Primary antibodies used include
mouse IgG2B anti-Acetylated tubulin (Sigma, T6793, 1:800), mouse IgG1 anti-Zrf-1 (ZIRC, Zrf1, 1:20), mouse IgG1 anti-Zrf-2 (ZIRC, Zrf2, 1:100), mouse IgG1 anti-Zrf-3 (ZIRC, Zrf3, 1:200), mouse IgG1 anti-Zrf-4 (ZIRC, Zrf4, 1:100),
chicken anti-mCherry (Millipore, AB356481, 1:1000),
rabbit anti-GFP (Invitrogen A-11122 1:600),
mouse IgG2b anti-HuC/D (Invitrogen, A21271, 1:250), and
mouse IgG1 anti-pH3 (Cell Signaling Technology, 9706S, 1:500). Secondary antibodies used include goat anti-rabbit 488 (Invitrogen, 1:200),
goat anti-mouse Igg1 647 (Invitrogen, 1:200), goat anti-mouse IgG1 488 (Invitrogen, 1:200),
goat anti-mouse IgG1 594 (Invitrogen, 1:200),
goat anti-mouse IgG2b Alexa 647 (Invitrogen, 1:200)
goat anti-mouse IgG2b 594 (Invitrogen, 1:200),
goat anti-mouse IgG2b 488 (Invitrogen, 1:200), and
goat anti-chicken 594 (Invitrogen, 1:200) .
Schnabl J., Litz M.P.H., Schneider C., PenkoffLidbeck N., Bashiruddin S., Schwartz M.S., Alligood K., Devoto S.H, & Barresi M.J.F. (2021). Characterizing the diverse cells that associate with the developing commissures of the zebrafish forebrain. Developmental neurobiology, 81(5).