Anti h3k27ac 39133

Manufactured by Active Motif

Sourced in United States

Anti-H3K27ac (39133) is a laboratory reagent used in epigenetic research. It is a monoclonal antibody that specifically binds to the acetylated form of histone H3 at lysine 27 (H3K27ac). This modification is associated with active enhancers and promoters. The antibody can be used in various applications, such as chromatin immunoprecipitation (ChIP), western blotting, and immunofluorescence.

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Lab products found in correlation

Ab23980, by Abcam (2 mentions) Lncap cell, by American Type Culture Collection (1 mentions) Anti gata2 sc 9008, by Santa Cruz Biotechnology (1 mentions) Anti foxa1 ab23738, by Abcam (1 mentions) Anti h3k4me3 07 473, by Merck Group (1 mentions) Anti cdk2 sc 163, by Santa Cruz Biotechnology (1 mentions) Rabbit igg i5006, by Merck Group (1 mentions) Anti ha ha 11 clone 16b12, by Fortrea (1 mentions) Na934v, by GE Healthcare (1 mentions) Na931v, by GE Healthcare (1 mentions) Ab7028, by Abcam (1 mentions) Ab17721, by Abcam (1 mentions) Sc 28356x, by Santa Cruz Biotechnology (1 mentions) Ab7029, by Abcam (1 mentions) Ab32631, by Abcam (1 mentions) Ab133264, by Abcam (1 mentions) Sc 2020, by Santa Cruz Biotechnology (1 mentions) Sc 365644, by Santa Cruz Biotechnology (1 mentions) Sc 1616, by Santa Cruz Biotechnology (1 mentions) Sc 2025, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

H3K27ac (82 citations) Anti-H3K27ac (34 citations)

4 protocols using anti h3k27ac 39133

LNCaP Cell Culture and Antibody Usage

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LNCaP cells were obtained from American Type Culture Collection and passaged in our laboratory for less than 6 months after resuscitation. LNCaP cells are maintained in RMPI 1640 culture medium supplemented with 10% fetal bovine serum in a 37°C humidified chamber supplemented with 5% CO₂. Antibodies used in this study include anti- H3K27ac (39133) from Active Motif, anti-FOXA1 (ab23738) from Abcam, anti-AR (sc-815X), anti-GATA2(sc-9008) and anti-HOXB13(sc-66923) from Santa Cruz

Jin H.J., Jung S., DebRoy A.R, & Davuluri R.V. (2016). Identification and validation of regulatory SNPs that modulate transcription factor chromatin binding and gene expression in prostate cancer. Oncotarget, 7(34), 54616-54626.

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Antibody Validation for Protein Analysis

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The following antibodies were used: anti-SKI (sc-9140, Santa Cruz); anti-RUNX1 (ab23980, Abcam); anti-CDK2 (sc-163, Santa Cruz); anti-Flag (F3163, Sigma); anti-HA (HA.11 clone 16B12; Covance); anti-H3K4me3 (07-473, Millipore); anti-H3K27ac (39133, Active Motif); rabbit IgG (I5006, Sigma).

Feld C., Sahu P., Frech M., Finkernagel F., Nist A., Stiewe T., Bauer U.M, & Neubauer A. (2018). Combined cistrome and transcriptome analysis of SKI in AML cells identifies SKI as a co-repressor for RUNX1. Nucleic Acids Research, 46(7), 3412-3428.

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Immunoprecipitation and Western Blotting Workflow

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The antibodies for immunoprecipitation were anti-LSD1 (ab17721, Abcam, Cambridge, UK), anti-GFI1B (sc-28356X, Santa Cruz Biotech, Dallas, TX, USA), anti-RUNX1 (ab23980, Abcam), anti-H3K27ac (39133, Active Motif, Carlsbad, CA, USA), anti-CoREST (ab32631, Abcam), normal rabbit IgG (sc-2027, Santa Cruz), and normal mouse IgG (sc-2025, Santa Cruz). The primary antibodies for western blotting were anti-LSD1 (C69G12, Millipore, Billerica, MA, USA), anti-CoREST (ab32631, Abcam), anti-HDAC1 (ab7028, Abcam), anti-HDAC2 (ab7029, Abcam), anti-GFI1B (sc-28356X or sc-22795, Santa Cruz), anti-RUNX1 (ab23980, Abcam, or sc-365644, Santa Cruz), anti-ERG (ab133264, Abcam), and anti-ACTIN (sc-1616, Santa Cruz). The secondary antibodies were anti-rabbit IgG (NA934V, GE Healthcare, Little Chalfont, UK), anti-mouse IgG (NA931V, GE Healthcare), and anti-goat IgG (sc-2020, Santa Cruz) conjugated with horseradish peroxidase.

Yamamoto R., Kawahara M., Ito S., Satoh J., Tatsumi G., Hishizawa M., Suzuki T, & Andoh A. (2018). Selective dissociation between LSD1 and GFI1B by a LSD1 inhibitor NCD38 induces the activation of ERG super-enhancer in erythroleukemia cells. Oncotarget, 9(30), 21007-21021.

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Chromatin Immunoprecipitation and Analysis

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ChIP assays were performed as previously described (Xiao et al., 2015 (link)). All primers used in ChIP-PCR are displayed in Table S1. Anti-H3Ac (39139), anti-H4Ac (39243), anti-H3K27Ac (39133; all from Active Motif), anti-H3K9Ac (ab4441; Abcam), and anti-Flag (F1804; Sigma-Aldrich) were used for the immunoprecipitation of chromatin with an EZ ChIP kit according to the manufacturer’s instructions (Millipore). The precipitated DNA was then analyzed by real-time PCR. Data are presented as relative binding based on normalization to input DNA (Staudt et al., 2010 (link)).

Zhang X., Xiao X., Lan P., Li J., Dou Y., Chen W., Ishii N., Chen S., Xia B., Chen K., Taparowsky E, & Li X.C. (2018). OX40 Costimulation Inhibits Foxp3 Expression and Treg Induction via BATF3-Dependent and Independent Mechanisms. Cell reports, 24(3), 607-618.

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