads (Zymed Laboratories); WST-1 (Interchim); Matrigel (BD Bioscience). Lipofectamine RNAiMAX reagent (Invitrogen) was used for siRNA transfection according to the manufacturer’s protocol for reverse transfection.
Ta N.L., Chakrabandhu K., Huault S, & Hueber A.O. (2018). The tyrosine phosphorylated pro-survival form of Fas intensifies the EGF-induced signal in colorectal cancer cells through the nuclear EGFR/STAT3-mediated pathway. Scientific Reports, 8, 12424.
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