Cary 660 ftir

FTIR spectra were acquired using a Cary 660 FTIR (Agilent, USA). Parameters used were: wavelength range – 750 to 10,000 nm; no. of scans – 4; acquisition mode – Attenuated Total Reflection. The aqueous samples prepared for NMR were used for FTIR.

SEM micrographs were taken on Zeiss Supra 40. PXRD patterns were obtained from Rigaku SmartLab X-ray diffractometer. XPS spectra was obtained on Versaprobe II scanning XPS. FT-IR spectra was obtained on Agilent Cary 660 FT-IR. Absorbance and fluorescence spectra were obtained on Biotek Synergy H4 Hybrid microplate reader. Thermofisher Scientific Sorvall Legend Micro17, Thermofisher Scientific Sorvall Lynx 4000, and Beckman Coulter Allegra X-14R centrifuges were used for obtaining cell and ZIF-8 pellets. CLSM images were taken on Olympus FV3000 RS microscope. The concentration of Zn in the extracted organs was quantified using Agilent 7900 ICP-MS. Flow cytometry data were acquired on a BD LSRFortessa. Cell counting was carried out on a Thermo Countess II. Live animal imaging was performed on an IVIS Lumina III. Paraffin embedding was done on Histo-Core ARCADIA. Embedded tissues were processed with a Leica RM22335 microtome. H&E images were obtained on VS120 virtual slide microscope. Ultrapure water was filtered in lab with the ELGA PURELAB flex 2 system.

The thickness of the initial PLCL films employed for the hydrolytic degradation study (see section 3.3.) was determined by using a DektakXT Advanced System (Bruker). A force of 0.3 mg was applied on the 12.5 µm stylus to limit the damage of the samples.
The thermal properties of the samples during hydrolytic degradation were determined by DSC823^e (Mettler Toledo). Samples of ~10 mg were heated from -50 °C to 185 °C at 20 °C/min. After this first scan the sample was cooled at 20 °C/min and reheated from -50 °C to 185 °C at 20 °C/min. In this second scan the glass transition temperature (T_g) was determined from the inflection point of the heat flow curve.
The molecular weight evolution of the samples during hydrolytic degradation was measured by GPC using two Shodex KF804 columns (Waters) and the RI detector 401 (Waters) at 35 °C. Tetrahydrofuran (HPLC grade, Fisher) was used as an eluent at a flow rate of 1 mL/min and polystyrene standards (Shodex Standards, SM-105, Waters) were used to obtain a primary calibration curve.
Changes in the chemical structures of these samples during degradation were analyzed by ATR-FTIR (Cary 660 FTIR, Agilent Technologies) equipped with a DLaTGS detector, a Ge coated KBr beam splitter and a Si crystal. Spectra were taken with a resolution of 4 cm⁻¹ and were averaged over 64 scans.