Low speed centrifuge

Experimental animals are 72 male C57BL/6J mice at the age of 6–8 weeks supplied by Experimental Animal Center. Drugs are as follows: sodium ferulate (SF) injection solution (Shandong Fangming Pharmaceutical Group, China), AngII (Invitrogen, USA), TBHQ (Selleck, USA), and pentobarbital sodium (Merck, USA). Instruments are as follows: PUMP 11 Elite Injection Pump (Harvard, USA), CODA Noninvasive Blood Pressure Measurement System (Kent scientific Corporation, USA), VEVO2100 Ultrasonic Image System for Small Animals (Visual Sonic, Canada), Microscope (OLYMPUS, Japan), Microplate Reader (Biotek, USA), PCR Instrument (Thermo, USA), Low-Speed Centrifuge (Beckman Coulter, USA), and Gel DoxTM XR + Gel Imaging System (Bio-Rad, USA). Reagents are as follows: RNA extraction and PCR reagents (Roche, Switzerland), TUNEL antibody (Abcam, USA), phosphatase inhibitor (Roche, Switzerland), atrial natriuretic peptide (ANP)/transforming growth factor-β (TGF-β)/Collagen III (Col III)/bcl-2/bax/caspase-3/ERK/p-ERK/p38MAPK/p-p38MAPK/JNK/p-JNK/β-actin antibodies (Cell Signaling Technology, USA), BCA quantitation and Western Blot reagents (Invitrogen, USA), and PCR primers and reagents (Invitrogen, USA). The sequences were as shown in Table 1.