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3 protocols using kaempferol 3 o galactoside

1

Comprehensive Analysis of Phenolic Compounds

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The standards caffeic acid, caftaric acid, coutaric acid, fertaric acid, kaempferol-3-O-rutinoside, quercetin-3-rutinoside, kaempferol 3-O-galactoside, quercetin-3-glucuronide, quercetin-3-galactoside, quercetin-3-glucoside, quercetin-3-rhamnoside, kaempferol-3-O-glucoside, isorhamnetin 3-glucoside, dihydroquercetin-3,5-rhamnoside, dihydrokaempferol-3-glucoside, procyanidin trimer, procyanidin B2, procyanidin tetramer, (−)-epicatechin, (−)-epicatechin 3-gallate, petunidyn-3-O-glucoside, malvidyn-3-O-glucoside, delphinidyn-3-O-(6′-acetyl)-glucoside, petunidyn-3-O-(6″-p-coumaroyl)-glucoside and malvidyn-3-O-(6″-caffeoyl)-glucoside were purchased from Extrasynthese (Lyon Nord, France). 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid, ABTS), gallic acid, FeCl3, Trolox and all solvents were purchased from Sigma-Aldrich (Steinheim, Germany). Pectinex® Ultra SP-L was obtained from Novozymes (Bagsvaerd, Denmark).
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2

Comprehensive Phytochemical Analysis Protocol

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Acetonitrile, formic acid, methanol, all-trans-β-carotene, all-trans-lutein, all-trans-zeaxanthin, violaxanthin, chlorophyll a, chlorophyll b, chlorophyllide b, pheophytin a, pheophytin b, betulinic, oleanolic and ursolic acid, ABTS 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), methanol acetic acid, and phloroglucinol were purchased from Sigma-Aldrich (Steinheim, Germany). (-)-Epicatechin, (+)-catechin, procyanidin B2,, caffeic acid, p-coumaric acid, 3-O-caffeoylquinic, 5-O-caffeoylquinic, ferulic acid, galloyl glucose, caftaric acid, luteolin 7-O-galactoside, apigenin 7-O-glucoside, kaempferol-3-O-galactoside, quercetin-3-Orutinoside, quercetin-3-O-galactoside, quercetin-3-O-glucoside, quercetin-3-O-arabinoside, quercetin-3-O-xyloside, cis-piceid, trans-piceid, cis-resveratrol were purchased from Extrasynthese (Lyon, France). Acetonitrile for ultra-phase liquid chromatography (UPLC; Gradient grade) and ascorbic acid were purchased from Merck (Darmstadt, Germany).
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3

Antioxidant Compounds Extraction

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Acetonitrile, formic acid, betulinic, oleanolic and ursolic acid, ABTS (2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), methanol, acetic acid, and phloroglucinol were purchased from Sigma-Aldrich (Steinheim, Germany). (-)-Epicatechin, (+)-catechin, chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, dicaffeic acid, procyanidin A2, procyanidin B2, p-coumaric acid, caffeic acid, kaempferol-3-O-galactoside, quercetin-3-O-rutinoside, quercetin-3-O-galactoside, quercetin-3-O-glucoside, cyanidin-3-O-arabinoside, cyanidin-3-O-galactoside cyanidin-3-O-glucoside, peonidin-3-Ogalactoside, and delfinidyn-3-O-glucoside were purchased from Extrasynthese (Lyon, France). Acetonitrile for ultraperformance liquid chromatography (UPLC; Gradient grade) and ascorbic acid were obtained from Merck (Darmstadt, Germany).
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