R micromass r q tof micro mass spectrometer

All the chemicals were used as
received from Sigma-Aldrich (USA), Spectrochem (India), and SRL (India).
Melting points were recorded in open capillaries and are uncorrected.
Fourier transform infrared spectra (FT-IR) were recorded in the wave
number range of 4000–400 cm^–1 and are recorded
on a Thermo Mattson Satellite FT-IR spectrophotometer. Nuclear magnetic
resonance (NMR) spectra were recorded on a Bruker UltraShield spectrometer
(400 and 100 MHz) or Bruker UltraShield spectrometer (300 and 75 MHz)
in a DMSO-d₆ solvent using TMS as an internal
standard. Mass spectra were recorded on a Waters^(R)Micromass^(R)Q-TOF Micro mass spectrometer. Solvents used were of commercial
grade and purified before use by distillation.

All of the aldehydes, malononitrile,
and C–H-activated compounds were purchased from Sigma Aldrich
(USA), Spectrochem (India), and SRL (India) and were used without
further purification. All solvents were kept sealed in airtight bottles
as well to minimize the absorption of atmospheric moisture. Moreover,
they were distilled before being used. The products were characterized
by their physical constants, comparison with authentic samples, and
IR (Thermo Mattson Satellite FT-IR spectrophotometer) and NMR spectroscopies
with a Bruker UltraShield spectrometer (400 and 100 MHz) in the DMSO-d₆ solvent using tetramethylsilane (TMS) as an
internal standard. Melting points were measured using an electrothermal
melting point apparatus in capillary tubes. Mass spectra were measured
on a Waters^(R) Micromass^(R)Q-TOF micromass spectrometer.
Light microscopy (Motic, Hongkong) was used to visualize the cells.