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3 protocols using disodium ethylenediaminetetraacetate

1

Synthesis and Characterization of Ti3AlC2

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Ti3AlC2 powder was purchased from JILIN 11 Technology Co. LTD. (China). Lithium fluoride (LiF, >99.99%), chromium(III) nitrate nonahydrate (Cr(NO3)3·9H2O, 99%), hydrochloric acid (HCL), N,N-dimethylformamide (DMF, 99%), terephthalic acid (H2BDC, TPA, 98%), benzoquinone (BQ, 98%), silver nitrate (AgNO3, 99%), carbon tetrachloride (CCl4), tret-butanol (t-BuOH), disodium ethylenediaminetetraacetate (Na2-EDTA, 99%), hydrogen peroxide (H2O2, 30%), ethanol (C2H5OH), SD, AAP, sodium hydroxide (NaOH, 98%), potassium iodide (KI, 99%), ammonium molybdate tetrahydrate ((NH4)6Mo7O24·4H2O, 99.9%), and potassium hydrogen phthalate (KHC8H4O4, 99.5%) were obtained from Sigma Aldrich. All the chemicals purchased were of analytical grade and were used without further purification.
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2

Neurochemical Assays in Rodent Models

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Imipramine hydrochloride (IMI) was purchased from Sigma (St. Louis, MO, USA). The assay kits of corticosterone (CORT), corticotrophin-releasing hormone (CRH), adrenocorticotropin (ACTH), and arginine vasopressin (AVP) were purchased from Nanjing Jiancheng Institute of Biotechnology, Nanjing, China. The standard 5-hydroxytryptamine (5-HT), dopamine (DA), and norepinephrine (NE) were purchased from Sigma (St. Louis, MO, USA). Disodium ethylenediaminetetraacetate (EDTA) and the standard amino acids including glutamic acid (Glu), aspartic acid (Asp), γ-aminobutyric acid (GABA), and taurine (Tau) were obtained from Sigma (St. Louis, MO, USA). All other reagents used were of analytical grade.
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3

Chitosan-based Bioactive Compounds Synthesis

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Low molecular weight chitosan (LCS) is a commercial material provided by Qingdao Yunzhou Biotechnology Co., Ltd. (China). It's deacetylation degree >90%, average molecular weight (Mw) of 3 kDa. Chitooligosaccharide (COS) with a molecular weight of 1.1 kDa was prepared in our laboratory by the combined degradation of microwave and acetic acid. Tris (Tris), Reduced Coenzyme I (NADH), Safranine O, Nitrotetrazolium Blue (NBT), Phenazine Potassium Sulfate (PMS), 1,1-Diphenyl-2-Trinitrophenylhydrazine (DPPH) was purchased from Sigma Chemicals Co. Disodium hydrogen phosphate dodecahydrate, sodium dihydrogen phosphate dihydrate, ferrous sulfate, disodium ethylenediaminetetraacetate (EDTA), absolute ethanol, 30% hydrogen peroxide (H2O2), 1-ethyl-(3 -Dimethylaminopropyl)carbodiimide hydrochloride (EDC·HCl), N-hydroxysuccinimide (NHS), 2-morpholinoethanesulfonic acid (MES), salicylic acid (BHA), α-naphthylacetic acid (NAA), indole-3-butyric acid (IBA). All other chemicals and reagents were of analytical grade and were used without further purification.
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