Enoxaparin

Manufactured by Sandoz

Enoxaparin is a low-molecular-weight heparin (LMWH) that is used as a laboratory reagent. It is a highly purified fraction of heparin and has a lower molecular weight compared to unfractionated heparin. Enoxaparin is used in research and testing applications as an anticoagulant.

Automatically generated - may contain errors

Lab products found in correlation

Prothrombin, by Enzyme Research (3 mentions) Human fxa, by Enzyme Research (3 mentions) Spectrozyme th, by Sekisui (2 mentions) Spectrozyme fxa, by Sekisui (2 mentions) 2 aminoacridone, by Merck Group (1 mentions) Deae sepharose fast flow resin, by GE Healthcare (1 mentions) Sodium cyanoborohydride, by Merck Group (1 mentions) Hbs ep buffer, by Cytiva (1 mentions) Streptavidin sa sensor chips, by Cytiva (1 mentions) Tributylamine, by Merck Group (1 mentions)

4 protocols using enoxaparin

Purification and Activity of Heparin Lyase II

Check if the same lab product or an alternative is used in the 5 most similar protocols

Heparin lyase II, originating from Flavobacterium heparinum, was expressed and purified in Escherichia coli (no EC assigned) in our laboratory as described previously. Pharmaceutical heparins were selected having various levels of anticoagulant activity (units of USP activity) from Celsus (Lot: PH-85314), Scientific Protein Laboratories (SPL2, Lot: 1037-3465 and BL4, Lot: 002-139), Hepar (BL2, Lot: 38988C), and Upjohn (BL6, Lot: BU3888B). LMWHs were from Sanofi-Aventis (Lovenox^®, enoxaparin, Lot: 4LM92) Teva (enoxaparin, Lot: AB15027), and Sandoz (enoxaparin, Lot: 927791). The ion-pairing reagent tributylamine was purchased from Sigma-Aldrich (St. Louis, MO). All other chemicals were of HPLC grade.

Chen Y., Lin L., Agyekum I., Zhang X., St Ange K., Yu Y., Zhang F., Liu J., Amster I.J, & Linhardt R.J. (2016). Structural Analysis of Heparin-Derived 3-O-Sulfated Tetrasaccharides: Antithrombin Binding Site Variants. Journal of pharmaceutical sciences, 106(4), 973-981.

+ Open protocol

+ Expand

Chemoenzymatic Synthesis of 13C-labeled Heparan Sulfate

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

An enoxaparin reference standard was purchased from SANDOZ. 2-Aminoacridone (AMAC) and sodium cyanoborohydride were purchased from Sigma-Aldrich (St. Louis, MO, USA). Three ¹³C-labeled 8-mer calibrants were synthesized using the chemoenzymatic synthesis method.^{10 (link)} Recombinant heparan lyase I, II, and III were expressed in E. coli and purified by a Ni-agarose column. DEAE-Sepharose Fast Flow resin was purchased from GE Healthcare (Chicago, IL, USA). All reagents and chemicals were high-performance LC (HPLC) grade or LC-mass spectrum (MS) grade.

Wang Z., Arnold K., Dhurandahare V.M., Xu Y., Pagadala V., Labra E., Jeske W., Fareed J., Gearing M, & Liu J. (2022). Analysis of 3-O-Sulfated Heparan Sulfate Using Isotopically Labeled Oligosaccharide Calibrants. Analytical chemistry, 94(6), 2950-2957.

+ Open protocol

+ Expand

Heparin and Enoxaparin Binding Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Heparin was purchased from Celsus Laboratories (Cincinnati, OH). Enoxaparin from Sandoz was generously provided by Dr. Jawed Fareed in Loyala University Medical Center (Maywood, IL). AT and human PF4 were purchased from Hyphen BioMed (Neuville-sur-Oise, France). Streptavidin (SA) sensor chips and HBS-EP buffer were purchased from Cytiva.

Zhang W., Jin W., Pomin V.H., Zhang F, & Linhardt R.J. (2022). Interactions of marine sulfated glycans with antithrombin and platelet factor 4. Frontiers in Molecular Biosciences, 9, 954752.

+ Open protocol

+ Expand

Anticoagulant Reagents and Enzyme Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

UFH (MW ~12000–16000Da) was from Sigma Aldrich (St. Louis, MO), fondaparinux (MW = 1728Da) from Apotex (Weston, FL), enoxaparin (MW ~5000Da) from Sandoz (Princeton, NJ) and dalteparin (MW ~5000Da) from Eisai (Woodcliff Lake, NJ). ODSH (Fryer, et al 1997 (link)) was a gift from J.A. Voynow (Virginia Commonwealth University). FV810^QQ, an altered form of FVa in which the B-domain residues 811–1490 are absent and the thrombin cleavage sites at Arg709 and Arg1545 have been mutated to Gln (Bos and Camire 2012 (link)), was a gift from R.M. Camire (University of Pennsylvania). Human FXa and prothrombin were from Enzyme Research Laboratories (South Bend, IN). Thrombin, the thrombin inhibitor dansylarginine N-(3-ethyl-1,5-pentanediyl)amine (DAPA) and corn trypsin inhibitor (CTI) were from Haematologic Technologies (Essex Junction, VT). Recombinant TFPIα was as described (Lockett and Mast 2002 (link)), and an inhibitory monoclonal antibody directed against the second Kunitz domain of TFPIα (anti-K2) was a gift from Novo Nordisk (Bagsvaerd, Denmark). TF (Dade Innovin) was from Siemens (Washington, DC). Thrombin and FXa chromogenic substrates (Spectrozyme TH and Spectrozyme FXa, respectively) were from Sekisui Diagnostics (Lexington, MA).

Wood J.P., Baumann Kreuziger L.M., Desai U.R, & Mast A.E. (2016). Blocking Inhibition of Prothrombinase by Tissue Factor Pathway Inhibitor alpha: A Procoagulant Property of Heparins. British journal of haematology, 175(1), 123-132.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!