Chemicals for sds page

Manufactured by Bio-Rad

Sourced in United States

Chemicals for SDS–PAGE are a set of reagents used in the process of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). These chemicals enable the separation and analysis of proteins based on their molecular weight.

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Lab products found in correlation

Restriction enzymes, by Roche (1 mentions) γ 32p atp, by Cytiva (1 mentions) Trypsin solution, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Glutamine, by Merck Group (1 mentions) Bicinchoninic acid (bca), by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle medium, by Merck Group (1 mentions) Bradford protein assay, by Thermo Fisher Scientific (1 mentions) Oxyblottm protein oxidation detection kit, by Merck Group (1 mentions) Cell culture plastics, by BD (1 mentions) Penicillin, by Merck Group (1 mentions) Streptomycin, by Merck Group (1 mentions) Cell proliferation kit 2 xtt, by Roche (1 mentions)

2 protocols using chemicals for sds page

Phosphorylation Assay with bL15Vp Peptide

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[γ-³²P]ATP (specific activity 110 TBq mmol⁻¹) was obtained from Amersham Biosciences (Uppsala, Sweden).
The bL15Vp peptide biotinyl-LKGLDIDTIQQNYTpV (Tp, phosphothreonine) was synthesized by Neosystem (Strasbourg, France).
Streptavidin-agarose magnetic beads, glutathione-sepharose resin, the catalytic subunit of protein kinase A and thrombin were obtained from Sigma (St. Louis, MO, USA).
Restriction enzymes were obtained from Roche Diagnostics (Mannheim, Germany). Pfu turbo DNA polymerase was obtained from Stratagene (La Jolla, CA, USA).
Chemicals for SDS–PAGE were obtained from Bio-Rad (Hercules, CA, USA).

Pallucca R., Visconti S., Camoni L., Cesareni G., Melino S., Panni S., Torreri P, & Aducci P. (2014). Specificity of ε and Non-ε Isoforms of Arabidopsis 14-3-3 Proteins Towards the H+-ATPase and Other Targets. PLoS ONE, 9(3), e90764.

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Quantitative Cell Culture Assay Protocol

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Dulbecco's Modified Eagle Medium (4.5 mg mL -1 glucose), penicillin, streptomycin, glutamine, trypsin solution for cell culture (0.05% (w/v)) were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France) and fetal bovine serum from Invitrogen Life technologies (Cergy Pontoise, France). Cell culture plastics were purchased from BD Falcon (Le Pont de Claix, France). Cell proliferation kit II (XTT) was obtained from Roche Diagnostics (Meylan, France). Western blot reagents and membranes were purchased from GE Healthcare (Saclay, France). The bicinchoninic acid (BCA) and Bradford protein assays were purchased from Thermo scientific (Villebon-sur-Yvette, France) and from Expedeon (San Diego, USA), respectively and chemicals for SDS/PAGE from Bio-Rad (Marnes La Coquette, France).
OxyBlot TM protein oxidation detection kit was purchased from Millipore (Molsheim, France).
All other reagents were obtained from Sigma-Aldrich, unless specified.

Desvergne A., Ugarte N., Radjei S., Gareil M., Petropoulos I, & Friguet B. (2016). Circadian modulation of proteasome activity and accumulation of oxidized protein in human embryonic kidney HEK 293 cells and primary dermal fibroblasts. Free radical biology & medicine, 94.

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