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2 protocols using rabbit anti lm antibody

1

Fluorescence Microscopy Protocols for Annexin and Listeria Detection

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Primary antibodies used were rabbit anti-Annexin A2 (#610068 from BD Biosciences), rabbit anti-GFP (#A11120 from Invitrogen), rabbit anti-Caspase 7 (#12827 from Cell Signaling), rabbit anti-Listeria (#B223021 from BD Biosciences), rabbit anti-Lm antibody (from Dr. Pascale Cossart, Institut Pasteur, France), mouse anti-phosphatidylserine (#18005 from Abcam) and mouse anti-ezrin (#35-7300 from Invitrogen). Rat anti-TIM4 blocking antibody was previously described37 . Alexa Fluor 568 Phalloidin, Annexin V-Alexa Fluor-488 and -647 conjugates and all fluorescent secondary antibodies (AlexaFluor conjugates) were from Invitrogen. DAPI (#D1306 from Invitrogen) was used at 1:2500 dilution to stain the nuclei where indicated. Cytochalasin D (#2502555; 10 μM final) and Latrunculin B (#428020; 10μg/mL final) were from Calbiochem. For transfection of HeLa cells, Xtreme Gene 9 (Roche) transfection reagent was used as per manufacturer’s protocols. LifeAct-mRFP38 was a gift from Dr. Ray Truant (McMaster University, Canada). The following siRNAs were from Sigma: Annexin A1 (#00157996), Annexin A2 (#00246294), Annexin A6 (#00063383), Caspase 7 (#00128361).
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2

Fluorescence Microscopy Protocols for Annexin and Listeria Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies used were rabbit anti-Annexin A2 (#610068 from BD Biosciences), rabbit anti-GFP (#A11120 from Invitrogen), rabbit anti-Caspase 7 (#12827 from Cell Signaling), rabbit anti-Listeria (#B223021 from BD Biosciences), rabbit anti-Lm antibody (from Dr. Pascale Cossart, Institut Pasteur, France), mouse anti-phosphatidylserine (#18005 from Abcam) and mouse anti-ezrin (#35-7300 from Invitrogen). Rat anti-TIM4 blocking antibody was previously described37 . Alexa Fluor 568 Phalloidin, Annexin V-Alexa Fluor-488 and -647 conjugates and all fluorescent secondary antibodies (AlexaFluor conjugates) were from Invitrogen. DAPI (#D1306 from Invitrogen) was used at 1:2500 dilution to stain the nuclei where indicated. Cytochalasin D (#2502555; 10 μM final) and Latrunculin B (#428020; 10μg/mL final) were from Calbiochem. For transfection of HeLa cells, Xtreme Gene 9 (Roche) transfection reagent was used as per manufacturer’s protocols. LifeAct-mRFP38 was a gift from Dr. Ray Truant (McMaster University, Canada). The following siRNAs were from Sigma: Annexin A1 (#00157996), Annexin A2 (#00246294), Annexin A6 (#00063383), Caspase 7 (#00128361).
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