Sunitinib was obtained from Selleck Chemicals (USA), and cRGDfK peptide was synthesized by Dr. Park (CHA Meditech Co., Ltd, Korea). Recombinant human TGF-β1 was purchased from R&D Systems, Inc. (USA). Fetal bovine serum (FBS), Dulbecco’s Modified Eagle’s Medium (DMEM), and antibiotics (100 U/mL penicillin and 100 μg/mL streptomycin) were purchased from Corning, Inc. (USA). Antibodies against p-ERK1/2, ERK1/2, p-Smad2/3, and Smad2/3 were purchased from Cell Signaling Technology, Inc. (USA). Antibodies against E-cadherin, N-cadherin, vimentin, TNIK, β-catenin, lamin B1, horseradish peroxidase (HRP)-conjugated secondary antibodies, and HRP-conjugated actin were purchased from Santa Cruz Biotechnology, Inc. (USA), and α-smooth muscle actin (α-SMA) was purchased from Abcam (UK).
Antibodies against e cadherin
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Antibodies against E-cadherin are a type of laboratory reagent used for the detection and analysis of the E-cadherin protein. E-cadherin is a cell adhesion molecule that plays a crucial role in maintaining cell-cell junctions and epithelial tissue integrity. These antibodies can be used in various research applications, such as immunohistochemistry, Western blotting, and flow cytometry, to study the expression and localization of E-cadherin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
N cadherin, by Santa Cruz Biotechnology (3 mentions)
1 2 dimyristoyl rac glycero 3 methoxypolyethylene glycol 2000 mpeg dmg, by NOF corporation (3 mentions)
Vimentin, by Santa Cruz Biotechnology (2 mentions)
Smad2 3, by Cell Signaling Technology (2 mentions)
P smad2 3, by Cell Signaling Technology (2 mentions)
P akt, by Cell Signaling Technology (2 mentions)
Sunitinib, by Selleck Chemicals (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions)
Hrp conjugated actin, by Santa Cruz Biotechnology (1 mentions)
Recombinant human tgf β1, by R&D Systems (1 mentions)
β catenin, by Santa Cruz Biotechnology (1 mentions)
Lamin b1, by Santa Cruz Biotechnology (1 mentions)
α smooth muscle actin α sma, by Abcam (1 mentions)
Mucin 5ac, by Merck Group (1 mentions)
Icg 001, by Selleck Chemicals (1 mentions)
Axin2, by Cell Signaling Technology (1 mentions)
Matrigel invasion chamber, by BD (1 mentions)
Trypsin neutralization solution, by Lonza (1 mentions)
Vascular endothelial growth factor (vegf), by R&D Systems (1 mentions)
Trypsin edta, by Lonza (1 mentions)
6 protocols using antibodies against e cadherin
1
Sunitinib and cRGDfK Peptide Modulate TGF-β1 Signaling
2
Antibodies for Cell Migration Analysis
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Anti-integrin α2 (ΙΙΕ10), α3 (X8), α6 (ELE) and β1 (P5D2), along with anti-tetraspanin CD151 (5C11), CD9 (C9BB) and CD82 (M104), were raised in-house as described in prior studies [19 (link)]. The CD151-specific monoclonal antibody used for IHC analyses was obtained from Leica Microsystems, Inc. (Buffalo Grove, IL). Antibodies against fibronectin and Mucin 5Ac were purchased from Sigma-Aldrich (St. Louis, MO). Antibodies recognizing total and phospho-Akt or MAPK or GSK3-β, along with those against Snail, Slug, LRP5, LPR6 and Axin-2, were obtained from Cell Signaling Technology (Danvers, MA). Antibodies against E-cadherin, Twist, Zeb1 and Zeb2 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Matrigel invasion chambers and anti-β4 integrin antibody were purchased from BD Biosciences (Franklin Lake, NJ). ICG-001, an inhibitor of canonical Wnt signaling, was obtained from Selleckchem (Houston, TX).
3
Quantifying Angiogenesis Through Immunohistochemistry
4
Exploring Epithelial-Mesenchymal Transition
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Recombinant human TGF-β1 (Cat: 100-21) was purchased from the PeproTech Corporation (Cranbury, NJ, USA). Antibodies against E-cadherin (Cat: Sc-7870), N-cadherin (Cat: Sc-8424), Vimentin (sc-58899) and GAPDH(Sc-47724) were obtained from the Santa Cruz Biotechnology (Dallas, TX, USA). The crystal violet staining solution (Cat: C0121) and the ChIP assay kit (Cat: P2078) were purchased from the Beyotime Biotechnology (Shanghai, China). The lipidoid C12-200 was purchased from the Xinjiahecheng Medical Chemistry Corporation (Wuhan, Hubei, China). 1,2-Dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (mPEG-DMG) was ordered from the NOF Corporation (Tokyo, Japan). The CCK-8 assay kit (Cat: 40203ES60) was originated from Yeasen Biotechnology (Wuhan, China), and the Lipofectamine 3000 (Cat: L3000015) was obtained from the Invitrogen Corporation (Shanghai, China). All other reagents were obtained from Sigma (St. Louis, MO, USA).
5
Cell Line Maintenance and Antibody Validation
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The SCLC cell lines NCI-H446 and NCI-H1688 were purchased from the Cellular Biology Institute of the Shanghai Academy of Sciences (Shanghai, China). Both cell lines were maintained in RPMI-1640 medium supplemented with 100 U/mL penicillin, 100 μg/mL phytomycin, and 10% fetal calf serum (FCS), and were cultured in a humidified atmosphere in an incubator at 37°C and with 5% CO2. RPMI-1640, PBS RPMI-1640, and FCS were purchased from Gibco-BRL (Life Technologies, Paisley, Scotland). Antibodies against total AKT, p-AKT, smad2/3, and p-smad2/3 were all obtained from Cell Signaling Technology, Inc. (CST, CA, USA). Antibodies against E-cadherin, cyclinD1, vimentin, caspase-3, PARP, TGF-β, MMP9, and Flot1 were supplied by Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).
6
Nobiletin Regulates Glioblastoma Cell Viability
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Reagents. Nobiletin, LY294002, TGF-β1 was from Merck Millipore (Darmstadt, Germany). AZD1080, SKL2001 and diamidino-phenyl-indole (DAPI) were purchased from Funakoshi (Tokyo, Japan). Slug plasmid (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was transfected into U343 cells according to the manufacturer's protocol. Antibodies against p38, p-p38, ERK, p-ERK, p-AKT, AKT, β-catenin, GSK-3β, p-GSK-3β, p-Smad3 were from Cell Signaling Technology (Danvers, MA, USA). Antibodies against E-cadherin, N-cadherin, occluding, fibronectin, Slug, Twist1, and Snail were obtained from Santa Cruz Biotechnology.
Cell culture and viability assay. U87, U251 and U43 cells from the American Type Culture Collection (ATCC, Manassas, VA, USA) were maintained at 37˚C, 5% CO 2 , in DMEM supplemented with 10% fetal bovine serum, penicillin (500 U/ml) and streptomycin (100 µg/ml). Cell proliferation was determined using the CCK-8 (Beyotime Institute of Biotechnology, Shanghai, China) method. U87 and U251 cells (3x10 3 cells/well) were plated in 96-well plates. At 24 h incubation with Nobiletin, CCK-8 reagents were added and incubated for another two hours. The absorbance at 450 nm represents the number of viable cells with a Multiskan Spectrum microplate reader.
Cell culture and viability assay. U87, U251 and U43 cells from the American Type Culture Collection (ATCC, Manassas, VA, USA) were maintained at 37˚C, 5% CO 2 , in DMEM supplemented with 10% fetal bovine serum, penicillin (500 U/ml) and streptomycin (100 µg/ml). Cell proliferation was determined using the CCK-8 (Beyotime Institute of Biotechnology, Shanghai, China) method. U87 and U251 cells (3x10 3 cells/well) were plated in 96-well plates. At 24 h incubation with Nobiletin, CCK-8 reagents were added and incubated for another two hours. The absorbance at 450 nm represents the number of viable cells with a Multiskan Spectrum microplate reader.
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