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Nci h1563

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The NCI-H1563 is a human lung cancer cell line derived from a non-small cell lung carcinoma. It is a widely used cell line for research in cancer biology and drug development.

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Lab products found in correlation

Nci h1299, by American Type Culture Collection (5 mentions) Nci h460, by American Type Culture Collection (4 mentions) Nci h1975, by American Type Culture Collection (4 mentions) Nci h2347, by American Type Culture Collection (3 mentions) Nci h441, by American Type Culture Collection (3 mentions) Heracell incubator, by Thermo Fisher Scientific (2 mentions) Nci h1650, by American Type Culture Collection (2 mentions) Nci h1385, by American Type Culture Collection (2 mentions) Nci h2066, by American Type Culture Collection (2 mentions) Nci h1734, by American Type Culture Collection (2 mentions) Nci h2228, by American Type Culture Collection (2 mentions) Calu 3, by American Type Culture Collection (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Nci h661, by American Type Culture Collection (2 mentions) Nci h1573, by American Type Culture Collection (2 mentions) Rpmi 1640, by Thermo Fisher Scientific (2 mentions) Nci h1437, by American Type Culture Collection (1 mentions) Expi293f, by Thermo Fisher Scientific (1 mentions) Nk 92, by American Type Culture Collection (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions)

6 protocols using nci h1563

1

NSCLC Cell Lines Treated with PCAIs

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All NSCLC cell lines; NCI-H1299, NCI-H1563, NCI-H460 and A549 were purchased from ATCC (Manassas, VA). The cell lines H1299, H1563 and H460 were maintained in RPMI-1640 medium with 10% heat-inactivated fetal bovine serum (FBS) and antibiotics; the A549 cell line was maintained in F-12K medium with 10% heat-inactivated fetal bovine serum and antibiotics. All cell lines were treated in base media (RPMI-1640 or F-12K) containing 5% FBS which is referred to here as treatment or experimental media. The PCAIs, NSL-BA-040, NSL-BA055, and their non-farnesylated analog NSL-100 were used for treatment. The chemical structures are as previously described [26 (link)].
Ntantie E., Fletcher J., Amissah F., Salako O.O., Nkembo A.T., Poku R.A., Ikpatt F.O, & Lamango N.S. (2017). Polyisoprenylated cysteinyl amide inhibitors disrupt actin cytoskeleton organization, induce cell rounding and block migration of non-small cell lung cancer. Oncotarget, 8(19), 31726-31744.
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2

Hypoxia Exposure of NSCLC Cells

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Human NSCLC cells (NCI-H1650, NCI-H1385, NCI-H460, NCI-H522, NCI-H661, NCI-H2126, NCI-H23, NCI-H1703, NCI-H1435, NCI-H596, NCI-H2286, NCI-H1437, NCI-H1651, NCI-H2085, NCI-H2342, NCI-H2073, NCI-H1793, NCI-H2170, NCI-H1299, NCI-H2066, NCI-H2347, NCI-H1734, NCI-H1563, NCI-H441, NCI-H1975, A549, Calu-3, NCI-H2228) were purchased from ATCC (Manassas, VA). Cells were maintained in the respective medium supplemented with 10% v/v FBS, 1% v/v penicillin-streptomycin, 1% v/v L-glutamine, in a Heracell incubator (ThermoFisher, Waltham, MA) with different pO2. The experimental conditions were set as it followed: 24 h at 20% O2 (normoxia), 24 h at 1% O2 (hypoxia), 12 h at 1% O2 followed by 12 h at 20% O2 (hypoxia/normoxia), 12 h at 1% O2 followed by 12 h at 20% O2 and 12 h at 1% O2 (hypoxia/normoxia/hypoxia or intermittent hypoxia).
Salaroglio I.C., Belisario D.C., Akman M., La Vecchia S., Godel M., Anobile D.P., Ortone G., Digiovanni S., Fontana S., Costamagna C., Rubinstein M., Kopecka J, & Riganti C. (2022). Mitochondrial ROS drive resistance to chemotherapy and immune-killing in hypoxic non-small cell lung cancer. Journal of Experimental & Clinical Cancer Research : CR, 41, 243.
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3

NSCLC Cell Lines Treated with Chemotherapeutics

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NSCLC NCI-H1299 (ATCC CRL-5803), NCI-H1563 (ATCC CRL-5875), NCI-H1437 (ATCC CRL-5872), NCI-H661 (ATCC HTB-183), NCI-H1573 (ATCC CRL-5877), and NCI-H1975 (ATCC CRL-5908) cell lines were purchased from ATCC (Virginia, MA, USA). Cells were cultured in RPMI 1640 with 5 or 10% fetal bovine serum (Gibco Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C and 5% CO2. During the experiments, cells were cultured in serum-free RPMI 1640 (basal medium, BM) or neutrophil degranulation medium prepared with RPMI 1640 (NDM). Cells were treated with 100 µM etoposide or 20 µg/mL cisplatin for 48 h.
Gründing A.R., Schneider M.A., Richtmann S., Kriegsmann M., Winter H., Martinez-Delgado B., Varona S., Liu B., DeLuca D.S., Held J., Wrenger S., Muley T., Meister M., Welte T, & Janciauskiene S. (2022). Lung Adenocarcinoma Cell Sensitivity to Chemotherapies: A Spotlight on Lipid Droplets and SREBF1 Gene. Cancers, 14(18), 4454.
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4

Hypoxia Modulation of NSCLC Cell Lines

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Human NSCLC cells (NCI-H1650, NCI-H1385, NCI-H460, NCI-H522, NCI-H661, NCI-H2126, NCI-H23, NCI-H1703, NCI-H1435, NCI-H596, NCI-H2286, NCI-H1437, NCI-H1651, NCI-H2085, NCI-H2342, NCI-H2073, NCI-H1793, NCI-H2170, NCI-H1299, NCI-H2066, NCI-H2347, NCI-H1734, NCI-H1563, NCI-H441, NCI-H1975, A549, Calu-3, NCI-H2228) were purchased from ATCC (Manassas, VA). Cells were maintained in the respective medium supplemented with 10% v/v FBS, 1% v/v penicillin-streptomycin, 1% v/v L-glutamine, in a Heracell incubator (ThermoFisher, Waltham, MA) with different pO 2 . The experimental conditions were set as it followed: 24h at 20% O 2 (normoxia), 24h at 1% O 2 (hypoxia), 12 h at 1% O 2 followed by 12h at 20% O 2 (hypoxia/normoxia), 12h at 1% O 2 followed by 12h at 20% O 2 and 12h at 1% O 2 (hypoxia/normoxia/hypoxia or intermittent hypoxia).
Salaroglio I.C., Belisario D.C., Akman M., Vecchia S.L., Godel M., Anobile D.P., Ortone G., Digiovanni S., Fontana S., Costamagna C., Kopecka J., & Riganti C. (2022). C/EBP-β LAP regulates chemo-immuno-resistance in hypoxic non-small cell lung cancer.
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5

Cell Line Maintenance and Optimization

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The K562, Raji, NCI-H441, NCI-H1563, NCI-H1299, NCI-H2347, A549, p815 and NK-92 cell lines were obtained from the American Type Culture Collection (ATCC), while the 293FT and Expi293F™ cells were purchased from Thermo Fisher Scientific (#R70007). All cells were maintained according to the provider’s recommendations and were cultured for approximately up to 20 passages. NK-92 cells were cultured in Minimum Essential Medium alpha supplemented with 15% FBS, 15% horse serum, and additional supplements, including myo-inositol (0.2 mM), 2-mercaptoethanol (0.1 mM), folic acid (0.02 mM), and interleukin (IL)-2.
Lee D.H., Ahn H., Sim H.I., Choi E., Choi S., Jo Y., Yun B., Song H.K., Oh S.J., Denda-Nagai K., Park C.S., Irimura T., Park Y, & Jin H.S. (2023). A CRISPR activation screen identifies MUC-21 as critical for resistance to NK and T cell-mediated cytotoxicity. Journal of Experimental & Clinical Cancer Research : CR, 42, 272.
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6

Lung Cell Lines and Fibroblasts Culture

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The lung cell lines (WI-38, A549, NCI-H1573, NCI-H661, NCI-H460, NCI-H1975, NCI-H1563, and NCI-H1299) and human lung fibroblasts (WI-38) cells were purchased from American Type Culture Collection (Manassas, VA). WI38 cells were cultured in Minimum Essential Medium (Invitrogen, Carlsbad, CA), A549 cells were cultured in F12 Kaighn’s Medium (Invitrogen, Carlsbad, CA) and NCI-H1573, NCI-H661, NCI-H460, NCI-H1975, NCI-H1563, and NCI-H1299 cells were cultured in RPMI 1640 (Invitrogen, Carlsbad, CA). All media were supplemented with 10% heat-inactivated fetal bovine serum (Invitrogen, Carlsbad, CA), 100 U/ml penicillin and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA). The cultures were incubated at 37°C in 5% CO2/95% humidified air. In all cases, treatment was done in basal medium supplemented with 5% heat-inactivated fetal bovine serum.
Nkembo A.T., Amissah F., Ntantie E., Poku R.A., Salako O.O., Ikpatt O.F, & Lamango N.S. (2019). Polyisoprenylated cysteinyl amide inhibitors deplete K-Ras and induce caspase-dependent apoptosis in lung cancer cells. Current cancer drug targets, 19(10), 838-851.
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