Pchk2 ser 19

Manufactured by Cell Signaling Technology

Sourced in United States

PCHK2 Ser 19 is a lab equipment product offered by Cell Signaling Technology. It is a phospho-specific antibody that recognizes endogenous levels of CHK2 protein when phosphorylated at serine 19.

Automatically generated - may contain errors

Lab products found in correlation

Cytokeratin 10, by Santa Cruz Biotechnology (1 mentions) Bradford assay reagent, by Bio-Rad (1 mentions) Sirt3, by Merck Group (1 mentions) Sirt1, by Cell Signaling Technology (1 mentions) Sirt2, by Abcam (1 mentions) Involucrin, by Santa Cruz Biotechnology (1 mentions) P nbs1 ser343, by Cell Signaling Technology (1 mentions) P atm ser1981, by Cell Signaling Technology (1 mentions) Ac p53, by Cell Signaling Technology (1 mentions) Immobilon fl, by Merck Group (1 mentions) Ecl plus, by GE Healthcare (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Actin a5441, by Merck Group (1 mentions) Supersignal west dura, by Thermo Fisher Scientific (1 mentions) Erk1 2 06 182, by Merck Group (1 mentions) Cyclin d3, by Cell Signaling Technology (1 mentions) P chk2 thr68, by Cell Signaling Technology (1 mentions) Proliferating cell nuclear antigen (pcna), by Cell Signaling Technology (1 mentions) Ecl chemocam imager, by Intas (1 mentions)

2 protocols using pchk2 ser 19

Quantitative Protein Analysis Protocol

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Whole cell lysates were collected using RIPA buffer with added protease inhibitor cocktail (Roche). Insoluble fractions were collected using Urea-containing buffer. Protein was quantified using Bradford assay reagent (Biorad) and bovine serum albumin standards (Pierce). Equal amounts of protein were electrophoresed in SDS-PAGE gels and transferred to PVDF membrane Immobilon-FL (Millipore). Blots were developed using ECL-plus (GE) for 5 minutes, chemiluminescence visualized on a Licor Odyssey FL imager and quantitation done using Licor Image Studio software. All quantitated protein signals were normalized to GAPDH signal from the same gel. Antibodies used were as follows: SIRT1 (Cell Signaling), SIRT2 (Abcam), SIRT3 (Sigma), Involucrin (Santa Cruz), Cytokeratin 10(Santa Cruz), GAPDH (Santa Cruz), p-NBS1 Ser 343 (Cell Signaling), NBS1 (Santa Cruz), ac-p53 (Cell Signaling), p53 (Calbiochem), pATM Ser 1981 (Cell Signaling), ATM(Cell Signaling), pCHK2 Ser 19 (Cell Signaling), CHK2(Cell Signaling).

Langsfeld E.S., Bodily J.M, & Laimins L.A. (2015). The Deacetylase Sirtuin 1 Regulates Human Papillomavirus Replication by Modulating Histone Acetylation and Recruitment of DNA Damage Factors NBS1 and Rad51 to Viral Genomes. PLoS Pathogens, 11(9), e1005181.

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Western blot analysis of cell cycle proteins

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Neuroendocrinology 2018;106:58-73 DOI: 10.1159/000463386 61 cyclin D3 (#2936), CDK4 (#12790), CDK6 (#13331), Chk1 (#2360), pChk2 (Ser19) (#2666), pChk2 (Thr68) (#6334), Chk2 (#6334), Parp (#9542), PCNA (#2586) (all from Cell Signaling Technology, Danvers, MA, USA), p16 INK4A (ab151303) (Abcam, Cambridge, UK), Rb (#614602) (Biolegend, San Diego, CA, USA), actin (A5441) (Sigma, St. Louis, CA, USA), and ERK1/2 (06-182) (Merck-Millipore, Darmstadt, Germany). After washing in TBS, the membranes were incubated with a peroxidase-conjugated secondary antibody (1: 25,000) for 2 h. The blots were washed and immersed in the chemiluminescent substrate Super Signal West Dura (Thermo-Scientific), and images were taken with an ECL Chemocam Imager (INTAS, Göttingen, Germany).

Aristizabal Prada E.T., Nölting S., Spoettl G., Maurer J, & Auernhammer C.J. (2018). The Novel Cyclin-Dependent Kinase 4/6 Inhibitor Ribociclib (LEE011) Alone and in Dual-Targeting Approaches Demonstrates Antitumoral Efficacy in Neuroendocrine Tumors in vitro. Neuroendocrinology, 106(1).

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