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Epimark n

Manufactured by New England Biolabs
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The EpiMark N is a high-performance laboratory equipment designed for epigenetic research applications. It provides consistent and reliable results in experiments involving DNA methylation analysis and chromatin immunoprecipitation (ChIP) studies. The core function of the EpiMark N is to facilitate the efficient extraction and purification of DNA and chromatin samples, enabling researchers to obtain accurate and reproducible data for their investigations.

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Lab products found in correlation

Smarter stranded total rna seq kit, by Takara Bio (2 mentions) Dynabeads mrna purification kit, by Thermo Fisher Scientific (2 mentions) Hiseq 4000 machine, by Illumina (2 mentions) Rna clean concentration 5, by Zymo Research (2 mentions)

2 protocols using epimark n

1

m6A-seq Analysis of Murine Dendritic Cells

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated from DCs. Polyadenylated RNA was further enriched from total RNA by using Dynabeads® mRNA Purification Kit (Invitrogen). RNA samples were fragmented into ~100-nucleotide-long fragments with sonication. Fragmented RNA (100 ng mRNA or 5 μg total RNA) was performed m6A-IP following EpiMark N6-methyladenosine enrichment kit (NEB E1610S) protocol. RNA was enriched through RNA Clean&Concentration-5 (Zymo Research) and used for library generation with SMARTer Stranded Total RNA-Seq Kit (Takara). Sequencing was performed at the University of Chicago Genomics Facility on an Illumina HiSeq4000 machine in single-read mode with 50 bp per read. Sequencing reads were aligned to the mouse genome mm9 by STAR (version 2.6.0c)29 (link). The m6A-enriched regions (peaks) in each m6A-IP sample were detected by MACS2 (version 2.1.1.20160309)30 (link) with q value less than 0.01 and corresponding m6A-Input sample was used as the control. Peaks that were detected by both replicates were considered as high confident peaks. The peaks annotation and binding motif were analyzed by HOMER (version 4.9)31 (link).
Han D., Liu J., Chen C., Dong L., Liu Y., Chang R., Huang X., Liu Y., Wang J., Dougherty U., Bissonnette M., Shen B., Weichselbaum R., Xu M.M, & He C. (2019). Anti-tumor immunity controlled through mRNA m6A and YTHDF1 in dendritic cells. Nature, 566(7743), 270-274.
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2

m6A-seq Analysis of Murine Dendritic Cells

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated from DCs. Polyadenylated RNA was further enriched from total RNA by using Dynabeads® mRNA Purification Kit (Invitrogen). RNA samples were fragmented into ~100-nucleotide-long fragments with sonication. Fragmented RNA (100 ng mRNA or 5 μg total RNA) was performed m6A-IP following EpiMark N6-methyladenosine enrichment kit (NEB E1610S) protocol. RNA was enriched through RNA Clean&Concentration-5 (Zymo Research) and used for library generation with SMARTer Stranded Total RNA-Seq Kit (Takara). Sequencing was performed at the University of Chicago Genomics Facility on an Illumina HiSeq4000 machine in single-read mode with 50 bp per read. Sequencing reads were aligned to the mouse genome mm9 by STAR (version 2.6.0c)29 (link). The m6A-enriched regions (peaks) in each m6A-IP sample were detected by MACS2 (version 2.1.1.20160309)30 (link) with q value less than 0.01 and corresponding m6A-Input sample was used as the control. Peaks that were detected by both replicates were considered as high confident peaks. The peaks annotation and binding motif were analyzed by HOMER (version 4.9)31 (link).
Han D., Liu J., Chen C., Dong L., Liu Y., Chang R., Huang X., Liu Y., Wang J., Dougherty U., Bissonnette M., Shen B., Weichselbaum R., Xu M.M, & He C. (2019). Anti-tumor immunity controlled through mRNA m6A and YTHDF1 in dendritic cells. Nature, 566(7743), 270-274.
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